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nchsls:c:histopathology:document:wdi_of_h_e_stain-histo
H&E Staining
1. Put the section in oven for 5 minute.
2. Dewax sections in xylene giving two changes for 15 minutes.
3. Dry the section in air.
4. Put the section in85% alcohol for 2 minute.
5. Put the section in80% alcohol for 2 minute.
6. Dry the section in air.
7. Dip the section in water for 2 times.
8. Stain with Harris Hematoxylin for 1 minutes.
9. Wash well in tap water for five Minutes till sections are blue.
10. Check the slide for optimal staining under in microscope.
11. Dip in 1% acid-alcohol: 1-2 dips.
12. Wash in tap water 10 minute.
13. Stain in 1% Eosin for 30 second.
14. Wash in tap water for 1-2minutes.
15. Dry the section in air.
16. Dehydrate section by one dip in 100% Alcohol.
17. Clear in Xylene1dip, mount with DPX after.
nchsls/c/histopathology/document/wdi_of_h_e_stain-histo.txt · Last modified: 2022/08/13 07:34 (external edit)
