Dr Piyush Tailor nchsls:c:biochemistry:document

nchsls:c:biochemistry:document:0.1n_hcl_for_autowash

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

* Take 900 ml DI water in 2 liter volumetric flask * Add 16 ml and 600 microliter concentrated HCL in Di water * Do upto 2 liter and mix well

nchsls:c:biochemistry:document:0.1n_naoh_for_autowash

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

* Take 900 mi DI water in 2 liter volumetric flask * Weight 16 gm NAOH and dissolve it in 100 ml DI water * Add this NAOH in 900 ml DI water * Do upto 2 liter and mix well

nchsls:c:biochemistry:document:06

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

Header NameUnique ID Edition/Date of Edition Procedure for informing patients and users about the laboratory servicesnchsls:c:biochemistry:document:06(1) 03-01-2014Preparing authorityApproving authorityReview periodAll teaching staff of Biochemistry Department

nchsls:c:biochemistry:document:07

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

Procedure for receiving examination request Header NameUnique ID Edition/Date of Edition Procedures for receiving examination requestnchsls:c:biochemistry:document:13(1) 24-01-2022Preparing authorityApproving authorityReview periodAll teaching staff

nchsls:c:biochemistry:document:08

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

Header NameUnique ID Edition/Date of Edition Procedure for primary sample collection & handlingnchsls:c:biochemistry:document:08(1) 29-01-2022Preparing authorityApproving authorityReview periodAll teaching staff of Biochemistry DepartmentHOD Biochemistry

nchsls:c:biochemistry:document:09

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

Header NameUnique ID Edition/Date of Edition Procedure for sample collection and transportationnchsls:c:biochemistry:document:09(1) 03-01-2014Preparing authorityApproving authorityReview periodAll teaching staff of Biochemistry DepartmentHOD Biochemistry

nchsls:c:biochemistry:document:1_agarose

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

How to prepare 1% Agarose gel * Dissolve 100 mg of pure agarose in 10 ml of hippurate buffer pH 8.6. * Boil it to dissolve it completely & make a homogeneous & clear solution. * To make sure that agarose powder dissolve completely,look under the glassware in light.Be careful for your eyes.

nchsls:c:biochemistry:document:1_agarose_gel

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

How to prepare 1% Agarose gel * take 100mg of agarose powder in a flask very carefully that powder does not touch side surface of flask(prefer using funnel) * Dissolve agarose in 10 ml of hippurate buffer pH 8.6. Pour buffer slowly from the side of flask to avoid sticking of particles to side surface of flask as particles ma interfere elecrophoresis run

nchsls:c:biochemistry:document:1_agarose_with_glycine_-_tris_buffer

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

1% Agarose Gel Preparation * Dissolve 100 mg of pure agarose in 10 ml of glycine-Tris buffer ( Half Diluted) pH 8.6. * Weight entire flask along with agarose and buffer. Note it down. * Boil it to dissolve it completely & make a homogeneous & clear solution. * Weight the boiled solution and add DI water equivalent to evaporated volume.

nchsls:c:biochemistry:document:1_agarose_with_teb_buffer

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

How to prepare 1% Agarose gel * Dissolve 150 mg of pure agarose in 15 ml of TEB buffer (pH 8.6). * Boil it to dissolve it completely & make a homogeneous & clear solution. * To make sure that agarose powder dissolve completely,look under the glassware in light.Be careful for your eyes.

nchsls:c:biochemistry:document:5_glacial_acetic_acid

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

5% Acetic Acid preparation * Take 5 ml glacial acetic acid and make up to 100ml with DI water in 100ml volumetric flask.

nchsls:c:biochemistry:document:5_lugol_s_iodine

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

VIA VILI TEST -Lugol's iodine and acetic acid is asked by O.G. Dept. -This SOP helps to prepare both the reagents. -DO not prepare and dispatch these reagents without written request from H.O.D. of O.G. and permission from H.O.D Biochemistry. -For lesser number of patients prepare lesser amount of reagents.

nchsls:c:biochemistry:document:5_phenol_for_orthopedic

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

nchsls:c:biochemistry:document:10

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

Header NameUnique ID Edition/Date of Edition Procedure for receiving samplenchsls:c:biochemistry:document:10(1) 03-01-2014Preparing authorityApproving authorityReview periodAll teaching staff of Biochemistry DepartmentHOD Biochemistry1 yearPrinted copy of this document is considered uncontrolled.

nchsls:c:biochemistry:document:10_hcl

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

How to make 10% HCL * Wear gloves and mask before starting to make 10% HCL. * Use fume hood to make 10% HCL. * Take 100ml volumetric flask and put it into a beaker to prevent the spillage of concentrated HCL over the surface. * Put these all things into the fume hood chamber.

nchsls:c:biochemistry:document:11

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

Procedure for pre-examination handling, preparation and storage Header NameUnique ID Edition/Date of Edition Procedure for pre-examination handling, preparation and storagenchsls:c:biochemistry:document:11(1) 03-01-2014Preparing authorityApproving authority

nchsls:c:biochemistry:document:12

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

Procedure for selection, verification and validation of examination procedures Header NameUnique ID Edition/Date of Edition Procedure for selection, verification and validation of examination proceduresnchsls:c:biochemistry:document:12(1) 03-01-2014

nchsls:c:biochemistry:document:13

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

Procedures for examination Header NameUnique ID Edition/Date of Edition Procedures for examinationnchsls:c:biochemistry:document:13(1) 03-01-2014Preparing authorityApproving authorityReview periodAll teaching staffHOD Biochemistry1 yearPrinted copy of this document is considered uncontrolled.

nchsls:c:biochemistry:document:14

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

Procedure for review of results Header NameUnique ID Edition/Date of Edition Procedure for review of resultsnchsls:c:biochemistry:document:14(1) 03-01-2014Preparing authorityApproving authorityReview periodAll teaching staff of biochemistry department

nchsls:c:biochemistry:document:15

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

Header NameUnique ID Edition/Date of Edition Procedure for storage, retention and disposal of clinical samplesnchsls:c:biochemistry:document:15(1) 03-01-2014Preparing authorityApproving authorityReview periodAll teaching staff of Biochemistry Department

nchsls:c:biochemistry:document:16

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

Header NameUnique ID Edition/Date of Edition Procedure for reporting of results nchsls:c:biochemistry:document:16(1) 03-01-2014Preparing authorityApproving authorityReview periodAll teaching staff of Biochemistry DepartmentHOD Biochemistry1 yearPrinted copy of this document is considered uncontrolled.

nchsls:c:biochemistry:document:17

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

Header NameUnique ID Edition/Date of Edition Procedure for release of results nchsls:c:biochemistry:document:17(1) 03-01-2014Preparing authorityApproving authorityReview periodAll teaching staff of Biochemistry DepartmentHOD Biochemistry1 yearPrinted copy of this document is considered uncontrolled.

nchsls:c:biochemistry:document:18

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

Header NameUnique ID Edition/Date of Edition Procedure for personnel managementnchsls:c:biochemistry:document:18(1) 03-01-2014Preparing authorityApproving authorityReview periodAll teaching staff of Biochemistry DepartmentHOD Biochemistry1 yearPrinted copy of this document is considered uncontrolled.

nchsls:c:biochemistry:document:19

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

Header NameUnique ID Edition/Date of Edition Procedure for management of accommodation and environmental conditionsnchsls:c:biochemistry:document:19(1) 03-01-2014Preparing authorityApproving authorityReview periodAll teaching staff of Biochemistry Department

nchsls:c:biochemistry:document:20

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

Header NameUnique ID Edition/Date of Edition Procedure for management of equipmentsnchsls:c:biochemistry:document:20(1) 03-01-2014Preparing authorityApproving authorityReview periodAll teaching staff of Biochemistry DepartmentHOD Biochemistry1 yearPrinted copy of this document is considered uncontrolled.

nchsls:c:biochemistry:document:21

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

Header NameUnique ID Edition/Date of Edition Procedure for management of reagents and consumablesnchsls:c:biochemistry:document:21(1) 03-01-2014Preparing authorityApproving authorityReview periodAll teaching staff of Biochemistry DepartmentHOD Biochemistry

nchsls:c:biochemistry:document:22

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

NameUnique ID Edition/Date of Edition Procedure for selecting and evaluating referral laboratories and consultants nchsls:c:biochemistry:document:22(1) 03-01-2014Preparing authorityApproving authorityReview periodAll teaching staff of Biochemistry Department

nchsls:c:biochemistry:document:23

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

Header Procedure for laboratory information management NameUnique ID Edition/Date of Edition Procedure for laboratory information managementnchsls:c:biochemistry:document:23(1) 03-01-2014Preparing authorityApproving authorityReview periodAll teaching staff of Biochemistry department

nchsls:c:biochemistry:document:24

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

Header NameUnique ID Edition/Date of Edition Procedure for ensuring quality of examination results nchsls:c:biochemistry:document:24(1) 03-01-2014Preparing authorityApproving authorityReview periodAll teaching staff of Biochemistry DepartmentHOD Biochemistry

nchsls:c:biochemistry:document:25

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

Header Procedure for monitoring quality indicators NameUnique ID Edition/Date of Edition Procedure for monitoring quality indicatorsnchsls:c:biochemistry:document:25(1) 02-07-2014Preparing authorityApproving authorityReview periodAll teaching staffHOD Biochemistry

nchsls:c:biochemistry:document:25_oh_vit_d

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

kit literature VITROS 3600 Centrifugation, Preparing aliquot, how_to_perform_examination_in_VITROS 3600INTERNAL QUALITY CONTROLEQA For VITROS_3600 Chemianalyzer Tests

nchsls:c:biochemistry:document:a.document

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

* On master page write Equipment Name,its Hospital Serial Number and upload its its AT(purchase order)

nchsls:c:biochemistry:document:abnormal_alert_and_reference_range

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

[Abnormal Alert and Reference Range]

nchsls:c:biochemistry:document:absured_report

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

-Open 12.207.3.240 * Enter your user name and password. * Go to 'Report--->Print'. * * Enter sample Id in from_sample_id and to_sample_id.select Authorised signatory. * Click on 'Print' * Report will open in pdf format * * Click on FIle menu

nchsls:c:biochemistry:document:action_taken_when_1_3s_or_2_2s_rule_violeted

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

general guideline * 1(3S): Random and Systemic error * 2(2S): Systemic error * Random error: error in precision * Systemic error: error in accuracy Look for following non-exhaustive list of ERROR-CENTERS Integrity of QC sera * Refrigerator temperature chart

nchsls:c:biochemistry:document:ada_reagent

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

[ADA reagent] .

nchsls:c:biochemistry:document:ada_system_pack

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

nchsls:c:biochemistry:document:adenosine_deaminase

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

Principle Adenosine ------> Inosine ADA NH3 + Nitroprusside + HOCl Procedure for Adenosine Deaminase examination references[ADA reference literature]environmental and safety controls[Adenosine MSDS] [Ammonium sulfate MSDS] Preparation of ADA reagent The examination is done on Erbachem Preparation of ADA reagent for automated measurement

nchsls:c:biochemistry:document:albumin

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

Albumin examination procedure * Learn operation of Erba XL-640 and Erbachem. * Select equipment * Use Erba XL-640 as peferred equipment. * If Erba XL-640 is malfunctioning and can not be repaired within period acceptable to clinicians, use Erbachem and follow procedure for responding to Erba XL-640 failure

nchsls:c:biochemistry:document:albumin_reagent

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

Making Reagent [Albumin Reagent]

nchsls:c:biochemistry:document:alp

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

Alkaline phosphatase examination procedure * Learn operation of Erba XL-640 and Erbachem. * Select equipment * Use Erba XL-640 as peferred equipment. * If Erba XL-640 is malfunctioning and can not be repaired within period acceptable to clinicians, use Erbachem and follow

nchsls:c:biochemistry:document:alp_current_use

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

nchsls:c:biochemistry:document:alp_older

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

nchsls:c:biochemistry:document:alp_reagent

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

How to make ALP Reagent For ALP R1 fill reagent bottle's with 6 to 8 ml R1 reagent solution and R2 bottle with 3 ml R2 reagent solution.

nchsls:c:biochemistry:document:alt

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

Procedure for ALT examination * Learn operation of Erba XL-640 and Erbachem. * Select equipment * Use Erba XL-640 as preferred equipment. * If Erba XL-640 is malfunctioning and can not be repaired within period acceptable to clinicians, use Erbachem and follow

nchsls:c:biochemistry:document:amido_black_stain

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

Amido Black Dye preparation chemicalQuantityAmido black dye2.5 gmGlacial acetic acid100 mlMethenol450 mlDi water450 ml Shake it manually without stirrer for completely mixing of all constituents.

nchsls:c:biochemistry:document:amy

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

Procedure for Amylase examination * Learn operation of Erba XL-640 and Erbachem. * Select equipment * Use Erba XL-640 as preferred equipment. * If Erba XL-640 is malfunctioning, stop the examination and follow procedure for responding to Erba XL-640 failure * Read following documents references[amylase Kit literature of Transasia page 1] [amylase Kit literature of Transasia page 2] [amylase Kit literature of cormay ] [Amylase Spinreact Kit Literature] | |Note:

nchsls:c:biochemistry:document:analyse_eqa_and_iqc_results

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

Procedure For Sorting and Analyzing monthly Internal Quality Control and External Quality Control Results * Open 10.207.3.240/ * * click on LIS. Following Window will be seen. * Log in with your ID and password. Following Window will be seen.

nchsls:c:biochemistry:document:analysis_eqa_and_iqc_results

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

nchsls:c:biochemistry:document:anti_sars_cov-2_igg

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

* Following reference is used to decide weather the plasma is Hight Titer or not * [ Guidline for use of CCP in Treatment of COVID19] VITROS 3600 Centrifugation, Preparing aliquot, how_to_perform_examination_in_VITROS 3600INTERNAL QUALITY CONTROL

nchsls:c:biochemistry:document:art_kit

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

HIV Post exposure prophylaxis facility * HIV Post exposure prophylaxis kit is present in refrigerator no.17 How to use kit First Aid- At Site of Exposure * Wash exposed part with water or Normal Saline * Do not Squeeze the Wound to bleed it

nchsls:c:biochemistry:document:ast

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

Procedure for AST examination * Learn operation of Erba XL-640 and Erbachem. * Select equipment * Use Erba XL-640 as preferred equipment. * If Erba XL-640 is malfunctioning, stop the examination and follow procedure for responding to Erba XL-640 failure * Read following documents references [AST Kit literature of Spinreact] [AST Kit literature of Meril]}} Note: When a new lot is opened, verify kit literature with the uploaded version.

nchsls:c:biochemistry:document:attachments

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

How to make attachment in any Sample * Open 10.207.3.240. * Enter your user name and password. * Go to Edit--->Manage attachment.[step 1] * Enter sample_id and click on 'add attachment'.[step 2] * Enter attachment_id , description and file type as shown in below screenshot.Then Browse the file you want to upload that file should be in format that you wrote in file type.and click on 'Insert Attachment'.

nchsls:c:biochemistry:document:authorities_and_responsibilities

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

Use following instructions to give appropriate priviliges to various users during installation / restoration of LIS in failure. How to generate new user ID for LIS Login into the php adminer as shown below. click on cl_general. click on user.

nchsls:c:biochemistry:document:autocalibration_and_manual_calibration_of_zebra_tlp_2844_barcode_printer_and_zebra_gc420t_barcode_printer

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

Autocalibration Zebra_tlp_2844_Barcode printer * Switch off the printer from black switch behind the printer(do not touch main plugged switch). * Press and hold the Feed button and along with it, turn on the black power switch again. * Three barcode lable will come out.

nchsls:c:biochemistry:document:autocalibration_and_manual_calibration_of_zebra_tlp_2844_barcode_printer_and_zebra_technologies_gc420t_barcode_printer

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

nchsls:c:biochemistry:document:autocalibration_and_manual_calibration_of_zebra_tlp_2844_barcode_printer_and_zebra_technologies_ztc_gc420t_barcode_printer

