Dr Piyush Tailor

1.PURPOSE To prepare the slide from received sputum

2.STAFF RESPONSIBLE Senior technician.

3.PRINCIPLE Spread the representative part of sputum on glass slide, with circular movement

4.REAGENT NA

5.PERFORMANCE SPECIFICATION NA

6.SAMPLE Sputum

7.TYPE OF CONTAINER Wide mouth sterile container

8.EQUIPMENT AND REAGENT USED

• slides
• applicato

9.CALIBRATION PROCEDURE NA

10. PROCEDURE (STEPS)

• From sputum take the bloody, discoloured & solid particle not larger than the size of pea on each of four plain slides.
• With a clean glass slide crush the particle of sputum on each of the four slides, using the rotary motion.
• Spread the material evenly over the slide so that final preparation is only slightly thicker than a blood smear.
• Prepare 4 wet fix smears for pap & H E, 1 dry fix for MGG stain.

11. QUALITY CONTROL PROCEDURE NA

13. REFERENCE INTERVALS NA

14. TAT FOR TEST Immediately processed

15. LABORATORY INTERPRETATION NA

16. ALERT CRITICAL VALUE NA

17.SAFETY PRECAUTION Universal safety precaution (Biosafety manual).

18.VALIDATION OF RESULTS TO BE DONE BY By authorized signatories &Assistant Technical manager.

19.REVIEW, RECORDS & RECOMMENDATION As per ISO:15189 Guidelines Clause 4.13 & NABL 112

20.Reference Koss diagnostic cytology & its Histopathologic bases, 5th edition, volume 2