Dr Piyush Tailor

1. Take 5 ml Qc water(DI water of 0.00 microsiemens/cm) in volumetric flask.
2. Gently transfer water in Randox Human assay level 2 and level 3 bottle.Pour last drop of water by simply rotate flask over edge of bottle.Do not beat the flask over bottle.
3. Mix it by gentle inversion and eversion.
4. Put it at room temperature for 30 minute and watch for all powder particle has been dissolved.
5. Fill 10 ependroff cup of 500 microliter.and seal with it parafilm.

1. Make low and high lipase serum pool as quality control samples.
2. For low lipase serum pool , collect leftover serum from OPD samples.
3. Find serum lipase > 20 IU/L for 3-4 days.
4. Collect sample in 10 ml vacutte everyday.kept vacutte in deep freeze.
5. After approximately 10 ml serum collected,Centrifuge vacutte at 3000 RPM for 10 minutes.
6. Fill 100 microliter in ependroff cup. Make such 90 such ependroff cups.

1. wash 50 ml flask.
2. take 500 microliter of calibrator¹⁾ in that flask.
3. take DI water in flask upto the mark and wrap parafilm over mouth of flask.
4. mix it well.
5. wash 50 ml bottle and fill this diluted calibrators in flask in washed bottle.
6. make approximately 100 cup of MPR calibratorwith 500 microliter volume and airtight cup with parafilm.
7. put all cups in deep fridge (-40^oc).

Note: while making cup of 500 microliter shack 50 ml bottle with diluted calibrators intermittantly.

1. Make low and high ADA pool as quality control samples.
2. Find ADA < 15 and ADA > 15 for 3-4 days.
3. Collect sample in 10 ml vacutte everyday.kept vacutte in deep freeze.
4. After approximately 10 ml serum collected,Centrifuge vacutte at 3000 RPM for 10 minutes.
5. Fill 100 microliter in ependroff cup. Make such 90 such ependroff cups.

1. Take out normal and abnormal quality control.
2. Program Qc sample as any other sample in erba XL-640.See How to Run samples in Erba XL-640.
3. For convinience Normal Qc=5 and Abnormal Qc=8.
4. Format for giving Qc ID=5YYMMDDHH.²⁾
5. For example,If you are doing Qc 9 a.m. in morning on 13th june,2014 then give Id=514061309 and 814061309.
6. After Qc sample run completed , Export results from Erba XL-640 and Import QC results to LIS

1. Open 10.207.3.240.
2. Enter user name and password.
3. Click on 'Quality—>LJ chart/insert/comment Qc'.Folowing window open.
4. Select Day , Eqipment name and tick all examination.Folowing window open.
5. Black line in middle=Mean , Green line=1 SD , Blue line=2SD , Red line=3SD.
6. Follow 1(3S) and 2(2S) rules for rejecting a run/accepting a run.
7. Following is general guideline to identify, correct and prevent nonconformance.
   1. 1(3S): Random and Systemic error
   2. 2(2S): Systemic error
   3. Random error: error in precision
   4. Systemic error: error in accuracy
8. Action taken when 1(3S) or 2(2S) rule violeted

1. Overlap new and old lot Human assay Quality control sera for minimun 20 days for 3 times a day.
2. After 20 days,Sort data for new Qc in Excel sheet.