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

nchsls:c:biochemistry:document:autoverification_process

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

VERIFICATION * Login with own ID and Password. * Go to the View button, * Enter the ID from which verification is to be started. * Click on view detail * That report will be shown. * * now this report here is unverified * Click on edit button

nchsls:c:biochemistry:document:autoverification_process_after_editing

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

autoverification process after editing * Open 10.207.3.240 * Enter your user name and password. * click on 'edit'.[Step 1] * Enter sample_Id and click on 'edit_sample'.[figure 2] * In Status, Change from “entered” to “analyzed”. * click on'save_sample'.

nchsls:c:biochemistry:document:b.document

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

On Master page of equipment, write manufacturer’s name, model and serial number or other unique identification for every equipment present in laboratory. This identity is from manufacturer's perspective (Previous identity was from lab perspective)

nchsls:c:biochemistry:document:back_up

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

How to check back up * Open terminal. * Write as shown in image below. *

nchsls:c:biochemistry:document:barcode_or_aliquot_xl-1000

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

Sample preparation/sorting - After centrifugation samples are sorting on basis of total volume and serum/plasma volume for Erba XL 1000 and Erba XL640 * For Plain vacuette * if Total blood volume <2.0, analyses in Erba Xl 640. *

nchsls:c:biochemistry:document:barcode_printer

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

[Common instructions for Zebra] Barcode Printer_Zebra TLP_2844 whole identity of the equipmentBarcode Printer_Zebra TLP_2844 whole_HOSP EQ P-137manufacturer’s name, model and serial number or other unique identificationZebra TLP 2844_41J101401251contact information for the supplier or the manufacturer

nchsls:c:biochemistry:document:big_water_treatment_plant_clinical_lab

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

NEER PLANT 1.MAINTAINENCE SHEET 2.FLOW CHART 3.SOP -This plant is divided into two parts: -A) R.O.PLANT -B) TYPE 1 WATER PLANT -The R.O.Plant is supposed to be operated first.(Left side of the room); -First Check water supply -The inlet valve should be in the position shown as below;

nchsls:c:biochemistry:document:blank_in_erba-xl_640

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

Programme BLANK in ErbaXL-640 * Make one dummy profile named TIRON copied from IRON profile to remove interfernce in first cycle of wash(Keep standard replicates=3 in both profile) * Go to Consumabable and programme as below * * Go to QC/calibration

nchsls:c:biochemistry:document:blood_gas_analysis

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

* Training points for ABG * WDI FOR ABG * Operator's guide * Measuring range of all parameter of instrument * Troubleshooting of ABG instrument * How to prepare Heparinized syringe

nchsls:c:biochemistry:document:bpb_bromophenol_blue_dye

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

Dissolve 10 mg of BPB dye in 10 ml of buffered solution.

nchsls:c:biochemistry:document:buffer_preparation_sop_in-house

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

nchsls:c:biochemistry:document:c.document

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

Maintain contact information for the supplier or the manufacturer of each equipment which will useful when there is breakdown of instrument.Procedure to be followed during breakdown is described in k

nchsls:c:biochemistry:document:cal

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

Calcium examination procedure * Learn operation of Erba XL-640 and Erbachem. * Select equipment * Use Erba XL-640 as peferred equipment. * If Erba XL-640 is malfunctioning and can not be repaired within period acceptable to clinicians, use Erbachem and follow procedure for responding to Erba XL-640 failure

nchsls:c:biochemistry:document:cal_ocpc

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

nchsls:c:biochemistry:document:calcium_arsenazo_reagent

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

How to make calcium reagent [Calcium Reagent]

nchsls:c:biochemistry:document:calculation_of_ldl_cholesterol

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

Calculation Of LDL / VLDL by LIS system automatically * Open 10.207.3.240 * Enter your user name and password. * click on 'edit'.[Step 1] * Enter sample_Id and click on 'edit_sample'.[figure 2] * In Status, Change from “entered” to “analyzed”. * click on'save_sample'.

nchsls:c:biochemistry:document:calibrate_ph_meter

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

INSTRUMENT START UP By pressing the on/off switch,instrument will display “LOAD” message & “x” blinking untill initialization is complete. PH CALIBRATION * The instrument should be recalibrated in following condition: * At least once a week

nchsls:c:biochemistry:document:calibration_and_quality_control_frequency

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

Frequency of calibration and IQC Erba XL-640ParameterCalibrationIQCCalibration PolicyCorrelation FactorEQC Policy calibrator and Replicates/QC and Replicates prepare 200ul calibratuo cups and devide 500ul QC cups in to 200ul perform recalibration of same lot if qc results are out of 1SD on same side for more than 15days

nchsls:c:biochemistry:document:calibration_calculation_and_quality_control_in_erba_xl-640

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

How to calibrate on Erba XL-640How to do Internal quality control on Erba XL-640How to do External quality control on Erba XL-640

nchsls:c:biochemistry:document:calibration_details_in_database

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

How to enter calibration details in database * Open 12.207.3.240. * Enter user name and password. * Go to Quality---->Calibrations.[step 1] * click on 'New calibration'.[step 2] * Fill details as written below: * Code = select from drop down menu * date_of_calibration = select date of calibration

nchsls:c:biochemistry:document:calibrator_and_qc

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

Current Calibration Frequency, IQC and EQC policy Check for the following while using each bottle of quality control or calibrator Check if it is a bottle of QC or calibrator Check the current lot which is in use.Match bottle with it Check the expiry date of lot before using calibrator or QC Assigned value of calibrators and manufacturer's target value for QC material(Mean of all Instruments)

nchsls:c:biochemistry:document:calibrator_and_qc_targets

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

Assigned value of calibrators and manufacturer's target value for QC material Examination[CAL 2(1042UN)][CAL 3(800 UE)][QC 5(1029UN)][QC 8(767UE)]Method to refer to in literatureALB4.302.964.162.97Bromocresol GreenALP215367182375AMP Optimised to IFCC 37 degreeALT3913037132Tris buffer without P5P 37degreeAMY

nchsls:c:biochemistry:document:cbb

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

procedure o prepare CBB staining solution * take 250mg CBB dye in 100ml mehanol * add 25 ml aceic acid * add 125 ml water.

nchsls:c:biochemistry:document:centrifugation

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

centrifugation * Centrifuge fluoride and plain vacutte for 10 minutes at 3000 rpm. How to operate centrifuge Observations: * If sample is highly hemolyzed,lipemic or icteric ,add examination interference in that sample id and write “sample is hemolyzed”. * If sample is highly hemolyzed , add examination rejection in that sample id and write

nchsls:c:biochemistry:document:centrifugation_preparing_aliquot_how_to_perform_examination_in_vitros_3600

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

-Training points for VITROS-3600

nchsls:c:biochemistry:document:centrifugation_xl-1000

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

Centrifugation XL-1000 * precaution during centrifugation of barcoded samples. * vacuette handled carefully in centrifuge. * Do not rotate vacuette after keeping in centrifuge. * Samples must centrifuge for 10 mins. * After centrifugation, if clot have not a flat surface again centrifuge for 10 mins.

nchsls:c:biochemistry:document:centrifuge

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

General Instruction Centrifuge Remi PR-23 HOSP-EQ-P-138-VCDB-4173 Equipment Documents and Recordsidentity of the equipmentCentrifuge Remi PR-23 HOSP-EQ-P-138-VCDB-4173manufacturer’s name, model and serial number or other unique identificationCentrifuge Remi PR-23 VCDB-4173

nchsls:c:biochemistry:document:changing_precolumn_filter

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

Replace the filter in the following cases. * When the filter counter reaches 350 injections. * When the pressure is more than the pressure (which is indicated onthe column inspection report) +4 MPa. Procedure - If the analyzer is not in STAND-BY state, wait for the assay to end and STAND-BY to be displayed.

nchsls:c:biochemistry:document:cho

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

Procedure for Cholesterol examination * Learn operation of Erba XL-640 and Erbachem. * Select equipment * Use Erba XL-640 as preferred equipment. * If Erba XL-640 is malfunctioning, stop the examination and follow procedure for responding to Erba XL-640 failure * Read following documents references [CHO Kit literature of cormay] [CHO Kit literature of meril] Note:

nchsls:c:biochemistry:document:cholinesetase

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

Procedure for Cholinesterase examination *Learn operation of Erba XL-640 and Erbachem. * Select equipment * Use Erba XL-640 as peferred equipment. * If Erba XL-640 is malfunctioning and can not be repaired within period acceptable to clinicians, use Erbachem and follow

nchsls:c:biochemistry:document:ck_total

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

Procedure for CK_Total(creatine kinase-Total) examination * Learn operation of Erba XL-640 and Erbachem. * Select equipment * Use Erba XL-640 as preferred equipment. * If Erba XL-640 is malfunctioning, stop the examination and follow procedure for responding to Erba XL-640 failure * Read following documents

nchsls:c:biochemistry:document:ckmb

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

Procedure for CKMB(creatine kinase-MB) examination * Learn operation of Erba XL-640 and Erbachem. * Select equipment * Use Erba XL-640 as preferred equipment. * If Erba XL-640 is malfunctioning, stop the examination and follow procedure for responding to Erba XL-640 failure * Read following documents references

nchsls:c:biochemistry:document:common_laboratory_informations_related_to_the_examinations

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

^ Common Laboratory Information Related to Examinations ^^ purposeRefer to reference literatureprinciple,methodRefer to reference literatureperformance characteristics-truenessRefer to reference literature. These are characteristic performance found during validation. Refer them to compare laboratory performance of the examination during independent verification of examination

nchsls:c:biochemistry:document:conductivity_meter

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

Equipment Primary Standards * Prepare 10 mmol/L KCl * at 30'c It is 1546 uSi/CM conductivity * Prepare serial dilutions of 150,15 and 1.5 uSi/CM * Reference: [Conductivity Primary Standards]

nchsls:c:biochemistry:document:continuous_dirty_cuvette_messages

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

Following troubles may come * Machine will show dirty cuvette errors continuously. * Machine will show Autospan failed. * Machine will stop working in between the run. * Machine will not start. What to do when above listed troubles occurs ???

nchsls:c:biochemistry:document:creatine

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

Creatinine Procedure for Creatinine measurement nchsls:c:biochemistry:document:examination_procedure:LDHElectroinc-only documentEdition (1) 27-01-2014Electronic copy of the document is stored at NCHSLS server, NCHS, SuratElectronic copy of the document may be printed. However, It should be destroyed as early as possible after use, to ensure that only latest documentation is used every time.

nchsls:c:biochemistry:document:creating_user_in_moodle

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

User name and password in moodle Resident and faculty * User name = dr_(your name) * password = (Your name)(first letter capital)@123 * For example * user name = dr_herat * password = Herat@123 Technician * User name = mr or ms or mrs_(your name)

nchsls:c:biochemistry:document:creatinine

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

Creatinine examination procedure * Learn operation of Erba XL-640 and Erbachem. * Select equipment from the list below. * Use Erba XL-640 as peferred equipment. * If Erba XL-640 is malfunctioning and can not be repaired within period acceptable to clinicians, use Erbachem and follow

nchsls:c:biochemistry:document:creatinine_reagent

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

How to make Creatinine Reagents * Print Label How to make 5gm% Albumin Blank [albumin 5 gm%]

nchsls:c:biochemistry:document:critical_reports

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

How to inform critical Report Automatically * If there is any critical value in report, “critical alert” will be automatically added during verification of reports. * Then technician will inform the critical report to respective person and write his or her name in critical alert remark.

nchsls:c:biochemistry:document:crp

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

---CRP kit literature pdf--- ---CRP Calibration with linearity establishment--- * lyophilized crp bottle is reconstituted with 1 ml DI water, incubated for 10 to 15 minutes with periodic shaking until solutes becomes completely dissolved. * 100 Ul of the mixture is added with 100 Ul of Di water.

nchsls:c:biochemistry:document:current_calibration_frequency_iqc_and_eqc_policy

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

Frequency of calibration and IQC Erba XL-1000 ParameterCalibrationIQCCalibration PolicyCorrelation FactorEQC Policy calibrator and Replicates/QC and Replicates prepare 200ul calibratuo cups and devide 500ul QC cups in to 200ul perform recalibration of same lot if qc results are out of 1SD on same side for more than 15days

nchsls:c:biochemistry:document:current_convention_for_allocation_of_qc_id

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

* Normal range IQC sample is called QC-5 and numbered as 5YYMMDDHH (Nine digits) * Abnormal range IQC sample is called QC-8 and numbered as 8YYMMDDHH (Nine digits)

nchsls:c:biochemistry:document:d.document

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

Enter the date of receiving of each equipment in LIS and also enter the date of service for each equipment.

nchsls:c:biochemistry:document:d_dimer

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

kit literature [coral DDIMER] [Coral DDIMER] [spinreact kit literature] [ SPincreact ddimer Calibrator] D-DIMER Calibration with linearity establishment * lyophilized D-DIMER calibrator bottle is reconstituted with 1 ml(1 gm qc water) DI water, incubated for 10 to 15 minutes with periodic shaking until solutes becomes completely dissolved.

nchsls:c:biochemistry:document:dbil

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

Direct bilirubin examination procedure * Learn operation of Erba XL-640 and Erbachem. * Select equipment * Use Erba XL-640 as peferred equipment. * If Erba XL-640 is malfunctioning and can not be repaired within period acceptable to clinicians, use Erbachem and follow

nchsls:c:biochemistry:document:de-ionizing_water_plant

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

Identity of the equipment Manufacturer’s name, model and serial number or other unique identification Contact information for the supplier or the manufacturer Date of receiving and date of entering into service Location 1.Research Laboratory 2.Clinical Laboratory

nchsls:c:biochemistry:document:detailed_procedure_for_tat

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

nchsls:c:biochemistry:document:details_of_water_treatment_plant_components

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

the first thing that incoming tap water encounters, and as the name implies it filter out sediment. * If sediment filter bypassed , the sediment would then rapidly clog the activated carbon filter, making it much less useful and potentially risking damage to the RO membrane.

nchsls:c:biochemistry:document:dextrose_wash_solution_preparation

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

Dextrose Wash Solution * take 50 ml of 0.1 mol/ L EDTA and make up to 500 ml with 5% dextrose solution. * Or take 50 ml of 0.1 mol/ L EDTA and 25 gm glucose and make upto 500 ml. * Keep it preserve in refrigerator. EDTA Solution calculation {:nchsls:c:biochemistry:document:0.1_molar_edta_solution_counting_.ods|}}

nchsls:c:biochemistry:document:digital_display_thermometer

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

Use calibrated digital display thermometer for recording refrigerator temperature * How to calibrate digital display thermometer * Calibrate all in use digital display every 6 months. * record calibration data in [LIS]

nchsls:c:biochemistry:document:digital_tachometer

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

Digital Tachometer identity of the equipmentDigital Tachometer HOSP-EQP-REG-PG-26 manufacturer’s name, model and serial number or other unique identificationDigital Tachometer-Indi-6121contact information for the supplier or the manufacturerNAdate of receiving and date of entering into service

nchsls:c:biochemistry:document:digital_thermometer

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

Digital Thermometer identity of the equipmentThermometer with sensor Pt-1000-HOSP-EQP-REG-PG-134 manufacturer’s name, model and serial number or other unique identificationDigital Thermometer-Elinco, TTX-483contact information for the supplier or the manufacturer

nchsls:c:biochemistry:document:direct_bilirubin_reagent

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

[Direct Bilirubin Reagent]

nchsls:c:biochemistry:document:dithionate_buffer

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

nchsls:c:biochemistry:document:dithionite_buffer

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

nchsls:c:biochemistry:document:dldl

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

Direct LDL examination procedure * Learn operation of Erba XL-640 and Erbachem. * Select equipment * Use Erba XL-640 as peferred equipment. * If Erba XL-640 is malfunctioning and can not be repaired within period acceptable to clinicians, use Erbachem and follow

nchsls:c:biochemistry:document:dna_extraction_and_dna_electrophoresis

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

[Dna Isolation and Electrophoresis] References [DNA LADDER KIT LITERATURE] [Taq DNA polymersae kit literature]

nchsls:c:biochemistry:document:do_following

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

Procedure for withdrawn report * open 10.207.3.240. * Enter your user name and password. * Go to 'edit' .[Step 1] * Enter sample Id of report on which changes are made.[Step 2] * Click on 'save sample' [Step 3] * Add “Z_Withdrawn_Report” from below menu. * write in following format

nchsls:c:biochemistry:document:document

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

date of entering into service

nchsls:c:biochemistry:document:e.document

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

Enter location of equipment in LIS

nchsls:c:biochemistry:document:electrolyte_reagent

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

How to make Electrolyte pack |Preparation of electrolyte pack(Erba Xl-640)}} Required Glassware * 1000 ml volumetric flask * Funnel * 2 Glass bowl * 500 microliter pipette Procedure * Put Nacl and Kcl in Glass bowl.Put Glass bowl in Hot air oven at 175'c for 1 hour.(To evaporate water present in Nacl and Kcl)

nchsls:c:biochemistry:document:electrolyte_reagent_check

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

When new lot of reagent made it should be checked by comparing it with older lot reagent. * During checking a new lot, take 1.5mlX2 reagent in eppendroff cup and preserve it in assigned box. This reagent will be checked again when next lot is checked.

