Dr Piyush Tailor nchsls:c:cytopathology:document

nchsls:c:cytopathology:document:06

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

Procedure for informing patients and users about the laboratory services Header NameUnique ID Edition/Date of Edition Procedure for informing patients and users about the laboratory servicesnchsls:c:Cytopathology:document:06 03-01-2015Preparing authority

nchsls:c:cytopathology:document:07

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

Procedure of receiving examination request NameUnique ID Edition/Date of Edition Procedure of receiving examination request nchsls:c:Cytopathology:document:0703-01-2015Preparing authorityApproving authorityReview periodAll teaching staff of Pathology Department

nchsls:c:cytopathology:document:08

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

Procedure for primary sample collection & handling NameUnique ID Edition/Date of Edition Procedure for primary sample collection & handling nchsls:c:Cytopathology:document:08 03-01-2015Preparing authorityApproving authorityReview periodAll teaching staff

nchsls:c:cytopathology:document:09

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

Procedure for sample transportation NameUnique ID Edition/Date of Edition Procedure for sample transportation nchsls:c:Cytopathology:document:0903-01-2015Preparing authorityApproving authorityReview periodAll teaching staff of Pathology Department In-charge Cytopathology

nchsls:c:cytopathology:document:10

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

Procedure for receiving of sample NameUnique ID Edition/Date of Edition Procedure for receiving of sample nchsls:c:Cytopathology:document:10 03-01-2015Preparing authorityApproving authorityReview periodAll teaching staff of Pathology Department In-charge Cytopathology

nchsls:c:cytopathology:document:11

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

Procedure for pre-examination handling, preparation and storage NameUnique ID Edition/Date of Edition Procedure for pre-examination handling, preparation and storagenchsls:c:Cytopathology:document:11 03-01-2015Preparing authorityApproving authorityReview period

nchsls:c:cytopathology:document:13

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

Procedure for examination NameUnique ID Edition/Date of Edition Procedure for examinationnchsls:c:Cytopathology:document:13 03-01-2015Preparing authorityApproving authorityReview periodAll teaching staff of pathology departmentIn-charge Cytopathology

nchsls:c:cytopathology:document:15

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

Procedure for storage, retention and disposal of clinical samples NameUnique ID Edition/Date of Edition Procedure for storage, retention and disposal of clinical samplesnchsls:c:Cytppathology:document:15 03-01-2015Preparing authorityApproving authority

nchsls:c:cytopathology:document:16

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

Processes requiring this procedure Post-Examination Process Purpose Purpose of procedure for reporting of results is to communicate results of examination to the clinician and patient unambiguously. Procedure Printed report * Program infoDiagnostica

nchsls:c:cytopathology:document:17

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

Procedure for release of report Procedure 1. All reports are entered in info.diagnostica software by residents which are password protected. 2. All printed reports are verified by authorized signatories and being released by the same. 3. The printed reports are arranged according to sample id and filed in dispatch file.

nchsls:c:cytopathology:document:23

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

SOP for Laboratory Information System (LIS) Cytopathology 1. Switch on computer. 2. Open Mozilla Firefox. 3. Go to address * Name of Software:infoDiagnostica * Software User Manual: 4. Login & Click on ‘new sample’ for entering details of patient.

nchsls:c:cytopathology:document:25

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

Quality Indicator of Cytopathology 1) External and Internal Audit: * Records of external and internal audit are maintained in the laboratory with documents of the corrective actions taken. Audit records are evaluated annually and every attempt is made to prevent those non-conformities again.

nchsls:c:cytopathology:document:sop_of_afb_stain-cyto

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

SOP FOR AFB STAIN 1. PURPOSE OF EXAMINATION: To lay down the procedure for ZN stain in Cyto-pathology section at NCHLS Surat. 2. PRINCIPLE AND METHOD OF PROCEDURE USED FOR EXAMINATION: Acid fast bacilli cell wall contains waxy lipid, mycolic acid. Phenol and carbol fuscin helps dye to pass through lipid wall and attach with negatively charged ions present in bacteria. After staining with help of reducing substance cells are decolorized except acid fast bacilli.

