Dr Piyush Tailor nchsls:c:hematology:document

nchsls:c:hematology:document:06

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

Procedure for informing patients and users about the laboratory services NameUnique ID Edition/Date of Edition Procedure for informing patients and users about the laboratory servicesnchsls:c:Hematology:document:06 03-01-2015Preparing authorityApproving authority

nchsls:c:hematology:document:07

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

Procedure for receiving examination request NameUnique ID Edition/Date of Edition Procedure for receiving examination request nchsls:c:Hematology:document:07 03-01-2015Preparing authorityApproving authorityReview periodAll teaching staffIn charge Hematology

nchsls:c:hematology:document:08

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

Procedure for primary sample collection & handling NameUnique ID Edition/Date of Edition Procedure for primary sample collection & handling nchsls:c:Hematology:document:08 03-01-2015Preparing authorityApproving authorityReview periodAll teaching staff

nchsls:c:hematology:document:09

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

Procedure for sample transportation NameUnique ID Edition/Date of Edition Procedure for sample transportation nchsls:c:Hematology:document:09(1) 03-01-2014Preparing authorityApproving authorityReview periodAll teaching staffIn-charge Hematology1 year

nchsls:c:hematology:document:10

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

Procedure for receiving of sample NameUnique ID Edition/Date of Edition Procedure for receiving of sample nchsls:c:Hematology:document:10 03-01-2015Preparing authorityApproving authorityReview periodAll teaching staff of Pathology Department In-charge Hematology

nchsls:c:hematology:document:11

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

Procedure for pre-examination handling, preparation and storage NameUnique ID Edition/Date of Edition Procedure for pre-examination handling, preparation and storagenchsls:c:Hematology:document:11 03-01-2015Preparing authorityApproving authorityReview period

nchsls:c:hematology:document:12

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

Procedure for selection, verification and validation of examination procedures NameUnique ID Edition/Date of Edition Procedure for selection, verification and validation of examination procedures nchsls:c:Hematology:document:1203-01-2015Preparing authority

nchsls:c:hematology:document:13

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

Procedure for examination NameUnique ID Edition/Date of Edition Procedure for examinationnchsls:c:Hematology:document:13(1) 03-01-2015Preparing authorityApproving authorityReview periodAll teaching staff of pathology departmentIn-charge Hematology1 year

nchsls:c:hematology:document:15

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

Procedure for storage, retention and disposal of clinical samples NameUnique ID Edition/Date of Edition Procedure for storage, retention and disposal of clinical samplesnchsls:c:Hematology:document:1503-01-2015Preparing authorityApproving authorityReview period

nchsls:c:hematology:document:16

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

Processes requiring this procedure Post-Examination Process Purpose Purpose of procedure for reporting of results is to communicate results of examination to the clinician and patient unambiguously. Procedure Printed report * Program infoDiagnostica

nchsls:c:hematology:document:17

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

Procedure for release of report Procedure 1. All reports are entered in info.diagnostica software by data entry operator/ technician which are password protected. 2. All printed reports are verified by authorized signatories and being released by the same.

nchsls:c:hematology:document:23

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

SOP for Laboratory Information System (LIS) Hematology 1. Switch on computer. 2. Open Mozilla Firefox. 3. Go to address * Name of Software:infoDiagnostica * Software User Manual: 4. Login & Click on ‘new sample’ for entering details of patient.

nchsls:c:hematology:document:25

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

Quality Indicator of Hematology Hematology Laboratory QUALITY INDICATORS FOR CONTINUAL IMPROVEMENT

nchsls:c:hematology:document:common_sample_collection_centre

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

PROCEDURE FOR REGISTRATION (REGISTRATION WINDOW) * Patient at the Common Collection Center registration window are having their case file & request form. Request forms will be filled up by respective treating doctors. Patient should submit their request form at registration window & form will be examined, if any of the required information is lacking, and then ask correction from referring doctor.

nchsls:c:hematology:document:procedure_for_primary_sample_collection_and_handling_at_common_collection_centre