nchsls:c:biochemistry:document:electrolytes-na_k

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

Electrolyte examination procedure references[Na+ Reference literature 1]environmental and safety controls[MSDS of sodium chloride] [MSDS of Potassium chloride] [MSDS of Lithium carbonate] Preparation of Electrolyte reagent Preparation and use of Electrolyte reagent Filling of Electrolyte pack Maintenance after filling fluid pack * Absurd values for Na & K How To analysed electrolyte in different sample types * Preparation of Aliquots * Write sample id on ependroff cups with per…

nchsls:c:biochemistry:document:electronic_pipette

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

Electronic Pipette User Manual * * *

nchsls:c:biochemistry:document:electrophoreis_power_supply

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

Documents on use of Electrophoresis Power supply[Systronics Electrophoresis power supply -606 manual]Manual for other power supply

nchsls:c:biochemistry:document:elisa_reader

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

Document for ELISA reader [Biorad elisa reader user manual]Elisa reader installation Procedure for taking OD in Elisa reader * Switch on the elisa reader.LCD screen display 'password'.Enter '00000' for password.Again press enter. * Open terminal in computer. * Write command “su” as described in window and give passward for root.

nchsls:c:biochemistry:document:elisa_reader_installation

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

New Instructions for 64 bit * login as root * Download [for 64 bit] * Extract. It will be extracted in a folder called 'elisa' * It may be in /root/Downloads or in /root/ * Start terminal. go to in the elisa folder * example command to go to elisa folder

nchsls:c:biochemistry:document:elisa_washer

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

Documents for Thermoscintific washer [User manual of Thermoscientific washer] Procedure for washing plate * Give prime wash.click prime button on the washer. * Set the number of wells to be washed and put the plate in proper position. * click the command of aspirate/wash and click ok.

nchsls:c:biochemistry:document:emergency_exit_plan

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

Fire exit plans in laboratory [Fire exit plan from centrifuge room][Fire exit plan from store room][Master Fire exit plan][Fire exit plan from store room odg format][Fire exit plan from Centrifugation room odg format]

nchsls:c:biochemistry:document:enter_calibration_in_database

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

How to enter calibration in LIS * Open 10.207.3.240. * Enter user name and password. * Click on Misc----> Tables. * * Following screen will be seen. * * Click on calibration. * Following screen will be seen. * * Click on add blank.

nchsls:c:biochemistry:document:entry_of_sample_into_lis

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

Entry of sample into LIS * Open 12.207.3.240 (In the lab). Open 10.206.10.246 (In the OPD) * Go in New --> New Direct(Transporter). * * Following screen will be seen. * * Fill basic information. * Click on Examination--->BI. * Following screen will be seen.

nchsls:c:biochemistry:document:eppindorf_cups

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

These cups may be labeled as follows: Normal Level: QC-5 Abnormal Level: QC-8 Q refers to QC sample. Second character onward refers to coding given to source of QC.

nchsls:c:biochemistry:document:eqa

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

NEW LOT OF RIQAS(GENERAL CLINICAL CHEMISTRY AND CSF ARRIVE * Read the literature- for proper cycle number and sample number. * Read the start date and end date(last date of sending result) of every sample * Set the reminder 2 days before start date and end date for each sample

nchsls:c:biochemistry:document:eqa_for_vitros_3600_chemianalyzer_tests

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

Technicians and residents are responsible for analyzing EQA samples Residents are responsible for monthly data analysis of EQA(with that of IQC) Teaching staff members are responsible for preparing final action plan for nonconformance How to use Riqas website

nchsls:c:biochemistry:document:equipment_comparison

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

References[CLSI guideline for Equipment comparison] * Follow above mentioned guidelines as far as possible for those examinations done on more than two equipments. * Use commutable calibrators to calibrate different procedure, equipment and methods * Commutable Calibrators: Those calibrators which are designed for use across equipments/methods/procedures.

nchsls:c:biochemistry:document:erba_xl-640

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

^Erba XL 640^ identity of the equipmentErba XL-640 HOSP-EQ-P-10:[at_of_erba_xl_640.pdf]manufacturer’s name, model and serial number or other unique identificationFully Automated Chemical Analyser ERBA XL 640, Sr=640051 ,Modal no;XL-640contact information for the supplier or the manufacturer

nchsls:c:biochemistry:document:erba_xl-640_fluid_managing

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

For Refilling DI Water and Cleaning solution * Remove sensor cap of DI water container behind Erba XL-640. * Empty DI water carba present on platform into DI water container. * close sensor cap of DI water container.(For Cleaning solution add 100 ml CLEANING SOLUTION in 10 L container and fill rest of carba with DI water.)

nchsls:c:biochemistry:document:erba_xl-1000

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

^Equipment (ERBA XL_1000) Documents and Records^^^ RequirementType and LocationHelpa.identity of the equipmentErba XL-1000 HOSP-EQ-P-8:Use Equipment Name and Hospital Register page Number to create ID. It is same as in LIS cl_general.equipment.equipment

nchsls:c:biochemistry:document:erba_xl_1000_software_failure

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

Some important images for important information related to Erba XL_1000

nchsls:c:biochemistry:document:erbachem

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

Documents on use of Erbachem[User manual part 1][User manual part 2]Persons authorised to operate erbachem Erba Chem 5 Plus semiauto 1 HOSP-EQ-P-3-1 identity of the equipmentErba Chem 5 Plus semiauto 1 HOSP-EQ-P-3-1manufacturer’s name, model and serial number or other unique identificationTRANSASIA BIO-MEDICALS Ltd. Erba Chem 5 Plus 060379

nchsls:c:biochemistry:document:examination_process

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

Procedures for Examination ProcessProcedure for selection, verification and validation of examination proceduresProcedures for examination

nchsls:c:biochemistry:document:exchange_of_sample_with_other_laboratories

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

Requirements of various Standards ISO 15189:2012 requirementNABL 112:2019 requirement Documents SOPMoU of NCHS with SMIMERmou_bharuchmou_dangmou_dharampurmou_navasari MJJ Hospitalmou_navasari vasda koteg Hospitalmou_Vyara General Hospitalmou_valsad GMERS medical collegemou_dharampur hospital

nchsls:c:biochemistry:document:eye_wash_facility

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

Eye wash facility * Eye wash kit is present on refrigerator no.17 * Eye wash kit contains 2 NS pint , IV set ,Antibiotic eye drop and contact Eye opd and Dr.Priti(982585894) and Dr.Trupi(9909274365). How to use kit * First remove needle from I.V set.(Needle may injure your eye)

nchsls:c:biochemistry:document:f.document

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

Enter condition of each equipment when received by laboratory either it is new, used or reconditioned etc.

nchsls:c:biochemistry:document:ferritin

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

** Instruction for Ferritin calibrator ** * 3 level of calibrator ,frozen powered form * keep at room temperature for 10-15 min * reconstitute with 1 ml of QC water * Make 5 cup of 200 microliter of each level * 1 cup of each l;evel will be used for calibration

nchsls:c:biochemistry:document:filling_of_electrolyte_pack

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

ISE reagent pack - As shown in pic below ISE reagent pack containing two separate reagent bags of “ transfer bags” for standard A and standard B * take reagent into dispencer of perticular standards (A and B)fron reagent bottle. * Dispense the reagent into ISE reagent bag.

nchsls:c:biochemistry:document:first_aid_kit

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

First Aid facility * First Aid kit is present on refrigerator no.18 * First Aid kit contains 1 NS pint, Bandage, Gauze piece, Sticking band,Cotton and Antiseptic cream. How to use kit * First clean the wound or affected part with NS and Gauze piece.

nchsls:c:biochemistry:document:follow_this_sop_for_randox_reagent

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

1) How to prepare and Aliquot of Cholinesterase Reagent??? * Dissolve R1 in 30 ml and R2 in 1 ml of QC water. * Mix R1 and R2 after 15 min when there is complete dissolving of R1 and R2. * Aliquot 1.5 ml of reagent in plain vaccutte after proper washing of vaccutte.

nchsls:c:biochemistry:document:for_pepsin_wash_in_xl-640_breakdown

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

when there is long time instrumental breakdown fill electrolyte path with pepsin solution every 1 to 2 hourly

nchsls:c:biochemistry:document:free_t3

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

** Instruction for Free T3 calibrator ** * 3 level of calibrator ,frozen powered form * keep at room temperature for 10-15 min * reconstitute with 1 ml of QC water * Make 5 cup of 200 microliter of each level * 1 cup of each l;evel will be used for calibration

nchsls:c:biochemistry:document:free_t4

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

Procedure for Free T4 examination references[Free T4 kit literature]environmental and safety controls[MSDS of Free T4 kit]CentrifugationSample preservation for free T4 How to perform Free T4 examination Schedule for performing TSH and Free T4 examination ** Instruction for Free T4 calibrator ** * 3 level of calibrator ,frozen powered form * keep at room temperature for 10-15 min * reconstitute with 1 ml of QC water

nchsls:c:biochemistry:document:free_t4_examination

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

Preparation before doing procedure for ELISA Preparation before starting free T4 ELISA * Dispense 100uL samples in wells of dummy plate according to worklist using 100 uL pipette. * Take out the microtiter plate. Don't touch the lower surface of the plate. * Dispense 25 uL sample from the dummy plate into the microtiter plate using multichannel pipette.Note the time of starting dispensing of sample.

nchsls:c:biochemistry:document:fsh

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

Instruction for for FSH calibrator * 2 level of calibrator ,ready to use ,2 ml * 10 cup of 200 microliter was made of each calibrator * These cups were labelled ,parafilmed and place in urine microcontainer and labelled as a fresh cup * 1 cup was used for calibration

nchsls:c:biochemistry:document:ftdjk

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

* The interior of the autoclave is filled with water till a defined level. - An empty bucket is kept in the autoclave. * The red bag containing vacuttes , tips, ependroff cups, etc. is kept in the bucket. * Thereafter, autoclave is switched on after placing the lid.

nchsls:c:biochemistry:document:fully_autoclave_vertical_instrument

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

How to operate fully autoclave vertical instrument * AUTOCLAVE MAINTENANCE SHEET * *

nchsls:c:biochemistry:document:g.document

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

As per Manufacturers User Manual

nchsls:c:biochemistry:document:g6pd

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

1) G6PD kit literature

nchsls:c:biochemistry:document:give_permission_to_allow_scanning

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

* Make folder and name it as “scan” at specified location of particular computer in which you want to collect scanned files. * Open terminal and log in as “su”. * Write command as display below to give permission to defined folder. *

nchsls:c:biochemistry:document:glucose

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

Glucose examination procedure * Learn operation of Erba XL-640 and Erbachem. * Select equipment * Use Erba XL-640 as peferred equipment. * If Erba XL-640 is malfunctioning and can not be repaired within period acceptable to clinicians, use Erbachem and follow

nchsls:c:biochemistry:document:glycine-tris_buffer_full_saturated

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

ChemicalQuantityTris base3.0 gmGlycine powder14.0 gm Make up to 1000 ml. Make sure that its pH is 8.6. This is full saturated buffer.

nchsls:c:biochemistry:document:glycine-tris_buffer_half_diluted

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

ChemicalQuantityTris base3.0 gmGlycine powder14.0 gm Make up to 2000 ml. Make sure that its pH is 8.6. This is half diluted buffer.

nchsls:c:biochemistry:document:glycine-trise_buffer

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

ChemicalquantityTris base3.0 gmGlycine 14.0 gm Make up to 1000 ml. Make sure that its pH is 8.6

nchsls:c:biochemistry:document:h.document

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

* Laboratory should have following pre-setup for each equipment. * Proper Space * Proper water Supply * Stable Electrical Supply * Other as per Manufacturers User Manual

nchsls:c:biochemistry:document:haemolysate

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

100 ml Di water + 2 ml Triton x -100 + 20 mg KCN

nchsls:c:biochemistry:document:haemolysate_solution

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

Hemolysate * 90 ml TEB Buffer (Half diluted). * 2 ml Triton x -100 * 20 mg KCN * 8 ml 0.1 M EDTA * Mix well, Preserve it in fridge. Acrylamide Hemolysate * Take 1 gm acrylamide * Add 10 ml hemolysate. * Allow acrylamide to dissolve

nchsls:c:biochemistry:document:half_diluted_teb_buffer

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

* Take approx. 600 ml of DI water in 2 liter flask * Add 10.2 gm Tris base * Add 3.2 gm boric acid * Add 0.2 gm EDTA powder * Mix properly with stirrer and do upto 2 liter * Measure the Ph of final buffer and it would be 8.6

nchsls:c:biochemistry:document:hb_a_hb_f_hb_s_control_s

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

How to prepare HbA ,HBS and HBF control For HBF Control preparation * Collect about 2-4 ml cord blood in plastic bottle containing 2 ml CPDA * Alternatively add washed RBC of new born EDTA sample in 2 ml CPDA bottle till total volume become 4 ml approximately. Preserve at 2-8'C till required amount is collected.

nchsls:c:biochemistry:document:hba1c

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

Genaral Instruction / Specific instruction to use HPLC (HLC-723GX) in the laboratory * How to start Instrument * How to analyze sample * How to change buffer solution and wash solution * how to stick barcode for HbA1c * changing precolumn filter * SOP of barcode setting * INSTRUCTION BEFORE RUN SAMPLE * * * * * * * * * * * * Buffer preparation SOP (In-house)

nchsls:c:biochemistry:document:hbv_vaccivation

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

Procedure * When new staff joins the laboratory, ask about vaccination status * If vaccination is done, ask about number of doses taken, tentative date and month of vaccination * Enter the datails in LIS * If vaccination is not taken, give first dose of HBV and enter in LIS.

nchsls:c:biochemistry:document:hcl_reagent

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

* Take 500 ml Di water in 1 liter volumetric flask * Take 20 ml concentrated HCL in 20 ml Volumetric flask * Add in 500 ml DI water * Do upto 1 liter and mix well

nchsls:c:biochemistry:document:hdl_cholesterol

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

HDL cholesterol examination procedure * Learn operation of Erba XL-640 and Erbachem. * Select equipment * Use Erba XL-640 as peferred equipment. * If Erba XL-640 is malfunctioning and can not be repaired within period acceptable to clinicians, use Erbachem and follow

nchsls:c:biochemistry:document:hemoglobin_electrophoresis_and_dithionite_test-_modified

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

Hemoglobin electrophoresis and Dithionite test examination procedure * Learn operation of Electrophoresis tank and Electrophoreis Power supply. Electrophoresis tank Electrophoreis Power supply * Read following documents References[Hemoglobin reference literature]environmental and safety controls [MSDS of agarose]

nchsls:c:biochemistry:document:hemoglobin_electrophoresis_and_dithionite_test

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

Hemoglobin electrophoresis and Dithionite test-(use this) examination procedure * Learn operation of Electrophoresis tank and Electrophoreis Power supply. * Read following documents References[Hemoglobin reference literature]environmental and safety controls [MSDS of agarose] [MSDS of Amido black] [MSDS of boric acid] [MSDS of KCN] [MSDS of triton X] [MSDS of Tris base] [MSDS of EDTA] [MSDS of acetic acid] Introduction Electrophoresis is a comprehensive term that refers to the migration o…

nchsls:c:biochemistry:document:hippurate_buffer_ph_8.8

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

ChemicalQuantityTris base7.5 gmNa hippurate(base)10 gmHippuric acid2.25gm * Add Tris base and Na hippurate into 900 ml Di water. * Add hipuric acid in that solution. * Check pH,it should be 8.8 * Make up to 1000 ml. * Check pH again.pH 8.8 should be final.

