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nchsls:c:cytopathology:document10:fluid_processing
FLUID PROCESSING
- PURPOSE: To prepare the slide from received body fluid, sputum and BAL fluid
- STAFF RESPONSIBLE: Senior technician.
- PRINCIPLE: Sedimentation
- REAGENT: Glacial acetic acid
- PERFORMANCE SPECIFICATION: 2000rpm for 10 mins
- Linearity: NA
- Precision and accuracy: NA
- Sensitivity/specificity: NA
- SAMPLE: Body fluid, BAL, Urine,Bladder wash, CSF
- TYPE OF CONTAINER: Wide mouth sterile container
- EQUIPMENT AND REAGENT USED: Conventional centrifuge, cytotek centrifuge,slides, applicator, WBC diluting, specimen, chamber holer, filter paper.
- CALIBRATION PROCEDURE: Calibration of centrifuge is done regularly.
- PROCEDURE (STEPS):
- Conventional centrifuge
- Take the fluid into the test tube.
- In case of Hemorrhagic fluid, add equal volume of glacial acetic acid to the fluid.
- Centrifuge it at 2000 rpm for 10 min.
- Remove the supernatant, mix the sediment with the help of vortex mixture, take one drop on slide, prepare the smears.
- QUALITY CONTROL PROCEDURE: When new
- REFERENCE INTERVALS: NA
- TAT FOR TEST: Immediately processed
- LABORATORY INTERPRETATION: NA
- ALERT CRITICAL VALUE: NA
- SAFETY PRECAUTION: Universal safety precaution (Biosafety manual).
- VALIDATION OF RESULTS TO BE DONE BY: By authorized signatories &Assistant Technical manager.
- REVIEW, RECORDS & RECOMMENDATION: As per ISO:15189 Guidelines Clause 4.13 & NABL 112
- Reference:
- Theory & practice of Histological Techniques, 4th edition, 1996, John D. Bancroft
- Koss diagnostic Cytology & its Histopathologic bases, 5th edition, volume 2
nchsls/c/cytopathology/document10/fluid_processing.txt · Last modified: 2022/08/13 07:34 (external edit)