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

1.PROCEDURE FOR REGISTRATION Registration window * Patient at the Common Collection Center registration window are having their case file & request form. * Request forms will be filled up by respective treating doctors. Patient should submit their request form at registration window & form will be examined, if any of the required information is lacking, and then ask correction from referring doctor.

nchsls:c:hematology:document:sop_of_aptt_by_all_three_method

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

SOP of PROCEDURES for Activated Partial Thromboplastin Time " Activated Partial Thromboplastin Time ( By Elite pro analyzer) Purpose of examination: Activated partial thromboplastin time is a general coagulation screening test of the coagulation factors XII, XI, IX, VIII, X, V, II and fibrinogen.

nchsls:c:hematology:document:sop_of_bone_marrow_aspiration

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

Bone marrow aspiration Purpose of examination: Bone marrow aspiration smears are primarily used for differential counts and assessment of the myeloid: erythroid ratio. They are the most suitable preparation for studying cellular details and the maturation process of the hematopoietic cells and for characterizing the abnormal cells. Aspiration is particularly useful in investigating patient with suspected iron deficiency anemia, anemia of chronic disease, megaloblastic anemia and acute leukemia.…

nchsls:c:hematology:document:sop_of_bone_marrow_biopsy

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

BONE MARROW BIOPSY Purpose of examination: Bone marrow biopsy allows histological assessment of cellularity, fibrosis, infections, infiltrative diseases and disease of bone and its cells. Trephine biopsy is essential for diagnosis when dry Tap or blood tap due to fibrotic or densely cellular marrow. It is particularly useful in investigating suspected aplastic or hypoplastic anemia, lymphoma, metastatic carcinoma, myeloproliferative disorder and disease of bone.

nchsls:c:hematology:document:sop_of_cbc_by_automated_hematology_analyzers

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

SOP of CBC by automated analyzer I. Complete Blood count Complete Blood count: * PURPOSE OF EXAMINATION The examination in whole blood is used mainly for diagnosis and management of complete blood count. PRINCIPLE OF EXAMINATION " Blood sample is aspirated, measured to a predetermined volume, diluted at the specified ratio,and then fed into each transducer. The transducer chamber has a minute hole called the aperture. On both side of the aperture, there are the electrodes between whic…

nchsls:c:hematology:document:sop_of_centrifuge-hemat

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

SOP of Centrifuge * Insert the power cord & switch on the power supply. * RPM & time display will blink last run RPM & time. * Time setting : Push SET time key, the timer display starts incrementing till 99 & after that it starts from 1 again.

nchsls:c:hematology:document:sop_of_computer-hemat

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

SOP of EQUIPMENTS " PARTS OF COMPUTER" * CPU * MONITOR * PRINTER * UPS * PURPOSE: Computer is used for data entry, data analysis, record keeping and to generate computerized reports of Hematology laboratory. * LOCATION: II )Hematology processing room-1

nchsls:c:hematology:document:sop_of_criteria_for_acceptance_of_results_between_two_examiners_in_case_of_inter_laboratory_comparision_and_iqc

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

CRITERIA FOR ACCEPTANCE OF RESULTS BETWEEN TWO EXAMINERS IN CASE OF INTERLABORATORY COMPARISION and IQC CRITERIA OF ACCEPTANCE OF RESULTS FOR IQC and INTERLAB COMPARISION For Interlab comparison: 1) For peripheral smear for malaria reporting results:

nchsls:c:hematology:document:sop_of_esr_by_automated_analyzer

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

SOP for AUTOMATED ESR: PURPOSE OF EXAMINATION: ESR is a useful screening test in a routine examination of any patient. It is a useful marker for underlying inflammation. PRINCIPLE OF ESR : When well mixed venous blood is placed in a vertical tubes Erythrocytes tend to fall towards the bottom.The length of fall of the top of the column of Erythrocytes in a given interval of time is termed as ESR.

