Dr Piyush Tailor

START:
1. Switch on main power supply
2. Switch on UPS
3. Switchthe instrument on by pressing the button at the rear of the instrument.
4. Wait during initialization.
5. Switch on the printer.
6. After that login to application , to login into application select username and enter password.
7. (Optional) Select Disable Auto Loader if you want to work in STAT mode (runs a manual sampling) only. (Start Rack: runs an automatic sampling).
8. Select the following options: ■ Erase Worklist ■ Reset AutoNumbering ■ Archive Reports .(This step can only be performed when you start the instrument for the first time of the day. The first two options are optional, depending on your laboratory's rate of analysis. It is highly recommended to select the third option at the first startup of each day).
9. Press OK
10. After that click on start up icon for start up pass .
11. Select which cycle you want to perform.(startup/retic blank cycle)
12. Wait until the cycle is over. cycle takes about 3 minutes 30 seconds. Blank cycles (cycles without any blood specimen) are performed during the startup cycle.
13. After start up pass, instrument is ok for sample and control run.

SAMPLE PROCESSING: If start up is passed we can run the sample.
To Run the Specimen in STAT Mode-
1. Press STAT Mode to open the tube holder door.
2. Enter information about the sample.
3. Select the test to perform by pressing CBC/DIFF/RET.
4. Press CDR if you need a post-dilution on the sample.
5. Select the appropriate dilution ratio for WBC/LMNE and for RBC/PLT/HGB.
6. Press Validate in the contextual toolbar.
7. Gently mix the blood specimen.
8. Put the tube in the tube holder and close the door to start sampling.
9. When the door opens, remove the tube from the tube holder and recap it if needed.

To Run the Specimen in Rack Mode
1. Prepare your worklist.
2. If you work with tubes with barcode labels, place the tube on any rack, at any position. Make sure that the barcode label is visible for the internal barcode reader.
3. If you work with tubes with no barcode labels, place the tube on the rack and at the position specified in the worklist.
4. Place the rack on the rack loader.
5. Press Start Rack.

SHUTDOWN:- 1. It is highly recommended to perform a shutdown cycle before switching off the instrument.
2. Press Quit in the contextual toolbar.
3. Press shutdown icon to perform a Shutdown cycle manually.
4. Wait while the instrument shuts down. When the instrument is ready to be switched off, the following message appears on your screen: “It's now safe to turn off your computer.
5. Switch off the instrument.

1.When a reagent “Low Level” alarm is displayed, press Check.
2. Press the icon of the reagent you need to change
3.Press Edit in the contextual toolbar.
4. Enter the lot number in Lot Nb using the external barcode reader. All the fields are updated and the reagent level is set to an automatic default level.
5. Open the front door and remove the empty bottle from the reagent compartment.
6.Uncap a new reagent bottle.
7. Insert the stopper assembly tube into the new bottle and tighten the stopper assembly to ensure an adequate seal
8.Install the new reagent bottle into the reagent compartment and close the door.
9.Press Validate in the contextual toolbar. The instrument automatically starts priming the reagent.

MAINTENANCE:-

1.Concentration cleaning- to clean the counting chambers and hydraulic parts.
■ Remove the right front cover and the right-hand side panel.
• Press Concentrated Cleaning.
• Press OK. A popup window informing you to pour ABX Minoclair in each chamber is displayed.
• Use the syringe to pour 3 mL of ABX Minoclair (or diluted bleach) in each chamber.
• Press OK. A popup window is displayed.
• Use the syringe to pour 3 mL of ABX Minoclair (or diluted bleach) in the WBC/BASO chamber (last chamber on the right).
• Press OK.
• Wait for the instrument to complete the cleaning cycle.
• A concentrated cleaning takes about five minutes.

2. Miniclean Cycle
• Press Miniclean.
• Wait for the instrument to complete the cleaning cycle.
A Miniclean cycle takes about 35 seconds.

3. Autoclean Cycle
• Give the counting chambers a quick cleaning.
• Press Autoclean.
• Wait for the instrument to complete the cleaning cycle.
An Autoclean cycle takes about 90 seconds.

4. Backflush
Clean the counting chamber apertures in case of blockage.
Only a super user can perform this action.
• Press BackFlush.
• Wait for the instrument to complete the backflush cycle.
A backflush cycle takes about 35 seconds.

5. Rinse the Cytometer
Only a super user can perform this action.
The LMNE flow cell can sometimes be defective due to air bubbles and/or blockage. Rinsing the cytometer allows to fix both these issues. To do so, you need to:
• Press Rinse Cytometer.
• Wait for the instrument to complete the cytometer rinsing cycle.
A cytometer rinsing cycle takes about 90 seconds.