Dr Piyush Tailor

• Bring the reagent to the room temperature.
• Prewarm the reagent to 370 C.
• Add 0.1 ml of plasma to the test tube and place test tube and place the tube in water bath for 3-5 mins at 370 C.
• Forcibly add 0.2 ml of LYOPLASTIN reagent and simultaneously start the stop watch. Shake tube to mix Content.
• Gently tilt the tube and forth and stop the stopwatch as soon as the first fibrin strand is visible and the gel/clot formation begins.
• Record the time in seconds.

• Bring the required reagent to the room temperature.
• Prewarm required volume of reagents (CELIN-SE & CaCl2) for immediate use in clean & dry test tube at 370 C
• Pipette 100 ųl of test plasma & control into test cuvette at 370 C.
• Pipette 100ųl of the prewarmed CELIN-SE into test & control cuvette.
• Mix well & incubate at 370C for 10 min.
• Forcibly pipette 100ųl of prewarmed CaCl2 into test & control cuvette. Start stop watch simultaneously. Shake the tube briefly to mix the content, keep at 37 degree C for 20 seconds.
• Following 20 seconds incubation, remove the tube gently tilt back and forth until gel clot forms, stop the watch & record the clotting time in seconds.