Dr Piyush Tailor

1. PURPOSE OF EXAMINATION: To lay down the procedure for Specimen Fixation in Histo-pathology section at NCHLS Surat

2. PRINCIPLE AND METHOD OF PROCEDURE USED FOR EXAMINATION: Formalin preserves the tissue in life like state. Approximately 16-24 hrs requires for adequate fixation. The penetration capacity of formalin is 1 cm so large specimens shall be cut into multiple slices of 1 cm thickness.

3. PERFORMANCE CHARACTERISTICS: NA

4. TYPE OF SAMPLE: Biopsy/ Excised specimen

5. PATIENT PREPARATION: NA

6. TYPE OF CONTAINER AND ADDITIVES: Plastic close container

7. REQUIRED EQUIPMENT AND REAGENT: 10% Neutral buffer formalin / Bouin’s fluid.

8. ENVIRONMENTAL AND SAFETY CONTROLS: Universal safety precautions

9. CALIBRATION PROCEDURE(METROLOGICAL TRACEBILITY): NA

10. PROCEDURE(STEPS):

• For routine histopathological examination specimen should be sliced 1cm apart , put cotton soaked in 10% formalin inside it submerged in 10% buffered formalin (volume of formalin should be 10 times of the specimen) and for frozen section examination tissue should be received in normal saline.

• If the specimen is large. Cut the specimen in smaller slice with proper orientation with 1 cm thickness.

• If fixative is hemorrhagic, change the formalin.

• Keep the received specimen and respective requisition form at proper suitable place.

11. QUALITY CONTROL PROCEDURE: NA

12. INTERFERENCE: Formalin pigment.

13. PRINCIPLE OF PROCEDURE FOR CALCULATING RESULTS INCLUDING, WHERE RELEVENT, THE MEASUREMENT OF UNCERTAINITY OF MEASURED QUANTITY VALUES: NA

14. BIOLOGICAL REFERENCE INTERVALS OR CLINICAL DECISION VALUES: NA

15. REPORTABLE INTERVAL OF EXAMINATION RESULTS: NA

16. INSTRUCTION FOR DETERMINING QUANTITATIVE RESULTS WHEN RESULTS IS NOT WITHIN THE MEASUREMENT INTERVAL: NA

17. ALERT/CRITICAL VALUE, WHERE APPROPRIATE: Autolysed specimen

18. LABORATORY CLINICAL INTERPRETATION:NA

19. POTENTIAL SOURCE OF VARIATION: NA

20. REFERENCE: Histopathological laboratory techniques by Bancroft, 6th edition