Dr Piyush Tailor

Staining Procedure:

Deparafinize sections by keeping in oven for 5 minute, dlear it with xylene 2 changes for 15 minute each.

Wash in tap water.

Stain nuclei by the Celestin blue for 5 minute.

Differentiate with 1% acid alcohol.

Wash well in tap water.

Stain in acid fuschin solution for 5 minutes.

Rinse in DW.

Treat with phosphomolybdic acid solution for 5 mintues.

Drain.

Stain with methyl blue solution for 2 minutes.

Rinse in DW.

Treat with 1% acetic acid for 2 minutes.

Dehydrate through alcohols.

Clear in xylene and mount in DPX.