Dr Piyush Tailor

In operation theater of our hospital 40% formaldehyde solution is provided from store of hospital.

All buffer powder like K2Hpo4 and NaH2Po4 are supplied by histopathology section on demand of OT sister in-charge.

All buffer powder is weighed according to formula for 1 liter, 5 liter and 20 liter.

OT sisters are trained for preparation of Buffered formalin solution.

Procedure for preparation 20 liter of 10% buffered formalin:

• Take 2 liter of 40% formaldehyde solution in one big container
• Add 18 liter of tap water in container containing 40% formaldehyde.
• Mix well and add 130 gm K2HPO and 80 gm NaH2PO4
• Mix well till all ingredients have dissolved.
• Store this solution in closed container for use.

Formula for preparation 1 liter of 10% buffered formalin preparation

• Take 100 ml of 40% formaldehyde solution in one container.
• Add 900 ml of tap water in container containing 40% formaldehyde.
• Mix well and add 6.5 gm K2HPO and 4 gm NaH2PO4.
• Mix well till all ingredients have dissolved.
• Store this solution in closed container for use.

Formula for preparation 5 liter of 10% buffered formalin preparation

• Take 500 ml of 40% formaldehyde solution in one big container
• Add 4500 ml of tap water in container containing 40% formaldehyde.
• Mix well and add 32.5 gm K2HPO and 20 gm NaH2PO4
• Mix well till all ingredients have dissolved.
• Store this solution in closed container for use.