nchsls:c:hematology:document:sop_of_esr_by_manual_method_modified_westerngren

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

ESR (Modified Westergrene method) A. Purpose of examination ESR is a useful nonspecific test of inflammatory response to tissue injury, as in diseases like, Rheumatic arthritis, tuberculosis, multiple myeloma, temporal arteritis, polymyalgia rheumatica. Conventional manual ESR method is simple, cheap and not dependent on power supply. Suitable for point of care (near patient) testing. Prognostically important in Hodgkin’s disease, renal cell carcinoma.

nchsls:c:hematology:document:sop_of_house_keeping-hemat

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

ROCEDURE FOR HOUSE KEEPING Objectives: * Housekeeping is important in any work area. * A clean, well-maintained work area improves safety by preventing accidents and can enhance the overall efficiency of work performed. * This SOP provides general safety procedures for laboratory housekeeping.

nchsls:c:hematology:document:sop_of_hplc

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

SOP IDENTIFICATION AND QUANTIFICATION OF VARIOUS HEMOGLOBIN BY VARIANT II HEMOGLOBIN TESTING SYSTEM PURPOSE OF EXAMINATION: The variant II β thalassaemia short program is intended for the separation and area percent determination of haemoglobin A2 and F, and as an aid in the identification of abnormal haemoglobins in whole blood using ion exchange high performance liquid chromatography.(HPLC).

nchsls:c:hematology:document:sop_of_incubator-hemat

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

SOP for INCUBATOR 1. PURPOSE: This instrument is used for incubation of sample in Reticulocyte count test 2. LOCATION: Hematology Laboratory 3. OPERATION: • The incubator is switched on and allowed to warm up to the required temperature. • The incubator is started to use after the temperature has reached 370C.

nchsls:c:hematology:document:sop_of_malaria_rapid_antigen_detection_tests

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

SOP of PROCEDURE MALARIA ANTIGEN, PLASMODIUM VIVAX & FALCIPARUM MALARIA ANTIGEN, PLASMODIUM VIVAX & FALCIPARUM Purpose of examination: Four species of the plasmodium parasites are responsible for malaria infections in human viz. P.falciparum, P.vivax, P.ovale and P.malariae. Early detection and differentiation of malaria is important due to incidence of cerebral malaria and drug resistance associated with falciparum malaria and due to the morbidity associated with other malaria forms.

nchsls:c:hematology:document:sop_of_micropipette

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

SOP for MICROPIPETTE FORWARD PIPETTING:  Press the operating button to the first stop.  Dip the tip attached to the pipette in to the solution to a depth of about 1 cm and slowly release the operating button. Wait for a while, and then withdraw it from the liquid touching it against the edge of the reservoir to remove excess liquid adhering to the outer surface of the tip.

nchsls:c:hematology:document:sop_of_microscope_hemat

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

SOP of EQUIPMENTS BINOCULAR MICROSCOPE * PURPOSE: Binocular microscope is used for viewing Differential count, PSCM, P/S for malarial parasite, Reticulocyte count etc.. * LOCATION: Hematology reporting room and Incharge room. * OPERATION: *

nchsls:c:hematology:document:sop_of_mnpt_calculation

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

Mean Normal Prothrombin Time (MNPT) Calculation of Mean Normal Prothrombin Time (MNPT) * Mean Normal Prothrombin Time- It is geometric mean of PT of reference sample group. MNPT is the denominator for calculation of international Normalized Ratio (INR)

nchsls:c:hematology:document:sop_of_night_duty_emergency_duty_policy

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

SOP of Night/Emergency Duty Policy Night Duty(Emergency duty) Policy * One 2nd/3rd year resident doctor and two technicians are posted for emergency duty daily. Emergency hours are as following: Monday to Friday: 4pm to 8am. Saturday 1pm to Monday 8am.

nchsls:c:hematology:document:sop_of_occupational_exposure_and_post_exposure_prophylaxis-hemat