nchsls:c:cytopathology:document:sop_of_fluid_processing

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

SOP of Fluid processing 1.PURPOSE To prepare the slide from received body fluid, sputum and BAL fluid 2.STAFF RESPONSIBLE Senior technician. 3.PRINCIPLE Sedimentation 4.REAGENT Glacial acetic acid 5.PERFORMANCE SPECIFICATION NA 6.SAMPLE Body fluid, BAL, Urine,Bladder wash, CSF

nchsls:c:cytopathology:document:sop_of_fna

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

SOP of FNA 1.PURPOSE To prepare the Smears as per standard text literature & Manufacture’s guidelines. 2.STAFF RESPONSIBLE Resident doctor 3.PRINCIPLE NA 4.PERFORMANCE SPECIFICATION NA 5.SAMPLE NA 6.TYPE OF CONTAINER NA 7.EQUIPMENT AND REAGENT USED

nchsls:c:cytopathology:document:sop_of_guidlines_for_aspiration_process_transportation_receiving_processing_and_reporting_of_sample_in_covid-19_pandemic_era

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

SOP FOR GUIDLINES FOR ASPIRATION PROCESS, TRANSPORTATION, RECEIVING, PROCESSING AND REPORTING OF SAMPLES IN COVID-19 PANDEMIC ERA Purpose Safe guarding and protecting laboratory personnel including technician, trainee, cytopathologist by minimizing exposure to COVID-19, so that they remain safe in order to able to provide continuous service.

nchsls:c:cytopathology:document:sop_of_h_e_stain-cyto

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

SOP of Hematoxytin & Eosin stain 1.PURPOSE * To prepare the H & E stains (Harris) as per standard text literature & manufacturer’s guidelines * Lay down procedure for staining the smears prepared from fluids& FNAC in Cytopathology section at NCHLS, Surat.

nchsls:c:cytopathology:document:sop_of_maintenance_of_binocular_microscope

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

SOP FOR MAINTENANCE BINOCULAR MICROSCOPE 1. PURPOSE Binocular microscope is used for viewing slides. 2. LOCATION Cytology reporting room and Incharge room. 3. OPERATION * The slide is placed on stage gently holding back the spring arm with the specimen side up and center of the smear to be examined as accurately as possible over the hole in the center of the stage. The spring arm mechanism can get easily damaged if the surface of the stage or the under surface of the slide is wet or has o…

nchsls:c:cytopathology:document:sop_of_mgg_stain-cyto

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

SOP of MGG stain 1.PURPOSE * To prepare MGG stain as per standard text literature & manufacturer’s guidelines * Lay down procedure for staining the smears prepared from fluids& FNAC in Cytopathology section at NCHLS, Surat. 2.STAFF RESPONSIBLE

nchsls:c:cytopathology:document:sop_of_pap_stain-cyto

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

SOP of PAP stain 1.PURPOSE * To prepare PAP stain as per standard text literature & manufacturer’s guidelines * Lay down procedure for staining the smears prepared from fluids& FNAC in Cytopathology section at NCHLS, Surat. 2.STAFF RESPONSIBLE

nchsls:c:cytopathology:document:sop_of_sputum_processing

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

SOP of Sputum processing 1.PURPOSE To prepare the slide from received sputum 2.STAFF RESPONSIBLE Senior technician. 3.PRINCIPLE Spread the representative part of sputum on glass slide, with circular movement 4.REAGENT NA 5.PERFORMANCE SPECIFICATION

nchsls:c:cytopathology:document:sop_of_usg_guided_fna

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

SOP of USG guided FNA 1.PURPOSE To prepare the Smears as per standard text literature & Manufacture’s guidelines. 2.STAFF RESPONSIBLE Resident doctor 3.PRINCIPLE NA 4.PERFORMANCE SPECIFICATION NA 5.SAMPLE NA 6.TYPE OF CONTAINER NA 7.EQUIPMENT AND REAGENT USED

