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                        '' Documentary procedure for Sample receipt''       

Purpose: To ensure accurate and efficient receipt and processing of samples and requests in the laboratory & standardized approach for sample receiving operations in order to ensure high sample integrity and the validity of the analytical results.

Scope: This procedure applies to all laboratory personnel involved in receiving and processing samples and requests.

Responsibility: Laboratory Assistant or Laboratory Technician: Receives and processes samples. Laboratory Manager: Oversees receipt procedure and ensures compliance Laboratory is receiving samples from all the Wards of Sir T Hospital, Bhavnagar & OPD Samples from collection center of Sir T Hospital, Bhavnagar.

Responsibility: Laboratory Technician, Laboratory Assistant, Resident doctors

Procedure: Sample & Requisition form should have all the information as per this document. 7.2.2.b Requisition Procedure

Check for Sample Acceptance & Rejection criteria in all the samples.

Sample Acceptance & Rejection Criteria BLOOD/URINE SAMPLE ACCEPTANCE CRITERIA

1. Properly filled Requisition forms.Documentary procedure for Test Requisition
2. Properly labeled samples.
3. Appropriate quantity of sample.
4. Details on Requisition form should be matched with sample container details.
5. ABG sample & Plasma sample should not be clotted.
6. Sample containers should not be open, they should be tightly packed.
7. Spill over the sample should not be there.
8. Sample will be received within 4 hours of collection.

BLOOD/URINE SAMPLE REJECTION CRITERIA

1. Improperly filled Requisition forms.
2. Improperly labeled samples.
3. Details on Requisition form is not matched with sample container details
4. Clotted sample (Whole blood/Plasma)
5. Spill over sample.
6. Sample received after 4 hours of collection.
7. Diluted Sample

Sample acceptance exception

1. Insufficient sample volume.

• Do not reject the sample.
• Perform as many examinations as possible as per priority. Priority of testing will be decided by the laboratory incharge or consultant.
• Inform the customer.
• Enter remarks of insufficient volume in the report .
• All pediatric samples with insufficient volume will be received by the laboratory.

2. Hemolyzed sample.

• Do not analyze SGOT, SGPT, LDH, Potassium, Bilirubin, Ammonia, Hemoglobin, Hematocrit from hemolyzed samples.
• From a less hemolyzed sample Glucose, Cholesterol, Uric acid, Urea, Creatinine, Albumin, Alkaline phosphatase, Total protein, UIBC, Sodium, Calcium can be performed.
• Take the decision of which parameters can be analyzed from this sample.
• Enter remarks of Hemolyzed samples in the report.

3. If blood is collected in EDTA Vacutainer Bilirubin, Cholesterol, Triglyceride, ALT, AST, Creatinine, Urea can be performed. However it is unsuitable for Coagulation study,Enzyme assay, Hormonal assay & electrolyte analysis or methods that require electrolyte or metallic cofactors.

2. Maintain the unequivocal traceability of samples by request and labelling, to a uniquely identified patient. For this follow the following procedure.

Registration of the Patients details into the LIS. On registration LIS will generate a Sample ID for the sample.