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

OCCUPATIONAL EXPOSURE AND POST-EXPOSURE PROPHYLAXIS • An “exposure” that may place a Health Care Provider (HCP) at risk of blood borne infection is defined as a percutaneous injury (e.g. needle-stick or cut with a sharp instrument), contact with the mucous membranes of the eye or mouth, contact with non-intact skin (particularly when the exposed skin is chapped, abraded, or afflicted with dermatitis), or contact with intact skin when the duration of contact is prolonged (e.g. several minutes or…

nchsls:c:hematology:document:sop_of_peripheral_smear_cell_morphology_pscm

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

PSCM(Peripheral Smear Cell Morphology) Purpose of examination Every disease affecting the cellular components of the blood can be visualized in the form of either cytologic changes in cell morphology or of altered distribution of cell types. Furthermore estimations of white blood cell, and platelet concentrations on blood smears provide an excellent check on the counts derived by either manual or electronic techniques. It is also useful tool for detection of parasites.

nchsls:c:hematology:document:sop_of_peripheral_smear_for_malaria_parasite_psmp

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

PSMP(Peripheral Smear For Malarial Parasite) Purpose of examination A number of screening tests based on immunological methods have been developed for the detection of blood parasites especially malaria, but the essential method for a definitive diagnosis remains the finding of parasites in the blood film and the identification of species by morphology. The stained smears are examined light microscopically and screened for blood parasites.

nchsls:c:hematology:document:sop_of_procedure_of_check_stability_of_retained_sample

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

SOP OF PROCEDURE TO CHECK THE STABILITY OF RETAINED SAMPLE Objective: To check stability of retained sample Requirement: Testing sample Procedure - The examined samples are stored for 24 hours at at 2-8℃ for re-examination if required. - To check the stability of retained sample, every monthly one sample is rerun after 24 hours and result is compared with previous result.

nchsls:c:hematology:document:sop_of_pt_by_all_three_method

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

SOP of PT by all 3 methods " Prothrombin Time PROTHROMBIN TIME (By ELITE PRO analyzer) Purpose of examination: The prothrombin time is a coagulation screening test. It measures as a whole the activity of the coagulation factors II, V, VII, X and I

nchsls:c:hematology:document:sop_of_reticulocyte_count

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

RC ( Reticulocyte Count) A. Purpose of examination Reticulocytes are immature red cells normally present in small numbers in the blood (up to 2%). It increase when there is increase in erythropoietic activity. A reticulocyte count assesses bone marrow activity & value in monitoring the erythropoietic response of an anemic patient following treatment.

nchsls:c:hematology:document:sop_of_routine_and_emergency_investigation

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

PROTOCOL OF ROUTINE/ EMERGENCY INVESTIGATIONS 1) CBC: Outdoor samples -Run on Micros 60/Pentra XLR automated analyzer. * Indoor sample-Samples are run on Micros 60 automated analyzer/ Yumizen H500 automated analyzer * From 4 pm to 8 am, samples are run on Micros 60 automated analyzer in emergency lab.

nchsls:c:hematology:document:sop_of_sample_handling_during_covid_19

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

SAMPLE HANDLING DURING COVID 19 1. Samples are received in double packaging from transport team. 2. Sample is treated with 1% sodium hypochlorite solution/ spray. 3. Routine test is carried out as per routine SOP for handling of patient specimen.

nchsls:c:hematology:document:sop_of_solubility_test

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

SOP OF SOLUBILITY TEST Principle Sickle cell hemoglobin is insoluble in the deoxygenated state in a high molarity phosphate buffer.The crystals that form refract light and cause the solution to be turbid. Reagents Phosphate buffer Sodium dithionate powder

nchsls:c:hematology:document:sop_of_validation_of_new_lot

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

SOP of Validation of new lot of reagents Objective: To validate new lot of reagents before use Requirement: • New lot • Validated lot in use • Testing sample Procedure: • We validate every new batch of reagents by running the sample first with the old reagent and then re running it with new set of reagents and results are compared.