nchsls:c:cytopathology:document:standard_operating_procedures_of_cytopathology

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

Standard Operating procedures of Cytopathology * SOP of Fluid Processing * SOP of Sputum Processing * SOP of FNA * SOP of USG guided FNA * SOP of H&E stain-Cyto * SOP of MGG stain-Cyto * SOP of PAP stain-Cyto * SOP of AFB stain-cyto * SOP of Maintenance of Binocular Microscope * SOP of Guidlines for aspiration process, transportation,receiving,processing and reporting of sample in COVID-19 pandemic era

nchsls:c:cytopathology:document:wdi_of_1_hypo-chlorite_solution_-cyto

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

PREPARATION OF 1% SODIUM HYPOCHLORITE SOLUTION MATERIALS REQUIRED Measuring Cylinder, 5% Sodium Hypochlorite Solution, Water METHOD OF PREPARATION Take 200ml of 5% Sodium Hypochlorite solution in measuring cylinder Add 800ml of water in it Dispense it in the discarding jars

nchsls:c:cytopathology:document:wdi_of_afb-cyto

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

WDI FOR AFB STAIN Take heat fixed test slide on which staining has to be performed along with positive control. Put filter paper on the slide smear and pour the CF solution over the section. Heat section with spirit lamp till steam arises. Allow it to heat for 5 minute.

nchsls:c:cytopathology:document:wdi_of_centrifuge_-cyto

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

WDI of Centrifuge Put the power cable to socket. Turn on Switch. Open the lid of centrifuge. Load the rotor with its components of tubes/ Vacutainer. Close the lid of Centrifuge. Move speed control knob clockwise from “0’ position. To stop move speed control knob to “0’ position.

nchsls:c:cytopathology:document:wdi_of_computer_printer-cyto

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

STEPS TO START COMPUTER UPS has to be kept on always. Never switch off electric supply of UPS. Switch on CPU and Monitor. Wait till all icons appear on the screen. SHUT DOWN PROCEDURE Go to start menu. Select the icon – turn off computer. Select the option shut down in the turn off window.

nchsls:c:cytopathology:document:wdi_of_cortex_mixer

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

WDI of Vortex Mixer Connect the power cable to electric socket. On switch of the socket Power on indicate by red light. Direct ModeTouch modePress switch on directPress switch on touchMachine start directlyMachine start Regulate speed by speed regulator.

nchsls:c:cytopathology:document:wdi_of_cytotek

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

WDI Of Cytotek Write number of the specimen on a clean glass slide. Place the slide in the specimen chamber holder. Place a new filter paper on the slide. Insert two tabs at the bottom of a clean specimen chamber holder. Press the top of the specimen chamber.

nchsls:c:cytopathology:document:wdi_of_fluid_processing

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

WDI of Fluid processing Take the fluid into the test tube Centrifuge it at 2000 rpm for 10 min Remove the supernant Mix the sediment with the help of vortex mixture/manually Take one drop on slide Prepare the smear, 4 wet fix for pap & H E, 1 dry fix for MGG stain.

nchsls:c:cytopathology:document:wdi_of_fluid_spillage

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

દર્દીના ઢોળાયેલા સેમ્પલને સાફ઼ કરવાની પ્રક્રીયા(fluid Spillage) ગ્લોવ્ઝ પહેરીને રુ અથવા ગોઝ પીસને ઢોળાયેલા સેમ્પલ/ મટીરીયલ પર મુકો.

nchsls:c:cytopathology:document:wdi_of_fna

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

WDI of FNA PATIENT PREPARATION A Clear explanation of procedure to patient to get his/her consent and cooperation Procedure is usually carried out with patient lying supine on a simple examination couch. Disinfect the skin using the spirit swab.

nchsls:c:cytopathology:document:wdi_of_h_e_stain_-cyto

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

WDI of H.E. Stain After preparing the smear Immediately fix it in methanol for 10 minutes. Dry the smears. First apply Haematoxylin for 2 minutes. Wash the slide 2 times in tap water. Apply Eosin for 5-10 seconds. Wash with tap water for 2 times.

