Dr Piyush Tailor

Staining Procedure:

• Take test slide on which staining has to be performed along with positive control.
• Deparaffinize section by keeping in oven for 5min and clear it with 2 changes of xylene each for 15 minute xylene.
• Dehydrate section with graded alcohol 2 changes each for 2 minutes.
• Bring section to distilled water.
• Dilute the MGG stain with buffer (1:10). Apply the stain over the slide for 5 minute.
• Remove the stain from smear.
• Dilute the Giemsa stain with tap water (1:4).
• Apply the stain over the slide for 15-20 minutes.
• Wash with tap water.
• Dehydrate in alcohol, clear in xylene, mount with DPX
• Label it properly