nchsls:c:hematology:document:sop_of_workflow_in_hematology_section

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

S.O.P. OF WORKFLOW FOR HAEMATOLOGY SECTION [A]For outdoor patients 1)First of all the patient comes at registration window with case paper & request form with desired investigations and registered at the registration desk and accordingly request forms are given filled with details of registration and desired investigations.

nchsls:c:hematology:document:standard_operating_procedures_of_hematology

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

Standard Operating procedures of Hematology * SOP Of Workflow in Hematology section * SOP Of Routine and emergency investigation * SOP Of CBC by automated hematology analyzers * SOP Of ESR by Automated analyzer * SOP Of ESR by manual method (Modified westerngren) * SOP Of Reticulocyte count * SOP Of Peripheral smear cell morphology(PSCM) * SOP Of Peripheral smear for malaria parasite(PSMP) * SOP Of Computer-Hemat * SOP Of Microscope Hemat * SOP Of Incubator-Hemat * SOP Of Micropipet…

nchsls:c:hematology:document:wdi_of_acl_elite_pro

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

WDI OF ELITE PRO (INSTRUMENT) WDI OF PT • Switch on the main power supply. • Switch on the ELITE Pro switch.(Back side of machine) • Switch on the monitor. • Monitor will display starting ACL, please wait and then system load in progress.

nchsls:c:hematology:document:wdi_of_care_and_maintenance_of_microscope-hemat

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

CARE AND MAINTENANCE OF MICROSCOPES • Handle the microscope carefully and gently to avoid sharp knocks. • Microscope should be placed at such an area which is free of dust, vibration, high temperature, moisture and direct sunlight. • Do not leave dirt, dust or finger marks on the lens surface.

nchsls:c:hematology:document:wdi_of_cbc_on_automated_analyzer_micros_60

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

WDI OF AUTOMATED HEMATOLOGY ANALYSER- MICROS-60 START: * Switch on main power supply * Switch on UPS * Switch on the cell counter by pressing the button at the rear of the instrument. * Press ID keys on front panel to enter the sample ID.

nchsls:c:hematology:document:wdi_of_cbc_on_automated_analyzer_pentra_xlr

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

WDI OF AUTOMATED HEMATOLOGY ANALYSER- PENTRA XLR START: 1. Switch on main power supply 2. Switch on UPS 3. Switchthe instrument on by pressing the button at the rear of the instrument. 4. Wait during initialization. 5. Switch on the printer. 6. After that login to application , to login into application select username and enter password.

nchsls:c:hematology:document:wdi_of_cbc_on_automated_analyzer_yumizen_h500

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

WDI OF AUTOMATED HEMATOLOGY ANALYSER- YUMIZEN START:- 1. Switch on main power supply 2. Switch on UPS 3. Switchthe instrument on by pressing the button at the rear of the instrument. 4. Wait during initialization. 5. Switch on the printer. 6. After that login to application , to login into application select username and enter password .

nchsls:c:hematology:document:wdi_of_computer_printer-hemat

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

WDI OF COMPUTER • At first, switch on the power supply. • Then switch on the CPU and Monitor in that order. • When the screen of monitor is displayed, it will require password to enter onto desktop. • Then, start any work related to the computer and save each and every work before logging off.

nchsls:c:hematology:document:wdi_of_deep_freezer

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

WDI OF DEEP FREEZE SWITCH ON PROCEDURE 1. First switch on the main power supply. 2. Switch on the deep freeze switch Store the controls for coagulation profile tests as per table given below Storage durationTemperature1 week-20°C to -22°C1 month

nchsls:c:hematology:document:wdi_of_esr_by_automated_esr_analyzer

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

WDI OF AUTOMATED ESR ANALYZER Sample Preparation: • ESR – vacuum blood collection tube contains 3.8% Sodium Citrate & vaccum to draw 1.28ml of blood. • Write down patient’s lab no. on label before sample collection Procedure: • Switch on the Analyzer unit.

