Dr Piyush Tailor

Test Procedure:

Take test slide on which staining has to be performed along with positive control.

Deparaffinize section with a mixture of 2 part xylene and 1 part of ground nut oil and keep it in oven for 10 minute and clear it with 2 change of xylene each for 15minute.

Blot with filter paper until of semidry appearance.

Wash section in water for 5 minute.

Stain with carbol fuchsin for 30 minute at room temperature.

Wash in tap water and blot with filter paper.

Decolorize with 1% acid alcohol.

Wash in water.

Counter stain with 1% methylene blue for 1 dip.

Dehydrate in alcohol, clear in xylene, mount with DPX