Dr Piyush Tailor

• Take 10 ml of urine in a test tube.
• Heat the upper part of the test tube.
• If protein is present in specimen, it will precipitate on heating at around 40 - 60º C
• Again continue heating the specimen till boiling level at 1000 C.
• If Bence-Jones protein is present in the specimen precipitate will dissolve (disappear) on boiling temperature.
• Allow the sample to cool.
• Precipitate will reappear on cooling.

• Ammonium sulphate test
  • Take 5 ml of urine in a test tube.
  • Add 2.8 gm of ammonium sulphate powder in test tube. Mix well. This became saturated solution of ammonium sulphate.
  • Centrifuge at 2500 rpm for 5 min.
  • Observe supernatant.
  • Supernatant is clear / plasma cleared- myoglobin is absent in urine
  • Supernatant is pink / reddish in colour- myoglobin is present in urine

Principle: Ammonium sulphate precipitate hemoglobin and after centrifugation precipitate are settled down and supernatant become clear in appearance.

*Ether test

• Take 2 ml of urine in a test tube.
• Add 2 ml of ether in the test tube.
• Mix the mixture well and observe.
• If the solution became clear – chyle is present in urine sample
• If the solution remain turbid/ urine does not mix with ether – chyle is absent in urine sample

Principle:

• Ether is fat solvent, so chylomicrons get soluble when mixed with ether. In chyluria urine contain high amount of chylomicrons which gave it whitish colour grossly.
• In ether test equal amount of ether and urine is mixed.
• If mixture contain chylomicrons it get dissolved in ether and mixture become clear in appearance.