1. 3 level of calibrator level-1 , level-2, and level-3
2. It comes in frozen state and ready to use form
3. Each level contain 2 ml of calibrator
4. From main bottle 4 cup was made of 0.5 ml from each level
5. This cup was capped and parafilm done
6. Barcode label was sticked on lower part which has mentioned Name lot no and level
7. kept in urine micro-container and labelled as fresh cup
8. For next time calibration use this cup
9. After calibration retained calibrator was kept in original bottle in which it actually came and it was labelled as used cup
10. This retained calibrator will be used as a quality control sample in future
11. All this kept in box
12. This box kept in deep friedge no-16
13. After each calibration kept retained calibrator in its original bottle
14. For calibration do not use sample from its original bottle ,use labelled parafilmed cup
15. tropi,PCT,proBNP mean and sd data
16. Kit literature of hS TROP I test

lot_verification_of_vitros-3600.ods