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nchsls:c:biochemistry:document:sample_preparation_for_hdl
Preparation of Aliquots
- Write sample id on ependroff cups with permanent marker pen on the rough surface of ependroff cup.
- Arrange them in ascending order in the rack.
- Aspirate serum from the centrifuged vacutte with the help of 200 uL pipette.
- Dispense the serum into the respective sample id ependroff cup.
- Recap the ependroff cup as soon as possible after dispensing serum.
- Put ependroff cups with cap in refrigerator(2-8 ') if analysis is delayed.
Sample preparation for HDL
- Print examination wise worklist for HDL cholesterol(CHOH).
- Write sample Id on rough part of ependroff cup according to worklist.
- Dispense 20 microliter HDL reagent in each ependroff cup.
- Add 200 microliter sample in ependroff cup after matching sample ID written on ependroff with vacutte.
- Incubate sample and reagent mixture for 20 minutes.
- Centrifuge cups at 4500 RPM for 10 minutes after incubation.
- After centrifugation , Put cups as sample in Erba XL-640.
nchsls/c/biochemistry/document/sample_preparation_for_hdl.txt · Last modified: 2022/08/13 07:34 (external edit)
