3 levels of calibrator ,in ready to use form ,liquid kept at 2 to 8 degree celcius temperature
for each level 7 allicorte of 300 microliter was made and parafilmed ,barcode label sticed and kept in urine micro container and that were kept in box and labelled as fresh calibrator cupand put in deep fridge no 16
Left over sample, which was used for calibration , was kept in original bottle of calibrator