Sample Acceptance Criteria

Blood sample

• Properly filled Requisition forms.

• Properly labeled samples.

• Details on Requisition form should be matched with sample container details

• Proper Vacutainers

• Appropriate quantity of sample.

• ABG sample & Plasma sample should not be clotted

• Spill over the sample should not be there.

• Sample will be received within 4 hours of collection.

Urine Sample:

• Properly filled Requisition forms.

• Properly labeled samples.

• Details on Requisition form should be matched with sample container details

• Appropriate quantity of sample.

• Sample containers should not be open, they should be tightly packed.

• Sample spillage should not be there.

All specimens must have the following information on the vacutainer:

• Patient’s Name

• Ward Name

• Specific Case Registration Number (MRD Number)

• Date

Information present in requisition form must match with information present on vacutainer.

For ABG analysis specimens must be received on ice packs in pre-heparinized syringe and as early as possible.

Criteria For Sample Rejection:

Blood sample:

• Improperly filled Requisition forms.

• Improperly labeled samples.

• Details on Requisition form is not matched with sample container details

• Improper Vacutainers

• Insufficient sample volume / Overfilled sample

• Clotted sample (Whole blood/Plasma)

• Spill over sample.

• Hemolyzed sample.

• Sample received after 4 hours of collection.

• Diluted Sample

Urine Sample:

• Improperly filled Requisition forms.

• Improperly labeled samples.

• Details on Requisition form is not matched with sample container details

• Insufficient sample volume.

• Improper sample container

• Spill over sample.

If prepared serum or plasma is grossly hemolyzed.

Sample is broken or has leaked in transit.

Sample acceptance Exceptions

A.Insufficient sample volume.

• Do not reject the sample.

• Perform as many examinations as possible as per priority.

• Priority of testing will be decided by the laboratory incharge or consultant.

• Inform the customer.

• Enter remarks of insufficient volume in the report.

• All pediatric samples with insufficient volume will be received by the laboratory.

B Hemolyzed sample.

• Do not analyze SGOT, SGPT, LDH, Potassium, Bilirubin, Ammonia, Hemoglobin, Hematocrit from hemolyzed samples.

• From a less hemolyzed sample Glucose, Cholesterol, Uric acid, Urea, Creatinine, Albumin, Alkaline phosphatase, Total protein, UIBC, Sodium, Calcium can be performed.

• Take the decision of which parameters can be analyzed from this sample.

• Enter remarks of Hemolyzed samples in the report.

C If blood is collected in EDTA Vacutainer Bilirubin, Cholesterol, Triglyceride, ALT, AST, Creatinine, Urea can be performed. However it is unsuitable for Coagulation study,Enzyme assay, Hormonal assay & electrolyte analysis or methods that require electrolyte or metallic cofactors.

→ Entry of received sample and requested parameters are to be entered on software.