nchsls:c:biochemistry:document:hostel

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

girls hostel room no 001

nchsls:c:biochemistry:document:how_to_add_new_tests_in_riqas_program

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

* Go to the provided link * Following screen will be seen * Do Login with username- biochemistrygmcs@gmail.com * Following screen will be seen * Go to data entry---> assay details. * Following screen will be seen * Click on instrument in which you want to add new parameter.

nchsls:c:biochemistry:document:how_to_calibrate

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

* Note temperature and humidity of room temperature * Clean rettech weighing scale properly * Take calibrated sartorious weight 10 gram and Maruti weight 1 kg And 2 kg. * weight one of the weight, take reading and bring it back to 0.repeare same for all weight.

nchsls:c:biochemistry:document:how_to_calibrate_digital_display_thermometer

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

Use externally calibrated Digital thermometer to calibrate digital display thermometer. * For 2-8 degree * keep the beaker full of water in refrigerator 24 hours before the calibration. * At the time of calibration, keep all the thermometer along with calibrated thermometer inside the the beaker.

nchsls:c:biochemistry:document:how_to_calibrate_on_erba_xl-640

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

How to calibrate on Erba XL-640 * Analyze one sample after making new reagent,before calibration in order to check reagent blank and any gross mistake. * Current calibration frequency policy * prepare Randox cal 2 and cal 3 * Make new profile of parameter you want to calibrate in erba XL-640.. * Changes to make in new profile as shown in screenshot by click on 'edit' button.

nchsls:c:biochemistry:document:how_to_change_lamp

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

steps for changing lamp * In one excel sheet, note down current gain of all filters of ErbaXL 640 from maintenance menu. * Take out sealed unused lamp from Preventive Maintenance kit of ErbaXL 640. * Keep hands away from glass part of new halogen lamp. Make sure that there is no crack or breakage in the glass part.

nchsls:c:biochemistry:document:how_to_change_opd_ip_adderess

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

HOW TO CHANGE OPD IP ADRESS STEP-1 Do right click on NETWORK ICON present on right upper corner of computer screen shown below. Click on “EDIT CONNECTIONS” STEP-2 One table of “NETWORK CONNECTIONS” will open. Click on “WIRED CONNECTION 1

nchsls:c:biochemistry:document:how_to_dilute_sample_manually_and_calculate_result

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

nchsls:c:biochemistry:document:how_to_do_entry_of_deleted_sample_with_same_sample_id

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

How to do entry if sample is deleted * Open adminer * Go to primary result * search sample id which was deleted * Add MRD and sample requirement in that sample id * Code of MRD is 1000 and sample requirement is 1001 * Now go to LIS * MRD and sample type has been added

nchsls:c:biochemistry:document:how_to_do_external_quality_control_on_erba_xl-640

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

nchsls:c:biochemistry:document:how_to_do_internal_quality_control_on_erba_xl-640

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

How to do quality control on Erba XL-640 Preparation of quality control sample(Randox) * Take 5 ml Qc water(DI water of 0.00 microsiemens/cm) in volumetric flask. * Gently transfer water in Randox Human assay level 2 and level 3 bottle.Pour last drop of water by simply rotate flask over edge of bottle.Do not beat the flask over bottle.

nchsls:c:biochemistry:document:how_to_get_numbers_of_copies

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

click on User Tools shown below by pointing with pen. click on down arrow click on “counter” button you will get numbers of copies like shown below. if you want to print it out then click on “print counter list” shown below

nchsls:c:biochemistry:document:how_to_install_erba_xl_software_in_computer

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

* open command prompt. * login into root. * password is- proteo#####s * * while giving backup, first delete the previous database. so click on check database. then click on delete database. then after click on the browse file. give bkp file first. (with extention .bkp) then it will ask for blank database. give BKP file. (with .BKP extention).

nchsls:c:biochemistry:document:how_to_install_new_toner_in_printer

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

1.take new toner from kit. 2.remove its cap. 3.open the front door of printer. 4.pull green coloured square shaped part in outward direction.. 5.after upward pulling of that upper green part,press lower green part showing in picture. 6.pull gently this green part outward so that old toner will appear. Gently rotate it outward(anti-clockwise) and take old toner out. then replace new toner and rotate it inward(clockwise) and fix it.

nchsls:c:biochemistry:document:how_to_install_windows_in_computer

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

* Run the windows pendrive. * switch on the computer. * continue press the F10 for boot the computer. * follow the steps. * * * * * * * * * * * * * *

nchsls:c:biochemistry:document:how_to_make_cup_for_qaf_sample

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

Preparation of Aliquot * QAF urine sample first keep at room temperature then make 500 microliter eppendroff cup prafilmed and label it ,then store in deep fridge. * QAF serum sample first keep at room temperature then make 500 microliter eppendroff cup prafilmed and label it ,then store in deep fridge.

nchsls:c:biochemistry:document:how_to_make_d-dimer_internal_quality_control

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

* We Have ready made, lyophilized, dry powder formed calibrator with two different concentration of D-DIMER. See photograph * 3200 ng/ml * 5420 ng/ml * We are using Normal Saline (NS) for reconstitution purpose. * For reconstitute the Calibrator with 3200 ng/ml concentration, add 1 ML of NS into dry calibrator. So resulted concentration will be of 3200 ng/ml.

nchsls:c:biochemistry:document:how_to_make_qaf_six_monthly_file_as_record

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

RAW DATA OF QA FORUM QA Forum Tests results will be saved as ods file as mentioned here QA FORUM SIX MONTHLY FILE AS RECORD

nchsls:c:biochemistry:document:how_to_mix_sample_before_analysis

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

nchsls:c:biochemistry:document:how_to_operate_centrifuge

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

Do in house of calibration of centrifuge every 6 monthly How To Operate Remi Centrifuge PR 23 [User manual of PR-23]It was seen that During centrifugation vacuttee cap touches rotor of centrifuge while it is in horizontal position so it prevent the bucket to come in vertical position.to prevent this following should be done.during cotton filling equal amount of cotton should be fill in both the rotor(opposite one)in same position.see below picture:

nchsls:c:biochemistry:document:how_to_operate_fully_autoclave_vertical_instrument

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

* Tightly press over part as shown in below image so that top cover is movable and can be open and close easily. * Fill the Deionized water only upto mark as shown in below image * Put sample bag (Red bag) in bucket as shown in below image .As there are two bucket for sample,use two in case of more samples are there.

nchsls:c:biochemistry:document:how_to_perform_examination_in_erba_xl-640

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

Note: Following instruction is useful only if operator is familiar with Erba XL-640 Program Erba XL-640 for the examination.[sorted test parameters][exported test parameters] Current calibration frequency policy Important notesAbnormal Alert and Reference RangeManagement of reagent aliquotloading reagents in Erba XL-640CalibrationInternal Quality ControlEQA * Run samples in Erba XL-640 * Export results from Erba XL-640 * Import results in LIS Preparation of Aliquots * Write sample i…

nchsls:c:biochemistry:document:how_to_perform_external_quality_control_on_erba_xl-640

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

Procedure to reconstitute EQAS * EQAS sample will be reconstituted same as Internal Quality Control * As we have to perform many tests with the EQAS sample, we will have to divide it into many parts and preserve it. * 4 cups of 500 microlitre. ( to be done with morning QC for four consecutive days )

nchsls:c:biochemistry:document:how_to_perform_page

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

- parts of PAGE apparatus - two slides are used for gel preparation. put back slide first as shown in figure - we take 2 spacer in between 2 slides. So gel will be of spacer thickness. to make air tight contact of slides and spacer we use veseline. Apply veseline in both the sides of spacer

nchsls:c:biochemistry:document:how_to_prepare_heparinized_syringe

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

Reference-

nchsls:c:biochemistry:document:how_to_print_label_for_reagent_bottles

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

Print label from LIS * Open 12.207.3.240 * Enter your user name and password. * Click on Misc-----> Label. * * Following screen will be seen. * * Click on sample tube barcode. * Following screen will be seen. * * Write sample IDs for which you want to print barcode.

nchsls:c:biochemistry:document:how_to_process_citrated_plasma_qaf_sample

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

From CITRATED PLASMA QAF Sample, following test is performed. * D-DIMER (D Dimer)

nchsls:c:biochemistry:document:how_to_process_edta_qaf_sample

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

From EDTA QAF Sample, following tests will be performed. * Hemoglobin Electrophorosis (Hemoglobin electrophoresis and Dithionite Test (In Use)) * HBA1C (HbA1C)

nchsls:c:biochemistry:document:how_to_process_fluid_rather_than_csf_qaf_sample

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

From FLUID (OTHER THAN CSF) QAF Sample, following tests are performed. * ADA ( ADA in house)

nchsls:c:biochemistry:document:how_to_process_qaf_sample

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

How to process **EDTA** QAF SAMPLE How to process **SERUM** QAF SAMPLE How to process **CITRATED PLASMA** QAF SAMPLE How to process **FLUID(RATHER THAN CSF)** QAF SAMPLE How to process **URINE BIOCHEM** QAF SAMPLE

nchsls:c:biochemistry:document:how_to_process_serum_qaf_sample

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

From SERUM QAF Sample, following tests are performed. * ACETONE (Ketone bodies) * TROP I (hs Troponin-I) * CHOLINEESTERASE (CHE) * PROTEIN ELECTROHORESIS (Serum protein electrophoresis) * PRO-CALCITONIN (Procalcitonin) * 25 OH VIT D (25 OH VIT D) * CRP (CRP)

nchsls:c:biochemistry:document:how_to_process_urine_biochem_qaf_sample

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

From URINE BIOCHEM QAF Sample, following tests are performed. * URINE CALCIUM (Same as serum calcium) CAL (ARSENAZO) * URINE CREATININE (Same as serum creatinine) (Creatinine) * URINE MICRO-ALBUMIN

nchsls:c:biochemistry:document:how_to_remove_air_from_sample_before_analysis

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

Gently mix sample between palm 4 to 5 times keep the sample vertical at 90 degree position with needle with cap kept as shown in below image Gently push from below to remove air completely from sample and extra blood will store in cap of needle as shown in below image

nchsls:c:biochemistry:document:how_to_send_qaf_results

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

HOW TO SEND QAF RESULTS??? * Open the link: * Following window will be seen. * Enter User ID : 8913 * Password: *** * Following window will be seen. * Click on RESULT----> RESULT SUBMISSION * Click on QAF-EQAS-ML-Biochemistry

nchsls:c:biochemistry:document:how_to_set_host_connection_of_instrument_with_lis

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

* open terminal * write ssh root@12.207.3.240 * give password * write ls and then press enter. * so many files will be shown. * Check rp500 in that list. * write cd rp500 * rp500 will be seen at left side. * write ls * so many files will be shown.

nchsls:c:biochemistry:document:how_to_stick_barcode

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

barcode sicking - stick barcode with its level align with the EDTA vaccute sticker. - Barcode should be too low or too high stuck. - It should be proper vertically stuck. -

nchsls:c:biochemistry:document:how_to_use_riqas_website

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

Details of EQC program=== * Open “www.riqas.net” website from computer on which internet available. * Following window open.[step 1] * Click on login.Enter user name and password.User name = biochemisty password=??? * Following window opens.

nchsls:c:biochemistry:document:hs_troponin-i

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

Special instruction for hs Tn-I calibrator * 3 level of calibrator level-1 , level-2, and level-3 * It comes in frozen state and ready to use form * Each level contain 2 ml of calibrator * From main bottle 4 cup was made of 0.5 ml from each level

nchsls:c:biochemistry:document:human_resource_management_process

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

Procedure to runhuman resource management processProcedure for personnel management

nchsls:c:biochemistry:document:hypocloride_daily_wash

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

Procedure for Daily Hypochloride wash * In the morning,before daily maintainence, this wash has to be done. * Fill fresh Hypochloride in both R1(54 position ) & R2(56 position)bottles in Reagent tray. * Keep a cup filled with Hypochloride as a sample in sample tray position No-1.

nchsls:c:biochemistry:document:ibil

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

Calculation Of Indirect bilirubin by manually * Formula for Indirect bilirubin(mg/dl)=Total bilirubin(mg/dl)-Direct bilirubin(mg/dl) Calculation Of Indirect bilirubin by automatically * If report is not verified, perform autoverification process. * If report is verified before calculation, perform

nchsls:c:biochemistry:document:immediate_corrective_action_in_troubleshoot_xl-1000

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

abc

nchsls:c:biochemistry:document:important_intercom_and_mobile_numbers

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

Important intercom number DepartmentIntercom numberDepartmentIntercom numberDepartmentIntercom numberDean373Biochemisty lab366, 317Store section (college)353PA to Dean372Biochem research lab368Store section(Dr. Ketan)488PA to Medical superintendent337

nchsls:c:biochemistry:document:important_notes

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

It was observed that sometimes when TBIL and DBIL levels are very high in sample, it interfers with ALP results as it is measured at 415nm filter. In such sample though ALP level is normal reaction absorbance reaches beyond Abs Lim and it repeat the sample with dilution and gives wrong results. When such sample is diluted mannually, ALP result comes within normal range.

nchsls:c:biochemistry:document:important_notes_regarding_linearity_limit

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

ALT test parameter in ERBA XL 640 for linearity limit% feed 5% so in higher range ALT if graph is not linear machin will take dilution and give result. [ALT linearity limit 5%|]

nchsls:c:biochemistry:document:information_resource_management_process

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

Procedure to run information resource management processProcedure for laboratory information management

nchsls:c:biochemistry:document:insert_qc_comment

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

How to insert Qc comment * Open 12.207.3.240. * Enter user name and password. * Click on QC. * Click on LJ Chart. * Click on Today. * Click on Show Today's LJ. * Following screen will be shown. * * Click on sample ID which is violating any rule.

nchsls:c:biochemistry:document:install_apache_mysql_and_php5

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

How to install apache 2 * Open Synaptic package manager.(Application menu--->Settings--->Synaptic package manager) * Synaptic package manager main window shown in below screenshot. * Click on settings--->Repositories to connect with debian server and download all packages.

nchsls:c:biochemistry:document:install_dokuwiki_documentation

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

How to install Dokuwiki * Login as “root” in server computer where you want to install dokuwiki. * Install Apache, MySQL and PHP5 in computer where to install dokuwiki. * Extract “dokuwiki.zip” file into “var--->www--->http” * Now give permission to dokuwiki as shown in below screenshot.

nchsls:c:biochemistry:document:install_erba_xl-640_software

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

How to install Multi XL software * Insert CD ----> Open CD ----> open “Multi XL-640” folder ----> “set up” folder ----> from set up ,less KB file is selected ----> double click on that file ----> click 'next' ----> “Installation is completed successfully

nchsls:c:biochemistry:document:install_linux

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

Install Debian operating system * Insert bootable CD or USB of Debian in computer and restart the computer. * 'Press any key to boot from cd' such message appear on screen. * Enter on Live(486). * On desktop, icon “install debian wheezy”

nchsls:c:biochemistry:document:install_moodle_e-learning_program

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

Creating user in moodle

nchsls:c:biochemistry:document:install_supporting_softwares_in_windows_xp

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

Install Apache 2.2 ,Mozilla firefox and deskpin softwares in windows * To install apache 2.2 server Insert driver CD in PC ----> open “Apache set up file” ----> give next until installation is completed ----> go to “C:” ----> program file

nchsls:c:biochemistry:document:install_vsftpd_software_from_synaptic_package_manager

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

* Open Synaptic package manager. * Click on “search” or do ctrl + F. * Search menu appear. * Write vsftpd in search menu. * Do right click on vsftpd as appear in below image. * Click on “mark for installation” and Click on

nchsls:c:biochemistry:document:install_windows_xp

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

How to install windows XP * Insert CD of windows XP in cd drive in computer and restart the computer. * 'Press any key to boot from cd' such message appear on screen. * Wait till the partition window appear on screen. * For deleting previous partition , click on that partition,first click

nchsls:c:biochemistry:document:installation_and_troubleshooting_of_ricoh_aficio_mp_2000

