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nchsls:c:biochemistry:document:sample_preparation_for_tibc

Sample preparation for TIBC

  1. Write sample Id on rough part of ependroff cup according to worklist.
  2. Dispense 400 microliter iron standard(500 microgram/dl) in each ependroff cup.
  3. Add 200 microliter sample in ependroff cup after matching sample ID written on ependroff with vacutte.
  4. Incubate for 10 minutes.
  5. Add 100 mg Mgco3 ependroff cup(stored in refrigerator).
  6. Incubate for 20 minutes.
  7. Centrifuge cups at 4500 RPM for 10 minutes after incubation.
  8. After centrifugation , seperate supernatant in another ependroff cup.
  9. Put cups as sample in Erba XL-640.
nchsls/c/biochemistry/document/sample_preparation_for_tibc.txt · Last modified: 2022/08/13 07:34 (external edit)