nchsls:c:cytopathology:document:wdi_of_mgg_stain_-cyto

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

WDI of MGG stain After preparing the smears First dry it in air for 5 minutes (Dry Fixation) Then fix it in methanol for 10 minutes. Dilute the MGG stain with buffer (1:10). Apply the stain over the smear for 5 minute. Remove the stain from smear.

nchsls:c:cytopathology:document:wdi_of_microscope

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

WDI Of CARE AND MAINTENANCE OF MICROSCOPES 1. Handle the microscope carefully and gently to avoid sharp knocks. 2. Microscope should be placed at such an area which is free of dust, vibration, high temperature , moisture and direct sunlight. 3. Do not leave dirt, dust or finger marks on the lens surface.

nchsls:c:cytopathology:document:wdi_of_pap_stain_-_cyto

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

WDI of Pap stain Fix the smears in methanol for 10 minutes After preparing the smears. (Wet Fixation). Wash with tap water for 30 seconds. Apply Hematoxyline for 2 minutes. Wash with tap water for 2 times. Dehydrate the smears with 90% alcohol for 2times.

nchsls:c:cytopathology:document:wdi_of_pipette

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

WDI of Pipette Set the volume. Press volume lock and hold it down. Set the volume by rotating the operating button. Release volume lock. Note: do not rotate operating button without pressing volume lock. Fit the tip on to the pipettor tip cone.

nchsls:c:cytopathology:document:wdi_of_receiving_sample-cyto

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

RECEIVING SAMPLE * Servant comes to the cytology processing room with sample * Resident doctor check the request form & sample detail if everything regarding sample & request form are found ok then sample is accepted. * Resident doctor do the gross examination of the sample & write it down on the request form.

nchsls:c:cytopathology:document:wdi_of_sputum_processing

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

WDI Of Sputum processing From sputum take the bloody, discolored & solid particle not larger than the size of pea on each of four plain slides. With a clean glass slide crush the particle of sputum on each of the four slides, using the rotary motion.

nchsls:c:cytopathology:document:wdi_of_toluidine_blue_stain_-_cyto

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

WDI for Toluidine Blue stain - Cyto 1. Take the test slide with control. 2. Bring the smear to distilled water. 3. Place the slide in jar containing 0.5% Periodic acid for 5 min. 4. Wash it well in several changes of distilled water. 5. Stain with Schiff's reagent for 10-15 min.

nchsls:c:cytopathology:document:wdi_of_transportation_of_sample-cyto

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

Transportation of sample * Technician / resident doctor are bringing the slide with copline from FNA room to cytology processing room. * Then technician process the slide. * Processed slide are then given to resident doctor * They screen the slide

nchsls:c:cytopathology:document:wdi_of_universal_precaution

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

UNIVERSAL PRECAUTION 1.Protection of staff against laboratory infection * All laboratory personnel are informed of the hazards including transmission of viral infection involved in working in a laboratory. Possible route of infection may be skin abrasion or puncture or through body orifices.

nchsls:c:cytopathology:document:wdi_of_vortex_mixer

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

Vortex Mixer * Connect the power cable to electric socket. * On switch of the socket * Power on indicate by red light. Direct ModeTouch modePress switch on direct Press switch ontouchMachine start directlyMachine start * Regulate speed by speed regulator.

nchsls:c:cytopathology:document:work_desk_instructions_for_cytopathology

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

Work Desk Instructions Of Cytopathology * WDI of Centrifuge -Cyto * WDI of Cytotek * WDI of FNA * WDI of H&E stain -Cyto * WDI of MGG stain -Cyto * WDI of PAP stain - Cyto * WDI of AFB-Cyto * WDI of Microscope * WDI of Vortex Mixer * WDI of Pipette * WDI of Computer & Printer-Cyto * WDI of Universal precaution * WDI of 1% hypo-chlorite solution -Cyto * WDI of Receiving sample-Cyto * WDI of Transportation of sample-Cyto * WDI of Fluid spillage * WDI of Fluid processing * WDI of…