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

Installation of Ricoh aficio MP 2000 * To download ppd file click on [Ricoh ppd file] * First download above file & rename as ricoh-aficio-mp-2000.ppd (by removing .pdf extension),then browse this file. * In Debian 10 onwards, Ricoh Aficio MP 2000 - CUPS+Gutenprint v5.2.11 ppd available in installation and openprinting.org works well

nchsls:c:biochemistry:document:installation_and_troubleshooting_of_ricoh_mp_2001l

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

Installation of MP_2001L * Download the file “[Raster]” * Do following in terminal as shown in screen shot below.[step 1] * Open . * Go to administration * Go to Add printer option * It will ask for user name and password * Username is: root then enter the password and click ok

nchsls:c:biochemistry:document:installation_and_troubleshooting_of_tsc_p200_barcode_printer

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

How to install TSC P200 * 1.open . * 2. * 3.Click on “Administration” as shown in above screenshot and after clicking below screen appears. * 4. * 5.Click on “Find new printer” as shown in above screenshot and after clicking below screen appears.

nchsls:c:biochemistry:document:installation_and_troubleshooting_of_tsc_te244_barcode_printer

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

HOW TO CALIBRATE PRINTER BARCODE * * First put finger on start key and press,then switch on from back side of barcode printer then first greeen light will blink followed by light orange,red and last green light will blink. * Press till green light conststant will appearthen release

nchsls:c:biochemistry:document:installation_and_troubleshooting_of_zebra_gc420t_barcode_printer

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

How to install Zebra_Technologies_ZTC_GC420t * open . * * Click on “Administration” as shown in above screenshot and after clicking below screen appears. * * Click on “Find new printer” as shown in above screenshot and after clicking below screen appears.

nchsls:c:biochemistry:document:installation_and_troubleshooting_of_zebra_technologies_ztc_gc420t_barcode_printer

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

nchsls:c:biochemistry:document:installation_and_troubleshooting_of_zebra_tlp_2844_barcode_printer

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

How to install Zebra TLP2844 * open . * * Click on “Administration” as shown in above screenshot and after clicking below screen appears. * * Click on “Find new printer” as shown in above screenshot and after clicking below screen appears.

nchsls:c:biochemistry:document:instruction_before_run_sample

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

INSTRUCTION BEFORE RUN SAMPLE * First before run sample bring stored sample from refrigerator * Do not shake sample * see for PCV part ,if it is below 50 % then remove supernatant part as shown in below image, keep some supernatant part for mixing

nchsls:c:biochemistry:document:instruction_for_csf_eqc_sample

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

* Run CSF Na as urine * Run CSF glucose and MPR as a serum

nchsls:c:biochemistry:document:instruction_for_reagent_tray_adaptor_management

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

* When reagent will be removed from reagent tray along with that also remove adaptor * Do not keep any extra adaptor in reagent tray * If any extra adapter will be there, than it may fall in reagent tray as shown in image below and machine will show error message like

nchsls:c:biochemistry:document:instruction_for_report

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

* When in abg report show .....? this type of sign in any parameter then advice for repeat sample * When in abg report show inreasing arrow sign or decreasing arrow sign then refer the range as shown in below image *

nchsls:c:biochemistry:document:instruction_for_result

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

* When in abg report result show .....? it sujest out of measurent range advice for repeat sample * when in abg report result show inreasing arrow sign or decreasing arrow sign then refer the range as dhoen in below image *

nchsls:c:biochemistry:document:intereference_by_god_pod_glucose_reagent

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

Conclusion * Glucose reagent interferes with TBil estimation * Probably there is reagent carry over. * Introduce one Deteregent wash cycle in between Glucose (interfering test) and Tbil (Interfered Test) in ERBA 640 XL. * Documentation for introducing Wash Cycle

nchsls:c:biochemistry:document:interference

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

Observe Following things in sample: * Hemolysis * Lipemia * Gross Icterus Add Entry of “Examination Interference” from scope menu * Open 12.207.3.240 * Enter your user name and password. * Go to 'view' . * Enter sample Id for which remark has to be added.

nchsls:c:biochemistry:document:interference_of_cholesterol_reagent_in_tg_estimation

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

Conclusion * Cholesterol reagent interferes with TG estimation * Probably there is reagent carry over. * Introduce four Deteregent wash cycle in between Cholesterol(interfering test) and TG(Interfered Test) in ERBA 640 XL. * Documentation for introducing Wash Cycle

nchsls:c:biochemistry:document:interference_of_tp_reagent_in_cal_estimation

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

Conclusion * Total Protein reagent interferes with Calcium estimation * Probably there is reagent carry over. * Introduce four Deteregent wash cycle in between total protein(interfering test) and Calcium (Interfered Test) in ERBA 640 XL. * Documentation for introducing Wash Cycle

nchsls:c:biochemistry:document:interlaboratory_comparison

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

-EQA -Exchange of sample with other laboratories

nchsls:c:biochemistry:document:interlaboratory_comparisons

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

Participation * Refer to LIS->tables->scope for interlaboratory comparison strategy used for individual examinations Login to LIS * In general one of the following is used * RIQAS * QAForum * exchange of samples with other laboratories Monitoring The laboratory monitors the results of the interlaboratory comparison programme(s) and participate in the implementation of corrective actions when predetermined performance criteria are not fulfilled. NOTE: The laboratory participates in…

nchsls:c:biochemistry:document:interleukin-6

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

SOP of IL-6

nchsls:c:biochemistry:document:internal_quality_control

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

Selection of internal quality control material * Purchase internal quality control material from commercial sources with following quality * Select quality control materials that react to the examining system in a manner as close as possible to patient samples.

nchsls:c:biochemistry:document:inventory_management

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

How to make entry of new kit or reagent * Open 12.207.3.240. * Enter user name and password. * Go to Misc---->Reagents. * * Following screen will be shown to you. * * To enter new reagent or new kit, click on Reagent received. * Following screen will be shown to you.

nchsls:c:biochemistry:document:inword_iptables

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

root@localhost:/home/biochemistry# iptables-save # Generated by iptables-save v1.4.21 on Sat Jun 16 11:11:57 2018 *filter :INPUT ACCEPT [815845:715169366] :FORWARD ACCEPT [451:141238] :OUTPUT ACCEPT [491735:668005640] COMMIT # Completed on Sat Jun 16 11:11:57 2018 # Generated by iptables-save v1.4.21 on Sat Jun 16 11:11:57 2018 *nat :PREROUTING ACCEPT [64566:15876100] :INPUT ACCEPT [8540:1091451] :OUTPUT ACCEPT [5030:315868] :POSTROUTING ACCEPT [4899:308719] -A PREROUTING -i eth1 -p tcp -m tcp…

nchsls:c:biochemistry:document:iqc_of_vitros_3600

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

How to make serum pool for IQC of vitros 3600 * First prepare serum pool of two level normal and abnormal of 50 ml in 50 ml plastic wash bottle * Kept at room temperature for at least 2 hr * Mix throughly and filled it in washed plain vaccutte for centrifugation

nchsls:c:biochemistry:document:iron

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

IRON examination procedure * Learn operation of Erba XL-640 and Erbachem. * Select equipment * Use Erba XL-640 as peferred equipment. * If Erba XL-640 is malfunctioning and can not be repaired within period acceptable to clinicians, use Erbachem and follow procedure for responding to Erba XL-640 failure *

nchsls:c:biochemistry:document:iron_tibc

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

IRON + TIBC examination procedure * Learn operation of Erba XL-640 and Erbachem. * Select equipment * Use Erba XL-640 as peferred equipment. * If Erba XL-640 is malfunctioning and can not be repaired within period acceptable to clinicians, use Erbachem and follow

nchsls:c:biochemistry:document:iron_uibc

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

nchsls:c:biochemistry:document:iso_15189_requirement_for_ilse

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

Requirements of various Standards ISO 15189:2012 requirementNABL 112:2019 requirement Documents SOPmou_bharuchmou_dangmou_dharampurmou_navasari MJJ Hospitalmou_navasari vasda koteg Hospitalmou_Vyara General Hospitalmou_valsad GMERS medical collegemou_dharampur hospital

nchsls:c:biochemistry:document:k

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

Potassium examination procedure * Learn operation of Erba XL-640 and Erbachem. * Select equipment * Use Erba XL-640 as peferred equipment. * If Erba XL-640 is malfunctioning and can not be repaired within period acceptable to clinicians, use Erbachem and follow

nchsls:c:biochemistry:document:ketone_bodies

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

Ketones Reference Preparation of Rothera's power * Weight following chemical & mix thoroughly * Sodium carbonate = 20 gm * Ammonium sulfate = 20 gm * Sodium nitroprusside = 1 gm (make fine powder of it with mortar and pastle before mixing)

nchsls:c:biochemistry:document:ketones

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

nchsls:c:biochemistry:document:kit_literature_of_all_test

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

* [kit literature of total Vit-D ] * [kit literature of TSH ] * [kit literature of Troponin ES ] * [kit literature of Testosterone ] * [kit literature of prolactin ] * [kit literature of Vit-D ] * [kit literature of PCT ] * [kit literature of NT-BNP ]

nchsls:c:biochemistry:document:kit_literature_of_hs_trop_i_test

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

[kit literature of Troponon-I ]

nchsls:c:biochemistry:document:labotech_electrophoresis_power_supply_and_apparatus

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

Equipment (Electrophoresis apparatus) Documents and RecordsRequirementType and LocationHelpa.identity of the equipmentAgarose gel electrophoresis appratus with power supply pack HOSP-EQ-P-62-??????:Use Equipment Name and Hospital Register page Number to create ID. It is same as in LIS cl_general.equipment.equipment

nchsls:c:biochemistry:document:ldh

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

Procedure for LDH examination * Learn operation of Erba XL-640 and Erbachem. * Select equipment * Use Erba XL-640 as preferred equipment. * If Erba XL-640 is malfunctioning, stop the examination and follow procedure for responding to Erba XL-640 failure * Read following documents references[LDH Reference literature 1] [LDH Reference literature 2] [LDH Reference literature 3]environmental and safety controls

nchsls:c:biochemistry:document:ldh_reagent

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

nchsls:c:biochemistry:document:ldl_cholesterol

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

LDL cholesterol examination procedure * Learn operation of Erba XL-640 and Erbachem. * Select equipment * Use Erba XL-640 as peferred equipment. * If Erba XL-640 is malfunctioning and can not be repaired within period acceptable to clinicians, use Erbachem and follow

nchsls:c:biochemistry:document:lh

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

Procedure for LH examination * 2 level of calibrator ,ready to use ,2 ml * 10 cup of 200 microliter was made of each calibrator * These cups were labelled ,parafilmed and place in urine microcontainer and labelled as a fresh cup * 1 cup was used for calibration

nchsls:c:biochemistry:document:li

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

Lithium examination procedure * Learn operation of Erba XL-640 and Erbachem. * Select equipment * Use Erba XL-640 as peferred equipment. * If Erba XL-640 is malfunctioning and can not be repaired within period acceptable to clinicians, use Erbachem and follow

nchsls:c:biochemistry:document:lipase

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

Lipase examination procedure * Learn operation of Erba XL-640 and Erbachem. * Select equipment * Use Erba XL-640 as peferred equipment. * If Erba XL-640 is malfunctioning and can not be repaired within period acceptable to clinicians, use Erbachem and follow procedure for responding to Erba XL-640 failure *

nchsls:c:biochemistry:document:lipase_reagent

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

How to make Lipase reagent

nchsls:c:biochemistry:document:lis_calibration_entry

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

nchsls:c:biochemistry:document:lis_creating_users

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

How to create user in LIS * Open 10.207.3.240/phpmyadmin. * Following window is seen.[step 1] * Now click on 'privileges'.[step 2] * If you want to create resident as user , then click on 'edit privilage' in already created resident user.[step 3] * Enter the name of user in User name and enter password.Click on 'Go'.

nchsls:c:biochemistry:document:lis_export_result_from_erba_xl_640

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

How to export result from erba XL-640 * Click on erba XL-640 icon on desktop of pc. * It will ask for username and password. * Username=tech and password=???? * Click on 'Reports(f9)--->Result reprint' in Erba XL-640 menu.[step 1] * Click on 'show' Following window will shown.

nchsls:c:biochemistry:document:lis_how_to_enter_and_edit_request

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

How to enter request * Open 12.207.3.240 * Enter your user name and password. * Click on 'New'.[figure 1] * Enter sample_Id and click on 'new_sample'.[figure 2] * Enter patient deatails properly and click on 'save_sample'.[figure 3] * To add requested test directly click on profile or click on 'id' to add test.

nchsls:c:biochemistry:document:lis_how_to_update_and_reset_sample_id

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

There are two methods Current method * step 1 go to drop down menu as shown below -step 2 go to the monthly id page and enter the date of the current year and month as shown below * step 3 in this step, it will remind you of your procedure as shown below

nchsls:c:biochemistry:document:lis_import_qc_result

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

How to import QC in LIS * There is automation for import the QC results in LIS from instruments. * so no need of importation of QC results. How to import QC in LIS in failure of interface * Open 12.207.3.240. * Enter user name and password.

nchsls:c:biochemistry:document:lis_import_result_from_erba_xl_640

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

How to import Results in LIS * Open 12.207.3.240. * Enter user name and password. * Click on Biochemistry. * Following screen will be seen. * * Click on instrument name from which you want to import test results. For Example, “Import from XL-640

nchsls:c:biochemistry:document:lis_schedule_reminder

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

* Open 12.207.3.240 . * Enter user name and password. * Only residents and faculty can create reminders. * go to the misc. * click on tables. * click on dementia * click on add blank to add a new reminder. * click on pencil icon. * Fill the information in line of Minutes, Hours, DayM, Month, DayW, Text as per needed.

nchsls:c:biochemistry:document:lis_upload_dokuwiki_media

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

How to upload in dokuwiki * Open 12.207.3.241/dokuwiki.[step1] * Click on 'media manager'.[step 2] * Click on 'upload'.[step 3] * Click on 'select files'. Then select the file you want to upload and click on “upload”.

nchsls:c:biochemistry:document:list_of_examination_procedures

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

Examination Procedures * Glucose * ALT * LDH * AMY * UREA * AST * CHO * CKMB * CK_TOTAL * TG * UA * ALP(older) * ALP(CURRENT USE) * PHOSPHORUS * IRON * UIBC * LIPASE * CAL(ARSENAZO) * CAL(OCPC) * MPR * Creatinine * TBIL * DBIL * IBIL * Albumin * Total protein * HDL cholesterol * LDL Cholesterol * VLDL cholesterol * TSH * DLDL * CHE * ADA in house * Li * Free T4 * Hemoglobin electrophoresis and Dithionite Test (In Use) * Hemoglobin electrophoresis and…

nchsls:c:biochemistry:document:list_qc_entry_manual

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

How to insert Qc manually in LIS * Open 12.207.3.240. * Enter user name and password.[step 1] * Click on Quality---->LJ chart insert/comment Qc.[step 2] * Select Date , Equipment name and Examination and write value in QC-normal and QC-abnormal and click on

nchsls:c:biochemistry:document:list_result_entry_manual

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

How to enter sample result manually * Open 12.207.3.240 * Enter your user name and password. * click on 'edit'.[Step 1] * Enter sample_Id and click on 'edit_sample'.[figure 2] * Click on 'save_sample'. Enter examination result and click on 'save_examination'.[figure 3]

nchsls:c:biochemistry:document:loading_buffer

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

Preparation of sample loading buffer * Take 10 ml of glycerol * Add 25 ml of 0.1M EDTA, pH 6.8 * Add 1.21gm Tris base * Add 6ml of 10% HCl * Make up to 90 ml apporx. with DI water. * Check pH, Adjust to 6.8 if required with 10% HCL * Make up to 100ml.

nchsls:c:biochemistry:document:loading_reagents_in_erba_xl-640

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

Reagent Handling * Following steps will be done by user with prior training with Erba XL-640. * Perform reagent refilling once every morning and in the afternoon and whenever required. * Make sure that No RUN is going on * Switch off power at the right side of XL-640 (light in front of XL-640 become RED) to prevent accidental injury by probe.

