nchsls:c:biochemistry:document:how_to_make_d-dimer_internal_quality_control
  • We Have ready made, lyophilized, dry powder formed calibrator with two different concentration of D-DIMER. See photograph
  1. 3200 ng/ml
  2. 5420 ng/ml
  • We are using Normal Saline (NS) for reconstitution purpose.
  • For reconstitute the Calibrator with 3200 ng/ml concentration, add 1 ML of NS into dry calibrator. So resulted concentration will be of 3200 ng/ml.
  • For reconstitute the Calibrator with 5420 ng/ml concentration, add 1700 uL of NS into dry calibrator. So resulted concentration will be of 3200 ng/ml.
  • This is our stock solution.
  • How to make QC:-
  • We decided to make QC of 800 ng/ml concentration from this stock solution.
  • For making such QC, take 800 uL of stock solution.
  • Add 2200 uL of Normal Saline.
  • Total volume will be of 3000 uL (3 ML).
  • From this 3 ml, Make cups of 200 uL.
  • 15 cups will be made.
  • Use these cups as QC.
nchsls/c/biochemistry/document/how_to_make_d-dimer_internal_quality_control.txt · Last modified: 2022/08/13 07:34 (external edit)