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nchsls:c:histopathology:document:wdi_of_iron_stain

WDI FOR IRON STAIN

Take slide on which staining has to be performed along with positive control.

Bring section to distilled water.

Transfer section in to fresh solution of equal parts of 2% aqueous potassium ferrocyanide and 2% HCL for 30 minute.

Wash thoroughly in several changes of distilled water.

Counterstain with safranin for 10-15 seconds.

Wash in water, dehydrate with alcohol, clear in xylene and mount with DPX.

Dehydrate in alcohol, clear in xylene, mount with DPX

nchsls/c/histopathology/document/wdi_of_iron_stain.txt · Last modified: 2022/08/13 07:34 (external edit)