nchsls:c:biochemistry:document:magnesium

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

**kit literature of Magnesium** New method of magnesium(online calibration) data and sample comparison data and test parameter detail Inhouse magnesium reagent preparation Reagent Management of Magnesium * Take 20 ml plastic reagent bottle.

nchsls:c:biochemistry:document:maintainence_of_zebra_tlp_2844_barcode_printer

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

parts of barcode reader to be cleaned as shown in figure [parts of barcode reader] How to clean printer head * Switch of the printer first. * Open the barcode printer by pulling two side levers towards the front of the printer. * Release the ink ribbon and pull the 'ribbon complex' upwards so that Black portion can be visible.

nchsls:c:biochemistry:document:maintainence_of_zebra_tlp_2844_barcode_printer_and_zebra_gc420t_barcode_printer

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

nchsls:c:biochemistry:document:maintainence_of_zebra_tlp_2844_barcode_printer_and_zebra_technologies_ztc_gc420t_barcode_printer

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

parts of barcode reader to be cleaned as shown in figure [parts of barcode reader] Image of printer head Image of printer sensor Image of printer roller How to clean printer head * Switch of the printer first. * Open the barcode printer by pulling two side levers towards the front of the printer.

nchsls:c:biochemistry:document:maintenance_after_filling_fluid_pack

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

* Go to maintenance: ISE Unit page.see below picture. * Click on ISE ON button. * Give purge A cycle from maintenance :ISE unit, if it give red signal repeat the cycle until it become green and if it give green signal then also repeat the purge cycle for 7 to 10 times.Repeat the same procedure with

nchsls:c:biochemistry:document:maintenance_document

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

Daily Maintenance Start of the day procedure * Empty Bio-hazardous Waste can. * Fill the Cleaning Solution can. * Clean the analyzer external surface. * Replace the printer paper if, necessary * Clean the Computer, Monitor, Keyboard And Printer External Surface.

nchsls:c:biochemistry:document:make_new_profile

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

How to make new profile * Click on erba XL-640 icon on desktop of pc. * It will ask for username and password. * Username=guest and password=???? * Click on 'Test parameter(f3)'[step 1] * Click on test you want to calibrate and then click on 'copy test'.

nchsls:c:biochemistry:document:management_of_equipment_records_in_lis

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

How to generate new equipment in database * Open 12.207.3.240/phpmyadmin and write username = root and password = ##$@. * Click on “biochemistry”. * Click on “equipment”. * Click on “insert” or “copy”. * Fill details of equipment in that empty box and click on

nchsls:c:biochemistry:document:management_of_reagent_aliquot

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

REAGENT BOTTLE MANAGEMENT PROCEDURE * Prepare 3 different container for each examination requiring small bottles for storage: * bucket for Unwashed bottle * bucket for Washed bottle * Box for in use reagent filled bottles * After making reagent, fill it in washed reagent bottle used specifically for that reagent only according to label attached (with volume dispenser defined for that reagent, where relevant) and make it air tight.

nchsls:c:biochemistry:document:maruti-weight_1kg_2kg

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

MARUTI weight 1kg identity of the equipmentweight-1kg HOSP-EQP-REG-PG-05 Sr No 140319-005manufacturer’s name, model and serial number or other unique identificationModel No:SCW-1K-F2-SS, Sr. no.140319-005contact information for the supplier or the manufacturer

nchsls:c:biochemistry:document:masterlist_of_equipment

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

Master list of Equipment * Water treatment plant * refrigerator * digital display thermometer * Digital thermometer * Centrifuge * Shimadzu Analytic Balance * Shimadzu Analytic Balance D450028778 * REPTECH Weighing Machine * Mettler Toledo Weight Machine * Sartorious loose weight * MARUTI-Weight 1kg,2kg * Digital Tachometer * Erbachem * Erba XL-640 * Printer * Barcode Printer * pH meter * ELISA reader * Micropipette * Yorco hot air oven * Erba XL-1000 * Electronic Pipe…

nchsls:c:biochemistry:document:material_resource_management_process

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

Procedure to run material resource management processProcedure for management of accommodation and environmental conditionsProcedure for management of equipmentsProcedure for management of reagents and consumablesProcedure for selecting and evaluating referral laboratories and consultants

nchsls:c:biochemistry:document:measuring_range_of_all_parameter_of_instrument

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

• When in abg report show …..? this type of sign in any parameter then advice for repeat sample • When in abg report show inreasing arrow sign or decreasing arrow sign then refer the range as shown in below image

nchsls:c:biochemistry:document:method_of_washing_cups_for_qc_and_calibrators

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

PROCEDURE FOR WASHING CUPS FOR QC AND CALIBRATORS -Wear goggles and gloves. -Take all the new cups in a rack. -Pour 10% HCL in squeezing bottle -Pour 10% HCL in each cup at a time -Wait for 10-15mins. -Wash all the cups individually with de-ionised water.

nchsls:c:biochemistry:document:mettler_toledo_weight_machine

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

Equipment(Rapidpoint-500_siemensSerial no-2741281029) Documents and RecordsRequirementType and LocationHelpa.identity of the equipmentMettler-Toledo weight machine HOSP-INSTRU-REG. PG NO-21 NO-21 Use Equipment Name and Hospital Register page Number to create ID. It is same as in LIS cl_general.equipment.equipment

nchsls:c:biochemistry:document:micro_weighing_balance

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

Equipment(Micro Weighing Balance Serial no-2741511036) Documents and RecordsRequirementType and LocationHelpa.identity of the equipmentMICRO WEIGHING BALANCE-HOSP-EQ-PG NO-6 Use Equipment Name and Hospital Register page Number to create ID. It is same as in LIS cl_general.equipment.equipment

nchsls:c:biochemistry:document:microalbumin

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

KIT LITERATURE Urinary microalbumin-Immunoturbidimetry and BCG method- parameter details, online calibration SOP,sample comparison with two method data

nchsls:c:biochemistry:document:micropipette

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

Micropipetteidentity of the equipmentREPTECH weighing Machine COLL-EQ-REG-PG-05,Sr No:1705071manufacturer’s name, model and serial number or other unique identificationREPTECH, Model No RA3102,Sr No:1705071contact information for the supplier or the manufacturer

nchsls:c:biochemistry:document:monthly_analysis_eqa_and_iqc_results

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

nchsls:c:biochemistry:document:mou_bharuch

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

nchsls:c:biochemistry:document:mou_dang

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

nchsls:c:biochemistry:document:mou_dharampur

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

nchsls:c:biochemistry:document:mou_dharampur_hospital

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

[ ]

nchsls:c:biochemistry:document:mou_navasari_mjj_hospital

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

MoU of MGG Hospital MGG Hospital Test Results Send by Them

nchsls:c:biochemistry:document:mou_navasari_vasda_koteg_hospital

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

nchsls:c:biochemistry:document:mou_of_nchs_with_smimer

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

nchsls:c:biochemistry:document:mou_valsad_gmers_medical_college

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

[ ]

nchsls:c:biochemistry:document:mou_vyara_general_hospital

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

nchsls:c:biochemistry:document:mpr

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

MPR examination procedure * Learn operation of Erba XL-640 and Erbachem. * Select equipment * Use Erba XL-640 as peferred equipment. * If Erba XL-640 is malfunctioning and can not be repaired within period acceptable to clinicians, use Erbachem and follow procedure for responding to Erba XL-640 failure *

nchsls:c:biochemistry:document:mpr_reagent

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

[MPR reagent & calibrators & QC]

nchsls:c:biochemistry:document:na

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

Sodium examination procedure * Learn operation of Erba XL-640 and Erbachem. * Select equipment * Use Erba XL-640 as peferred equipment. * If Erba XL-640 is malfunctioning and can not be repaired within period acceptable to clinicians, use Erbachem and follow procedure for responding to Erba XL-640 failure

nchsls:c:biochemistry:document:nabl_112_requirement_for_ilse

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

Alternative approaches For those tests where a formal EQA is not available, the laboratory shall adopt alternative approaches to validate performance. Such alternative approaches are: * Replicate testing * Examination of split samples (within the laboratory)

nchsls:c:biochemistry:document:network_connection_of_opd_receiving_computer

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

nchsls:c:biochemistry:document:note_on_using_electronic_pipette_for_elisa

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

For Conjugate * Set aspiration speed = 3. * Set dispense speed = 3. * While dispensing pipette, tip should be touching the upper wall of well. * Keep pressing button and move tips upward. * Only when tips are away from well, release the pressed button.do not change tip every time.

nchsls:c:biochemistry:document:nt-_pro_bnp2

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

** Instruction for NT-PRO BNP calibrator ** * 3 levels of calibrator ,in ready to use form ,liquid kept at 2 to 8 degree celcius temperature * for each level 3 allicorte of 300 microliter was made and parafilmed ,barcode label sticed and kept in urine micro container and that were kept in box and labelled as

nchsls:c:biochemistry:document:online_information_resource_for_patients_and_users

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

Clinical Biochemistry Laboratory, Near Blood Bank, New Civil Hospital, Majura Gate, Surat Phone: 0261-2208366, 0261-2208317 biochemistrygmcs@gmail.com Opening hours: 24×7 Scope http://12.207.3.240/cl_general/single_table_edit.php]]describes various examinations offered by the laboratory and relevent details. The information is available to everybody. While some details describe in scope are useful only to the laboratory personnel, others are useful to clinicians and patients.

nchsls:c:biochemistry:document:open_10.207.3.246_or_scanner_ip_settings

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

* Open 12.207.3.246 from web browser and window will apear. * Click on “Log in” in window menu. * Write “admin” as username and don't write password. After that click on “log in”. * Open a window and click on “Address book” on side of window.

nchsls:c:biochemistry:document:operator_s_guide

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

nchsls:c:biochemistry:document:osmolarity

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

CALCULATION OF SERUM OSMOLARITY following parameters required * SODIUM * GLUCOSE * UREA equation IQC is not done for osmolarity. EQAS is done every month. *

nchsls:c:biochemistry:document:page

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

[PAGE SOP] -How to perform PAGE [Page SOP excel sheet]

nchsls:c:biochemistry:document:paper-printed_report

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

How to inform Paper-printed report * Open 12.207.3.240 * Enter your user name and password. * Go to 'Report--->Print-PDF-A4' .[Step 4] * Enter sample Id in from_sample_id and to_sample_id.select Authorised signatory. * Click on 'Print' * Report will open in pdf format and print in Ricoh printer.

nchsls:c:biochemistry:document:pepsin_wash

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

|PROCEDURE FOR MAKING PEPSIN FOR ISE WASH ( current method )}} [PROCEDURE FOR MAKING PEPSIN FOR ISE WASH ( old method )] preparation of pepsin rack picture of how to filter reagent Checking Pepsin activity * Take fibrin clot ( from PT tube/ clotted sample) . * Take 2 small fibrin clots in 2 different glass tubes.which is shown below .clot in pepsin turn into dark red colour due to formation of acid hematin (reaction between hemoglobin with HCL present in pepsin)

nchsls:c:biochemistry:document:persons_authorised_to_operate_erba-xl_640

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

Persons authorized to operate ERBA-XL 640 in addition to authorised signatories Dr.NeelamDr.DeeptiDr.ManmeetDr.RiddhiDr.KhushbuMr.ManharMr.ShankarMr.VireshMr.DharmeshMr.MaheshMr.KishorMr.RameshMrs.TruptiMiss.ManishaMrs.NiharikaMiss.RoshniMiss.KomalMiss.Purva

nchsls:c:biochemistry:document:persons_authorised_to_operate_erbachem

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

Persons authorized to operate erbachem in addition to authorised signatories Dr.NeelamDr.DeeptiDr.ManmeetDr.RiddhiDr.KhushbuMr.ManharMr.ShankarMr.VireshMr.DharmeshMr.MaheshMr.KishorMr.RameshMrs.TruptiMiss.ManishaMrs.NiharikaMiss.RoshniMiss.KomalMiss.Purva

nchsls:c:biochemistry:document:ph_calibrators

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

nchsls:c:biochemistry:document:ph_meter

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

* Calibration measurement and maintenance of pH meter * User manual General Information pH Measurement with a Glass Electrode * Glass electrode is most common ion-selective electrode * Combination electrode incorporates both glass and reference electrode in one body .

nchsls:c:biochemistry:document:phenol_for_orthopedic_purpose

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

Phenol used for ADA purpose is 7-10% in concentration. For orthopedics purpose, they need 5% phenol. How to make 5% phenol?? so we take 5 phenol cups (containing 1 ml in each cup and its concentration is 7-10%) in sterile micro cup and then add 5 ml of DI water into it.

nchsls:c:biochemistry:document:phosphorus

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

Procedure for Phosphorus examination * Learn operation of Erba XL-640 . * Select equipment * Use Erba XL-640 as preferred equipment. * If Erba XL-640 is malfunctioning, stop the examination and follow procedure for responding to Erba XL-640 failure * Read following documents referencesPhosphorus Reference Reagent}}

nchsls:c:biochemistry:document:postexamination_process

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

Procedures to run Postexamination processProcedure for review of resultsProcedure for storage, retention and disposal of clinical samplesProcedure for reporting of resultsProcedure for release of results

nchsls:c:biochemistry:document:preexamination_process

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

procedures to run Preexamination processProcedure for informing patients and users about the laboratory servicesProcedure for sample collection and transportationProcedure for receiving sampleProcedure for pre-examination handling, preparation and storage

nchsls:c:biochemistry:document:preparation_before_doing_procedure_for_elisa

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

* put incubator at 24'C at least 1 hour before starting procedure. * put all standards and enzyme conjugate reagent in incubator at 24'C for 30 minutes. * put electronic multichannel pipettes in charging. * Prepare worklist of samples in which TSH or Free T4 is to be estimated which includes the plan that in which well which sample is to be put.Standard ,control, QC and Samples are run in duplicate.They are placed in mirror image form

nchsls:c:biochemistry:document:preparation_of_iqc

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

nchsls:c:biochemistry:document:prepare_randox_cal_2_and_cal_3

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

* Take 5 ml Qc water(DI water of 0.00 microsiemens/cm) in volumetric flask. * Gently transfer water in cal 2 and cal 3 bottle.Pour last drop of water by simply rotate flask over edge of bottle.Do not beat the flask over bottle. * Mix it by gentle inversion and eversion.

nchsls:c:biochemistry:document:preparing_aliquot

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

Preparation of Aliquots * Write sample id on ependroff cups with permanent marker pen on the rough surface of ependroff cup. * Arrange them in ascending order in the rack. * Aspirate serum from the centrifuged vacutte with the help of 200 uL pipette.

nchsls:c:biochemistry:document:preparing_che_epindroff_cups

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

Preparation of CHE epindroff cups * Put electronic pipette in charging 1 hour before starting procedure. * CHE kit provide R1 and R2. * Dissolve 30 ml DI water in R1 bottle and 1 ml DI water in R2 bottle. * Kept it for 15 min. Meanwhile keep ependroff cup label with 'CHE' and parafilm of appropriate size ready.

nchsls:c:biochemistry:document:preparing_che_reagent

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

preparation of cholinesterase reagent * dissolve R1 in 30 ml and R2 in 1 ml of DI water * mix R1 and R2 after 15 min after complete dissolving of R1 and R2 * aliquot 1 ml of reagent in plain vaccutte after proper washing of vaccutte * keep frozen

nchsls:c:biochemistry:document:print_barcode

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

[User manual of Zebra tlp2844 whole][User manual of Zebra tlp2844 Figures][User manual of Zebra tlp2844 programming guide][User manual of Zebra_Technologies_ZTC_GC420t_ Barcode printer]Installation and troubleshooting of Zebra tlp 2844 Barcode printerMaintainence of Zebra tlp 2844 Barcode printer and Zebra_Technologies_ZTC_GC420t_Barcode printerInstallation and troubleshooting of Zebra_Technologies_ZTC_GC420t_ Barcode printerAutocalibration and manual calibration of Zebra tlp 2844 Barcode printe…

nchsls:c:biochemistry:document:print_examination_wise_worklist

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

How to print examination wise worklist * Open 12.207.3.240. * Enter user name and password.[step 1] * Click on worklist--->'Examination wise worklist' * Enter 'from_sample_id' to 'to_sample_id' as shown in screenshot. * Click on name of test which you want to print worklist.