nchsls:c:hematology:document:wdi_of_esr_by_modified_westergren_method

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

WDI OF MANUAL ERYTHROCYTE SEDIMENTATION RATE (ESR) (Modified Westergrene Method) * Take 0.5 ml of 3.8% sodium citrate in ESR cup. * Then add 2 ml of anticoagulated whole blood in ESR cup. * Place the Westergren pipette in the ESR cup till the blood reaches to “0” mark on ESR rack.

nchsls:c:hematology:document:wdi_of_house_keeping-hemat

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

WDI FOR HOUSE KEEPING • A cleaning routine is established for the work area daily and thorough cleaning once a month. • Area is kept as clean as the work allows. Work surfaces are kept as clean as possible, with only those items needed for the immediate project on that surface.

nchsls:c:hematology:document:wdi_of_hplc

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

WDI OF VARIANT II HEMOGLOBIN TESTING SYSTEM START UP -Switch on main power supply -Switch on UPS -Start CPU and Monitor -Open CDM 5.1 program -Switch on VCS(VARIANT II CHROMATOGRAPHIC SYSTEM) -Switch on VSS(VARIANT II SAMPLING STATION) -Click on variant II β thal Inactive mode

nchsls:c:hematology:document:wdi_of_incubater-hemat

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

WDI OF INCUBATOR SWITCH ON PROCEDURE: • First switch on the main power supply. • Switch on the incubator to start it. - The incubator has a thermostat to adjust temperature 370 C. • Place the samples for appropriate time. Note: - Do not switch off the incubator.

nchsls:c:hematology:document:wdi_of_management_of_spillage_accident_of_blood_or_infectious_material

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

MANAGEMENT OF SPILLS OR ACCIDENTS OF BLOOD / INFECTIOUS MATERIAL IN THE LABORATORY. PROCEDURE: In case of spills or accidents the following steps are essential and to be followed Wear gloves ⇓ Cover the spillage with paper towel/absorbent material* ⇓ Pour hypochlorite 1% ⇓ …

nchsls:c:hematology:document:wdi_of_manual_pt_aptt

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

WDI OF MANUAL METHOD WDI OF PT • Bring the reagent to the room temperature. • Prewarm the reagent to 370 C. • Add 0.1 ml of plasma to the test tube and place test tube and place the tube in water bath for 3-5 mins at 370 C. • Forcibly add 0.2 ml of LYOPLASTIN reagent and simultaneously start the stop watch. Shake tube to mix Content.

nchsls:c:hematology:document:wdi_of_micropipette

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

WDI FOR MICROPIPETTE PIPETTING STEPS: ◦ Press the operating button to the first stop. ◦ Dip the tip attached to the pipette in to the solution to a depth of about 1 cm and slowly release the operating button. Wait for a while, and then withdraw it from the liquid touching it against the edge of the reservoir to remove excess liquid adhering to the outer surface of the tip.

nchsls:c:hematology:document:wdi_of_occupational_exposure_and_post_exposure_prophylaxis

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

WDI OF OCCUPATIONAL EXPOSURE AND POST-EXPOSURE PROPHYLAXIS An “exposure” that may place a Health Care Provider (HCP) at risk of blood borne infection is defined as a percutaneous injury (e.g. needle-stick or cut with a sharp instrument), contact with the mucous membranes of the eye or mouth, contact with non-intact skin (particularly when the exposed skin is chapped, abraded, or afflicted with dermatitis), or contact with intact skin when the duration of contact is prolonged (e.g. several minut…

nchsls:c:hematology:document:wdi_of_preparation_of_1_sodium_hypochlorite

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

PREPARATION OF 1% SODIUM HYPOCHLORITE SOLUTION MATERIALS REQUIRED • Measuring Cylinder, 5% Sodium Hypochlorite Solution, Water METHOD OF PREPARATION • Take 200ml of 5% Sodium Hypochlorite solution in measuring cylinder • Add 800ml of water in it

nchsls:c:hematology:document:wdi_of_preparation_of_blood_smear

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

WDI FOR PREPARATION OF BLOOD SMEAR THIN SMEAR: • Take a clean & dry slide • Transfer a small drop of blood near the edge of the slide • Place a spreader slide at angle of 30 degree, pull back the spreader until it touches the drop of blood. Let the blood run along the edge of the spreader.

