Dr Piyush Tailor

Staining Procedure:

Take test slide on which staining has to be performed along with positive control.

Deparaffinize section by keeping in oven for 5min and clear it with 2 changes of xylene each for 15 minute xylene.

Dehydrate section with graded alcohol 2 changes each for 2 minutes.

Bring section to distilled water.

Stain in freshly prepared 1% alcian blue for 30 minute.

Wash in tap water wash for 2 min.

Counter stain with haematoxylin for 1min

Wash in running tap water for 5min

Dehydrate in alcohol, clear in xylene, mount with DPX