Dr Piyush Tailor

Bring the blocks on ice plate during cutting. Do rough cutting.

Trim the blocks after putting the blocks on the ice for 15 mins.

First smaller tissues are cut after that bigger tissues are cut.

Set temperature at 60c in water bath.

Insert the appropriate sharp knife (disposable) into the microtome at 45 degree angle.

Before making ribbon of section tight all the screw of microtome.

TO make the section ribbon set the thickness of about 5-7 micrometer.

The ribbon is directly transferred to water bath heated up to 5-6 degree below the melting point of wax.

Remove the creases from the ribbon and take the ribbon containing single large section or two to three sections on albumenized slide leaving preferably ¾ inch at one end for label and ½ inch on another end.

If during removing creases any damage to the section occur then ribbon should be discarded.

Give the same number on the slide as on the block.

Put the slides rack in to the incubator at 60 degree temperature for 2 hrs.

Give xyline bath for clearing for 40 mins.

Rehydrate the sections by putting the slides in to increasing alcohol concentration and lastly in water.

Now stain the sections according to standard guidelines of different types of staining procedures.