nchsls:c:hematology:document:wdi_of_refrigerator-hemat

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

WDI OF REFRIGERATOR SWITCH ON PROCEDURE: • First switch on the main power supply. • Switch on refrigerator switch. RUN PROCEDURE: • Adjust the temperature at 2-6°C temperature by using control panel. Note: - Do not switch off the Refrigerator

nchsls:c:hematology:document:wdi_of_reticulocyte_count

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

WDI OF RETICULOCYTE STAINING PROCEDURE Preparation of RC stain: New Methylene Blue -------- 1.0 gm Sodium Chloride --------------0.89 gm Distilled Water ---------------100 ml • Take reticulocyte diluting fluid out of the refrigerator and bring it to the room temperature.

nchsls:c:hematology:document:wdi_of_ricoh_printer

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

WDI OF RICOH PRINTER START • Switch on the power supply • Switch on start key of the printer • Check for page by opening front paper loading tray • Give command for printing documents STOP • Switch off the printer switcH • Switch off electric power switch

nchsls:c:hematology:document:wdi_of_safety_measures_in_fire

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

SAFETY MEASURES IN CASE OF FIRE • Fire extinguisher is mounted outside the washing room so that it is readily accessible to employees. • Only approved extinguisher is used. • Maintain extinguisher in a fully charged and operable condition and keep in their designated places at all times except during use.

nchsls:c:hematology:document:wdi_of_sample_receiving-hemat

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

WDI of Sample Receiving ACCEPTANCE CRITERIA: Following data must be filled up in request form – 1) Name & Registration number 2) Patient’s MRD Number (Unique ID number) 3) Age & Sex 4) Referral OPD & Unit 5) Short clinical history 6) Name of investigation

nchsls:c:hematology:document:wdi_of_stago_analyser

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

WDI OF STAGO (INSTRUMENT) WDI OF PT • Switch on the main power supply. • Switch on the STAGO switch • Switch on the monitor • Global verification is done automatically and on completion GLOBAL VERIFICATION DONE is displayed on monitor

nchsls:c:hematology:document:wdi_of_staining_of_smear

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

WDI OF PREPARATION OF STAINING GIEMSA STAIN STOCK SOLUTION Buffer pH 6.8 – KH2PO4 (9.1 G) + D.W. (1016 ml) + Na2HPO4.2H2O (11.5 g) + D.W.( 984 ml) Buffer pH 7.0 - KH2PO4 (3.5 G) + D.W. (389 ml) + NaH2PO4.2H2O (7.1 g) + D.W.( 611 ml)

nchsls:c:hematology:document:wdi_of_universal_precaution

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

WDI FOR UNIVERSAL PRECAUTION 1. Protection of staff against laboratory infection * All laboratory personnel are informed of the hazards including transmission of viral infection involved in working in a laboratory. Possible route of infection may be skin abrasion or puncture or through body orifices.

nchsls:c:hematology:document:wdi_of_waterbath

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

WDI OF WATERBATH • Turn on the main power supply. • Switch on the water bath unit mains. • Adjust the temperature of water bath with given key. • Check the light : green color suggest –ON • Red color suggest – OFF • Adjust the stirrer with STIRRER knob.

nchsls:c:hematology:document:work_desk_instructions_for_hematology

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

Work Desk Instruction of Hematology * WDI of Sample receiving-Hemat * WDI of CBC on Automated analyzer (Micros 60) * WDI of CBC on Automated analyzer (Pentra XLR) * WDI of CBC on Automated analyzer (Yumizen H500) * WDI of ESR by automated ESR analyzer * WDI of ESR by Modified Westergren method * WDI of Reticulocyte count * WDI of Preparation of Blood smear * WDI of Staining of smear * WDI of Care and maintenance of microscope-Hemat * WDI of Micropipette * WDI of Incubater-Hemat * W…