Dr Piyush Tailor

Staining Procerure:

Take test and positive control slides.

Deparaffinize section by keeping in oven for 5 min And clear by 2 changes of xylene for 15 min each .

Dehydration with graded alcohol 2 change for 2 min.

Bring section to distilled water.

Place slide in jar containing 0.5% Periodic acid for 5 min.

Wash well in several changes of distilled water.

Stain with schiff’s reagent for 10-15 min.

Tap water washes for 5-10 min.

Counter stain with Harrish Hematoxylin for 1 min.

Wash in running tap water

Differentiate in 1% acid alcohol for 1-2 sec.

Wash well in running tap water.

Dehydrate in alcohol, clear in xylene, mount with DPX.