Dr Piyush Tailor

1. Take 2 glass beaker of 500 ml capacity and add 70% acetone in first one and 80% of acetone in second beaker. put tissue cassette in first beaker for 30 minute

2. After 30 minute remove tissue bucket from first bucket and put in second bucket for 30minute.

3. Replace acetone of first bucket with 90% acetone.

4. After 30 minute remove tissue bucket from second bucket and put in first bucket of 90% acetone for 30minute.

5. Replace acetone of first bucket with 100% acetone.

6. After 30 minute remove tissue bucket from first bucket and put in second bucket of 100% acetone for 30minute.

7. After these steps tissue cassettes are kept in xylene for two change each for 1 hour.

8. Then cassettes are transferred to wax bath of histokinet.

9. Wax bath is connected with electric power; wait till temperature reach at 65 C. put bucket inside for one & half hour.

10. After 1 hour remove cassettes from it add new wax in wax bath and again keep cassettes for 1 hour. Tissue is ready for embedding.