Dr Piyush Tailor

After preparing the smears

First dry it in air for 5 minutes (Dry Fixation)

Then fix it in methanol for 10 minutes.

Dilute the MGG stain with buffer (1:10).

Apply the stain over the smear for 5 minute.

Remove the stain from smear.

Dilute the Giemsa stain with tap water (1:4).

Apply the stain over the smears for 15-20 minutes.

Wash with tap water.

Dry the smears and mount with D.P.X.

Label it properly