nchsls:c:biochemistry:document:print_report

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

Print report * Press 'ctrl+p' and select printer 'MP_2001L' as shown in screenshot.[step1] * Click on 'Print'. * Installation and troubleshooting of Ricoh MP_2001L * Installation and troubleshooting of Ricoh aficio MP 2000

nchsls:c:biochemistry:document:printed_report

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

How to inform Paper-printed report * Open 12.207.3.240 * Enter your user name and password. * Go to View. * Click on By Sample ID. * Following screen will be seen. * * Enter Sample ID which you want to release. * Following screen will be seen.

nchsls:c:biochemistry:document:printer

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

Ricoh Printer MP 2000 L2 identity of the equipmentRicoh Printer MP 2000 L2 COLL EQ P-123-17126750553manufacturer’s name, model and serial number or other unique identificationRicoh MP 2000 L217126750553contact information for the supplier or the manufacturer

nchsls:c:biochemistry:document:procalcitonin

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

Special instruction for hs Tn-I calibrator * 2 level of calibrator level-1 , level-2 * It comes in frozen state and ready to use form * Each level contain 2 ml of calibrator * From main bottle 4 cup was made of 0.5 ml from each level * This cup was capped and parafilm done

nchsls:c:biochemistry:document:procedure_after_erba_xl-640_probe_injury

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

Procedure after Erba XL-640 probe injury First Aid- At Site of Exposure * Wash exposed part with water or Normal Saline * Do not Squeeze the Wound to bleed it * Do not use any Disinfectant over exposed site Dose of post exposure prophylaxis

nchsls:c:biochemistry:document:procedure_for_action_during_electrical_failure

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

Procedure for action during electrical failure * Call for electrician at 701 extension number. * Because the call may not be answered or may not be taken seriously, send a personnel to the electrical department. * If necessary,shutdown some of the less essential equipment like computer and centrifuge may be switched off.

nchsls:c:biochemistry:document:procedure_for_erba_xl-640_software_installation

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

Procedure for ERBA XL-640 Software Installation Two files required for Erba MultiXL software as follows * [Database Utility] * [SetUp] In setup.zip file for Erba MultiXL software * there will be two files. * 1)MultiXl.msi * 2)setup.exe * Run “setup.exe”

nchsls:c:biochemistry:document:procedure_for_erba_xl-640_wash_solution_preparation

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

Procedure for Erba XL-640 wash solution preparation * Take 1 ml of extran solution in 100 ml bottle specific for Wash solution preparation. * Fill rest of bottle with DI water.

nchsls:c:biochemistry:document:procedure_for_instrument_acceptibility

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

**Procedure For Instrument Acceptibiliy** == Set Up The Lab For Instrument == * Space for Instrument * Water Supply * Electricity Supply * Waste Maagement == Installation Of Instrument == * Get install Instrument by Its company person(Engineer).

nchsls:c:biochemistry:document:procedure_for_lis_verification

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

Procedure For LIS Verification When to do LIS verification LIS verification should be done after installation, after every change done in LIS and periodically 6 monthly. The general process should involve i.Take 10 samples, do entry in LIS and analyze in instrument.

nchsls:c:biochemistry:document:procedure_for_maintenance

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

Page number of User manual is given as reference. * 11.1 Daily Maintenance- 507 * 11.2 Weekly Maintenance- 508 * 11.3 Quarterly Maintenance- 509 * 11.4 Annual Maintenance- 510 * 11.6 Preventative Maintenance- 512

nchsls:c:biochemistry:document:procedure_for_operating_erba_xl-640

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

1. Starting Power * Switch on main(wall) supply * Start UPS supply by pressing ON-button for few seconds * Switch on Erba XL-640 back-power-supply (Wait for 2 min) * Do not Switch on Erba XL-640 side-power-supply * (side-power-supply=OFF > Front light=RED)

nchsls:c:biochemistry:document:procedure_for_performance_qualification

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

Design Qualification Document * A document showing what to do AFTER equipment is produced and BEFORE it is put into actual use * It will include (=defines) * Procedure for installation Qualification * What is required for installation? *

nchsls:c:biochemistry:document:procedure_for_performance_qualification_hplc

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

nchsls:c:biochemistry:document:procedure_for_responding_to_erba_xl-640_failure

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

Procedure for responding to Erba XL-640 failure * Call Jiteshbhai(9327110049) for early Repair of Auto analyser. * If required follow following troubleshooting * Prepare All 3 Semiauto analyser and Prolyte for 5 Test that is Creatinine , Bilirubin , Glucose, ALT and Electrolyte.

nchsls:c:biochemistry:document:procedure_of_changing_the_lamp

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

nchsls:c:biochemistry:document:procedure_of_ise_cleaning_solution

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

[PROCEDURE FOR MAKING PEPSIN FOR ISE WASH ( current method )] [PROCEDURE FOR MAKING PEPSIN FOR ISE WASH ( old method )] Checking Pepsin activity * Take fibrin clot ( from PT tube/ clotted sample) . * Take 2 small fibrin clots in 2 different glass tubes. * Add 0.5 ml di water in 1 glass tube. This is control. * Add 0.5 ml pepsin in another glass tube . This is test sample.

nchsls:c:biochemistry:document:prolactin

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

** Instruction for prolactin calibrator ** * 2 level of calibrator, in frozen powered form * keep at room temperature and than reconstitute with 1 ml of QC water * Make 5 cup of 200 microliter of both level * Label those cup and parafilm it

nchsls:c:biochemistry:document:prolyte

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

nchsls:c:biochemistry:document:qa_forum_sample

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

How to make cup for QAF SAMPLE How to process QAF SAMPLE How to send QAF Results How to make QAF six monthly file as Record

nchsls:c:biochemistry:document:quality_control_process

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

Biochemistry-specific procedure to run quality control processProcedure for ensuring quality of examination resultsGeneral procedures to run quality control processDocument Control ProcedureProcedure for identification, control, correction and prevention of nonconformitiesProcedure for control of recordsProcedure for evaluation, audits and resolution of complaints|Procedure for management reviewProcedure for monitoring quality indicators

nchsls:c:biochemistry:document:rapidpoint-500_siemens

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

Equipment(RAPIDPOINT 500e SIEMENS_SR NO-53726_MODEL NO-11416755_HOSP-EQ-PG-80) Documents and RecordsRequirementType and LocationHelpa.identity of the equipmentRAPID POINT 500e SIEMENS_SR NO-53726_MODEL NO-11416755_HOSP-EQ-PG-80 Use Equipment Name and Hospital Register page Number to create ID. It is same as in LIS cl_general.equipment.equipment

nchsls:c:biochemistry:document:raw_data_for_eqa_for_vitros_3600_chemianalyzer_tests

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

RAW DATA FILE FORMAT * This is how all raw data is being stored for VITROS 3600 EQC Sample. * This is the file format. * MONTHLY DATA FILE FORMAT * This is how monthly file is being stored for VITROS 3600 EQC Sample. * This is the file format.

nchsls:c:biochemistry:document:reagent_bottle_preparation_and_programming_of_it_in_erba_xl-640

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

Reagent bottle preparation and programming of it in Erba XL-640 * Wash reagent bottle 2-3 times with DI water. * Then fill the reagent of test you want to calibrate. * Give position of new reagent bottle in erba XL-640. * Go to 'Utility ---> Reagent position(f11)'.

nchsls:c:biochemistry:document:refrigerator

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

General Instruction * Keep a calibrated digital display thermometer in the refrigerator/deep freeze. * Read temperature of refrigerator in morning record in [LIS] * NOTE: when u note down temperature of refrigerator, make sure that refrigerator door is not opened since half an hour otherwise reading will be higher than actual temperature.

nchsls:c:biochemistry:document:release_of_reports

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

HOW TO RELEASE A REPORT * Go the url . * then login with the password. * Follwing screen will be shown * * click on view--->sample id. * Following screen will be shown. * * insert sample ID which we want to release * For example

nchsls:c:biochemistry:document:reorder_point

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

* The reorder point (ROP) is the level of inventory which triggers an action to replenish that particular inventory stock. It is a minimum amount of an item which a firm holds in stock, such that, when stock falls to this amount, the item must be reordered. It is normally calculated as the forecast usage during the replenishment

nchsls:c:biochemistry:document:report

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

nchsls:c:biochemistry:document:reports

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

* Go the url . * then login with the password. * Follwing screen will be shown * * click on view--->sample id. * Following screen will be shown. * * insert sample ID which we want to release * For example “1100212”, * *

nchsls:c:biochemistry:document:reptech_weighing_machine

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

REPTECH weighing Machine Sr.No:1705071identity of the equipmentREPTECH weighing Machine COLL-EQ-REG-PG-05,Sr No:1705071manufacturer’s name, model and serial number or other unique identificationREPTECH, Model No RA3102,Sr No:1705071contact information for the supplier or the manufacturer

nchsls:c:biochemistry:document:revised_reports

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

Procedure for withdrawn report * When you find wrong value in report. * Click on “Edit” * You will find this window shown below. * * Click on Examinations. * Click on BI. * You will find this window shown below. * * Scroll it to the end.

nchsls:c:biochemistry:document:riqas

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

* Monitor * corrective action * defined responsibilities * instructions for participation * performance criteria that differ from the criteria used in the interlaboratory comparison programme

nchsls:c:biochemistry:document:run_samples_in_erba_xl-640

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

How to run samples on Erba XL-640 * Click on erba XL-640 icon on desktop of pc. * It will ask for username and password. * Username=tech and password=???? * Click on 'patient entry' in Erba XL-640 software.[step 1] * Simutaneously open 12.207.3.240 in mozilla firefox.Pin up the mozilla firefox window with deskpin

nchsls:c:biochemistry:document:sample_preparation_for_hdl

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

nchsls:c:biochemistry:document:sample_preparation_for_tibc

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

Sample preparation for TIBC * Print examination wise worklist for TIBC. * Write sample Id on rough part of ependroff cup according to worklist. * Dispense 400 microliter iron standard(500 microgram/dl) in each ependroff cup. * Add 200 microliter sample in ependroff cup after matching sample ID written on ependroff with vacutte.

nchsls:c:biochemistry:document:sample_preservation_for_free_t4

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

Sample preservation for free T4 * After TSH examination completed on tuesday and friday , Look for sample having TSH < 0.5 and TSH > 5.0. * All these samples having TSH < 0.5 and TSH > 5.0 preserved in deep freeze.

nchsls:c:biochemistry:document:sample_preservation_for_tsh

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

Sample preservation of TSH * Print examination wise worklist for TSH twice a day at 9 AM and 4 PM. * Write sample Id on rough part of capped ependroff cup according to worklist. * Take minimum 200 microliter serum and maximum as much as possible in capped epindroff cup. *

nchsls:c:biochemistry:document:sample_processing_in_xl-1000

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

Sample Processing in Erba XL-1000 * Check Communication * For power supply * check power cable (grey colour) properly fit or not in machine. * For COMPORT * Check comport cable (blue) properly fit or not in machine. *

nchsls:c:biochemistry:document:sample_receipt_tosoh-_hplc

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

print 2 barcode sticker * * from 1st barcode sticker,small cut part affix on cap of vacuette and remaining part on Ward Log-Book. * * * [__Underlined Text__]

nchsls:c:biochemistry:document:sample_receipt_xl-1000

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

Sample Receipt XL-1000 * print 2 barcode sticker * * from 1st barcode sticker,small cut part affix on cap of vacuette and remaining part on Ward Log-Book. * * * [__Underlined Text__] * 2nd barcode sticker affix on vacuette vertically. * barcode apply on white sticker of vacuette.

nchsls:c:biochemistry:document:sample_retention_after_processing_in_xl-1000

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

After analysis of barcoded sample in erba XL 1000 is completed, 1.sample is put back in thermocol rack according to its ID. 2.Thermocol rach put in centifuge area.

nchsls:c:biochemistry:document:sample_retention_in_xl-1000

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

After analysis of barcoded sample in erba XL 1000 is completed, 1.sample is put back in thermocol rack according to its ID. 2.Thermocol rach put in centifuge area.

nchsls:c:biochemistry:document:sartorious_loose_weight

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

Sartorious loose weight(100mg) identity of the equipmentLoose weight-100mg HOSP-EQP-REG-PG-13 manufacturer’s name, model and serial number or other unique identificationSartorious-YC-W2-13-00, Sr. no.24929447contact information for the supplier or the manufacturer

nchsls:c:biochemistry:document:schedule_for_performing_tsh_and_free_t4_examination

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

Name of ExaminationDays of weekTSHTuesday and FridayFree T4SaturdayResults of samples requiring dilutionNext batchReporting of TSH and Free T4Every Monday

nchsls:c:biochemistry:document:schedule_for_preventive_maintenance

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

[Schedule for Preventive maintainance] * Change 2nd sediment filter with new one every 15 days , and replace the first filter with the second one.Discard the first filter. (First filter in the way of inlet tube.Second filter after carbon block and towards the RO membrane.) * TROUBLESHOOTING WATER TREATMENT PLANT

nchsls:c:biochemistry:document:separating_buffer_ph_8.8_4x

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

preparaion of separation buffer pH8.8,4X * tris base= 18.171gm * Conc. HCl= 2.0081ml * Check pH and adjust to 8.8 with HCL if required

nchsls:c:biochemistry:document:serum_protein_electrophoresis

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

Serum protein Electrophoresis examination procedure * Learn operation of Electrophoresis tank and Electrophoreis Power supply. * Read following documents References [SPE Reference literature]environmental and safety controls [MSDS of agarose] [MSDS of Amido black] [MSDS of Tris base] [MSDS of methanol] [MSDS of acetic acid] Introduction Electrophoresis is a comprehensive term that refers to the migration of charged solutes or particles of any size in a liquid medium under influence of an …

nchsls:c:biochemistry:document:shimadzu_analytic_balance

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

shimatzue electronic weight balance AUW220Didentity of the equipmentshimatzue electronic weight balance HOS-EQ-REG-PG-21manufacturer’s name, model and serial number or other unique identificationshimatzue electronic weight balance AUW220Dcontact information for the supplier or the manufacturer

nchsls:c:biochemistry:document:shimadzu_analytic_balance_d450028778

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

shimatzue electronic weight balance D450028778identity of the equipmentshimatzue electronic weight balance HOS-EQ-REG-PG-21manufacturer’s name, model and serial number or other unique identificationshimatzue electronic weight balance AUW220Dcontact information for the supplier or the manufacturer

nchsls:c:biochemistry:document:sodium_azide

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

preperation of sodium azide reagent.

nchsls:c:biochemistry:document:sop_for_ilse

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

nchsls:c:biochemistry:document:sop_of_barcode_setting

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

Steps FOR BARCODE ASETTING * this setting is done by engineer and for information purpose only * Frist go to menu and then utility,then bcr * * * * * * * * *

nchsls:c:biochemistry:document:sop_of_il-6

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

1)GO TO MANU TEST AS SHOWN IN BELOW IMAGE 2)ENTER PATIENT SAMPLE ID AS SHOWN IN BELOW IMAGE 3)THEN SCAN BARCODE OF TEST STRIP AS SHOWN IN BELOW IMAGE [{{:nchsls:c:biochemistry:document:image_-3.jpg|]}} 4)INSERT THE TEST CARD AS SHOWN IN BELOW IMAGE 5)THEN PRESS ENTER BUTTON TO TEST 7)PATIENT RESULT WILL APPEAR AS SHOWN IN BELOW IMAGE

nchsls:c:biochemistry:document:sort_data_for_new_qc_in_excel_sheet

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

Sort data for new QC in Excel sheet * Overlap New Qc samples with old Qc samples for 20 days. * After end of Batch , Export results from Erba XL-640. * Open 10.207.3.245 from any computer.Following window opens.[step 1] * Open last file of “result reprint”. It open like this.

nchsls:c:biochemistry:document:special_instruction

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

special instruction for vitros-3600 * Do not forget to do microwell incubator reset after claning of microwell incubator , For microwell incubator reset go to Diagnostics-MEDS-INCUBATOR AND TRANSPORTS and click on Reset uW Inc * To prevent two test waste go to option and configuration menu then configure system then sample/result option keep

nchsls:c:biochemistry:document:special_instruction_for_hs_troponin-i_calibrator

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

nchsls:c:biochemistry:document:special_instructions_for_covid19

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

Special Instructions for COVID19 Samples (For Lab Technicians) * This instructions are relevant for non-covid19 samples,too * There are two types of MRD numbers for COVID19 samples * Example1: 05/123 (Where 05 is month) * Example2: SUR/20/00123456 (8 digits mandatory)

nchsls:c:biochemistry:document:stacking_buffer_ph_6.8_4x

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

Preparation of Stacking buffer, pH 6.8, 4X * Tris Base= 6.057gm * Conc. HCl= 4.1213ml * Make up to 100ml * Check pH.

nchsls:c:biochemistry:document:start_pushing_up_of_scanner

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

Following steps taken for establishing connection between scanner and computer: * * Give permission to allow scanning. * Install vsftpd software from synaptic package manager. * Start sevice of vsftpd * Scan dummy file to conform whether connection is established or not.

nchsls:c:biochemistry:document:start_sevice_of_vsftpd

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

To check file location in system * Write below command. For start working of vsftpd software * Write command as below in terminal. * Make following two changes in vsftpd.conf file by uncommenting two lines. * Press Ctrl + O and enter.

nchsls:c:biochemistry:document:stop_reflux_testing_from_server

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

How to stop reflux testing from server * Open terminal from any computer which is connected to server. * Type following commands as shown in screenshot. * As we want to access server computer from another computer type command 'ssh root@12.207.3.240'

nchsls:c:biochemistry:document:sudden_fall_in_lamp_gain

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

When there is sudden fall in lamp gain (below 60) and erba shows error that “change in lamp gain” -Check all tubings of laundry system. -If there is any minor blockage in any of its tubings, there will be no flow of water so all cell blanks will be zero. and all qc fall on lower side or negative results will come.

nchsls:c:biochemistry:document:tbil

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

Total bilirubin examination procedure * Learn operation of Erba XL-640 and Erbachem. * Select equipment * Use Erba XL-640 as peferred equipment. * If Erba XL-640 is malfunctioning and can not be repaired within period acceptable to clinicians, use Erbachem and follow

nchsls:c:biochemistry:document:teb_buffer

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

ChemicalquantityTris base10.2 gmEDTA powder0.6 gmBoric acid3.2 gm Make up to 1000 ml. Make sure that its pH is 8.6

nchsls:c:biochemistry:document:teb_buffer_ph_8.6

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

ChemicalquantityTris base10.2 gmEDTA powder0.6 gmBoric acid3.2 gm Make up to 1000 ml. Make sure that its pH is 8.6

nchsls:c:biochemistry:document:telephonically_released_report

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

How to inform Report Telephonically * When there is any inquiry about results of reports from ward, we are bound to receive call and answer them accordingly. * Technicians inform results on call and again ask them to cross verify the results and ensure that opposite person on phone heard exact values which is being informed to them.

nchsls:c:biochemistry:document:test_parameter_for_che_in_erba_xl-640

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

set following parameter

nchsls:c:biochemistry:document:test_parameter_settings

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

There is confusion in user manual about meaning of reaction absorbance limit. Experiments showing results:}} Instructions * two point (increasing) * example creatinine * difference between M1 and M2 is used. (delta OD) * SO, perform lineality experiment, find upper limit (18 mg approx)

nchsls:c:biochemistry:document:tg

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

Triglyceride examination procedure * Learn operation of Erba XL-640 and Erbachem. * Select equipment * Use Erba XL-640 as peferred equipment. * If Erba XL-640 is malfunctioning and can not be repaired within period acceptable to clinicians, use Erbachem and follow

nchsls:c:biochemistry:document:tibc

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

TIBC examination procedure * Learn operation of Erba XL-640 and Erbachem. * Select equipment * Use Erba XL-640 as peferred equipment. * If Erba XL-640 is malfunctioning and can not be repaired within period acceptable to clinicians, use Erbachem and follow procedure for responding to Erba XL-640 failure *

nchsls:c:biochemistry:document:tibc_reagent

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

nchsls:c:biochemistry:document:tosoh_automated_glycohemoglobin_analyzer_hlc-_723_gx

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

1^Equipment(TOSOH automated glycohemoglobin analyzer HLC- 723 GX) Documents and Records^^^ RequirementType and LocationHelpa.identity of the equipmentTOSOH automated glycohemoglobin analyzer_SR NO-1182094_MODEL NO-HLC- 723 GX_HOSP-EQ-PG-04 Use Equipment Name and Hospital Register page Number to create ID. It is same as in LIS cl_general.equipment.equipment

nchsls:c:biochemistry:document:total_beta-hcg_2

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

Instruction for Beta -hCG calibrator * 3 level of calibrator,frozen powdered form * keep at room temperature for 10-15 min * reconstitute with 1 ml of QC water * Make 5 cup of 200 microliter of each level * 1 cup of each level will be used for calibration

nchsls:c:biochemistry:document:total_bilirubin_reagent

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

[Total Bilirubin Reagent]

nchsls:c:biochemistry:document:total_protein

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

Total protein examination procedure * Learn operation of Erba XL-640 and Erbachem. * Select equipment * Use Erba XL-640 as peferred equipment. * If Erba XL-640 is malfunctioning and can not be repaired within period acceptable to clinicians, use Erbachem and follow

nchsls:c:biochemistry:document:total_protein_reagent

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

Making Reagent [Protein_reagent]

nchsls:c:biochemistry:document:training_points

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

Training Points * Wrong placement of tube on sample-rack, photo * Method of changing lable alignment in rack * Keeeping rack on trackt * manage TRAIN * How to attach label on cap * primary and secondary power switch of instrument * soft shutdown, hard shut down

nchsls:c:biochemistry:document:training_points_for_abg

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

nchsls:c:biochemistry:document:training_points_for_vitros-3600

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

---------- ---------- DAILY MAINTENANCE * 1.Go to diagnostic mode * 2.Then periodic maintenance * 3.Do daily maintenance * 4.Use 70% isopropyl alcohol,DI water and one unused handkerchief from daily maintenance box * 5.Perform all nine step and save it at the end.

nchsls:c:biochemistry:document:trial

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

[ADA format]

nchsls:c:biochemistry:document:trichloro_acetic_acid

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

10% TCA(TRI CHIORO ACETIC ACID) * Take 10 gm of 99% TCA in microcup approx.and immediately cap the bottle as it is in crystal form and very hygroscopic and weigh it on shimadzu machine for 10 gm. * Take approximately 50 ml of DI water in volumetric flask and add 10 gm of 99% TCA powder.

nchsls:c:biochemistry:document:tropi_pct_probnp_mean_and_sd_data

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

nchsls:c:biochemistry:document:trouble_shooting

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

TROUBLESHOOTING: 1. Instruction for reagent tray adaptor management 2. Continuous Dirty Cuvette messages

nchsls:c:biochemistry:document:trouble_shooting_for_internet_conection_error_in_lab

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

Internet faliure in lab * When there is complain from resident for lis report site not open then chek point as written below * First check internet in all comuter then chek gmcsurat.edu.in site is open from computer or not and also from your phone

nchsls:c:biochemistry:document:trouble_shooting_opd_computer

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

OPD server failure * open terminal * write - ssh.root@12.207.3.240. * give password:- * -it will show the screen as follows - -Enter ping 10.206.10.247 -it will show the screen below if working - -it will show the screen below if not working -

nchsls:c:biochemistry:document:trouble_shootinh_of_ricoh_2702

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

When printer show following message

nchsls:c:biochemistry:document:troubleshooting

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

**Instruction for host code** In patient sample machine has taken sample for hsTrop-I 3 times but did not taken for NT-proBNP-2 and it was due to host code of hsTrop-I and NT-proBNP-2 was same To prevent such type of mistake in future enter host code of new examination by seeing it in host code pdf

nchsls:c:biochemistry:document:troubleshooting_of_abg_instrument

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

nchsls:c:biochemistry:document:troubleshooting_of_opd_report_dispatch_computer

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

**What to do when Biochemistry LIS will not open at OPD report dispatch computer** * First go to Control panel from start menu * * Than go to network and internet * * Than go to network and sharing center * * Than go to change adaptor settings

nchsls:c:biochemistry:document:tsh

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

Procedure for TSH examination referencesenvironmental and safety controls[MSDS of TSH kit ]CentrifugationSample preservation for TSH How to perform TSH examination Schedule for performing TSH and Free T4 examination ** Instruction for TSH calibrator ** * 3 levels of calibrator ,in ready to use form ,liquid kept at 2 to 8 degree celcius temperature * for each level 7 allicorte of 300 microliter was made and parafilmed ,barcode label sticed and kept in urine micro container and that …

nchsls:c:biochemistry:document:tsh_examination

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

Preparation before doing procedure for ELISA Procedure for TSH examination * Dispense 200uL samples in wells of dummy plate according to worklist using 200 uL pipette. * Take out the microtiter plate. Don't touch the lower surface of the plate. * Dispense 50 uL sample from the dummy plate into the microtiter plate using multichannel pipette.Note the time of starting dispensing of sample.

nchsls:c:biochemistry:document:ua

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

Uric acid examination procedure * Learn operation of Erba XL-640 and Erbachem. * Select equipment * Use Erba XL-640 as peferred equipment. * If Erba XL-640 is malfunctioning and can not be repaired within period acceptable to clinicians, use Erbachem and follow

nchsls:c:biochemistry:document:uibc

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

UIBC examination procedure * Learn operation of Erba XL-640 and Erbachem. * Select equipment * Use Erba XL-640 as peferred equipment. * If Erba XL-640 is malfunctioning and can not be repaired within period acceptable to clinicians, use Erbachem and follow procedure for responding to Erba XL-640 failure *

nchsls:c:biochemistry:document:upload_communications

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

Upload communication * Open 12.207.3.240 * Enter your user name and password.[figure 1] * Click on Documents/Records ----> Upload in main menu. * Select file whichever wanted to upload in browse. * Select location where file have to be uploaded. *

nchsls:c:biochemistry:document:upt_immunoassay_analyzer_for_il-6

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

Equipment(UPT Immunoassay Analyzer_SR NO-SU3A20090554SEN_MODEL NO-UPT-3A-1800_HOSP-EQ-PG-25) Documents and Records^^^ RequirementType and LocationHelpa.identity of the equipmentUPT Immunoassay Analyzer_SR NO-SU3A20090554SEN_MODEL NO-UPT-3A-1800_HOSP-EQ-PG-25

nchsls:c:biochemistry:document:urea

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

Procedure for Urea examination * Learn operation of Erba XL-640 and Erbachem. * Select equipment * Use Erba XL-640 as preferred equipment. * If Erba XL-640 is malfunctioning, stop the examination and follow procedure for responding to Erba XL-640 failure * Read following documents * urea erba kit literaure

nchsls:c:biochemistry:document:urine_diluent_mops

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

URINE MOPS DILUENT AND SAMPLE PREPARATION URINE MOPS DILUENT REFERENCE LITERATURE- How to prepare Urine QC??? \ Url link for recovery experiment in clinical chemistry:-

nchsls:c:biochemistry:document:urine_osmolality

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

Procedure for Urine Osmolality * Run as urine * Give Na,K,Creatinine,Glucose,Urea tests in urine * Urine osmolality will be calculated * Urine Osmolality=2xNa + 2xK+UREA/2.14/2.8+GLUCOSE/18 * 2.8-For mg to mmol * 18 for mg to mmol *

nchsls:c:biochemistry:document:using_departmental_internet_connection

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

Using departmental internet connection * Open preferences. * * open ADVANCED window, then go to settings * * following settings should be done in connection settings * * Press OK to save change.

nchsls:c:biochemistry:document:vit_b12

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

B12 Reagent Explanation * One uncoated cup from Reagent 2 is taken * Serum added in it * Alkanine reagent Reagent 3-1 is added * Serum B12-Tc complex dissociate at alkaline pH and Tc get denatured (Reagent 3-1) * Reagent 3-2 (neutralizing reagent) added

nchsls:c:biochemistry:document:vitros-3600_lis_connection_image

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

nchsls:c:biochemistry:document:vitros_3600_eqc_sample

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

EQA For VITROS_3600 Chemianalyzer TestsRAW DATA FOR EQA For VITROS_3600 Chemianalyzer Tests

nchsls:c:biochemistry:document:vitros_cheminalyzer

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

Equipment Documents and RecordsRequirementType and LocationHelpa.identity of the equipmentVITROS 3600-HOSP_EQ-P-Use Equipment Name and Hospital Register page Number to create ID. It is same as in LIS cl_general.equipment.equipment upload pdf of orders

nchsls:c:biochemistry:document:vldl_cholesterol

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

VLDL cholesterol examination procedure * Read following documents references[VLDL reference literature] Calculation Of LDL / VLDL by manually * Formula for LDL calculation = Total cholesterol - (HDL + VLDL) * VLDL = TG/5 , VLDL can be calculated by this freidwald formula if TG ≤ 400 mg/dL

nchsls:c:biochemistry:document:washing_of_cups_for_testing_of_patient_samples

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

PROCEDURE FOR WASHING OF CUPS FOR REUSAGE (for patient' samples) -Wear goggles and gloves. -Take all the cups in a bucket containing 1% Sodium Hypochlorite -Wash the cups under running tap water. -Now wash the cups with de-ionised water for 4 consecutive times.

nchsls:c:biochemistry:document:water_saturated_with_butanol

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

Preparation of water saturated with Butanol * Butanol saturated with water can harm acrilic of equipment * Add 200ml butanol in 1 lit water. Mix. Allow phase separation * Take infranatent using separating funnel * Add small amount of BPB dye to help visualization of Butanol layer.

nchsls:c:biochemistry:document:water_treatment_plant

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

Water Treatment Plant (Research Lab) Water Treatment Plant (Clinical Lab) BIG Water Treatment Plant (Clinical Lab)

nchsls:c:biochemistry:document:water_treatment_plant_clinical_lab

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

identity of the equipmentDocumentmanufacturer’s name, model and serial number or other unique identificationDocumentcontact information for the supplier or the manufacturerDocumentdate of receiving and date of entering into serviceDocumentlocationDocument

nchsls:c:biochemistry:document:water_treatment_plant_general_instructions

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

HOW TO OPERATE WATER TREATMENT PLANT PARTS: * 2 Sediment filters * Carbon block * Ro membrane * Resin * Post carbon filter * U V filter * Inlet outlet tube Procedure to Operate Water Treatment Plant: * Switch on the main power supply of water treatment plant and TDS meter. Open the cork of water source

nchsls:c:biochemistry:document:water_treatment_plant_research_lab

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

identity of the equipment manufacturer’s name, model and serial number or other unique identificationHiTech, Suratcontact information for the supplier or the manufacturer date of receiving and date of entering into service locationResearch Roomcondition when received (e.g. new, used or reconditioned)

nchsls:c:biochemistry:document:wdi_for_abg

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

How to remove air from sample before analysis

nchsls:c:biochemistry:document:weely_maintenence

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

Do not forget to do microwell incubator reset after claning of microwell incubator * For microwell incubator reset go to Diagnostics-MEDS-INCUBATOR AND TRANSPORTS and click on Reset uW Inc

nchsls:c:biochemistry:document:what_to_do_when_barcode_is_not_printing_while_pressing_on_b1_icon

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

What to do When Barcode is not printing while pressing on B1 Icon?? Click on Open Menu as shown below. click on preference. In preference-->general-->Downloads-->save files-->Download Again click on open menu-->Preference-->Application. In application

nchsls:c:biochemistry:document:when_printer_show_following_message

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

1-Go to the main screen of printer as shown in image 2-Go to the second page of main screen and click on setting as shown in below image 3-On clicking on setting following screen will appear 4-click on screen feature setting and following screen will appear

nchsls:c:biochemistry:document:withdrawal_of_report

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00

nchsls:c:biochemistry:document:yorco_hot_air_oven

Anonymous (anonymous@undisclosed.example.com) — 2022-08-13T07:34:35+00:00