 | Dr Piyush B. Tailor |
| Be Human - Be Part of Change |
*Home
*Lectures
- Model Questions
- Question Papers
- Social Media
- About
Sidebar
nchsls:c:biochemistry:document:hb_a_hb_f_hb_s_control_s
How to prepare HbA ,HBS and HBF control
For HBF Control preparation
- Collect about 2-4 ml cord blood in plastic bottle containing 2 ml CPDA
- Alternatively add washed RBC of new born EDTA sample in 2 ml CPDA bottle till total volume become 4 ml approximately. Preserve at 2-8'C till required amount is collected.
- Use washed RBC to prevent mismatch agglutination in pooled RBC
- Wash with saline twice in 4 ml EDTA vacumm tube. Remove Saline part
- After washing,Add Preservative CPDA in washed RBCs in 1:1 ratio.
- Prepare aliquots of 50 ul.
- Label and store at 2-8'C.
For HBA+HbS Control preparation
- Add washed RBC of HbA or HbS or HbA+HBS EDTA sample in 2 ml CPDA bottle till total volume become 4 ml approximately.
- Use washed RBC to prevent mismatch agglutination in pooled RBC
- Wash with saline twice in 4 ml EDTA vacumm tube. Remove Saline part
- After washing,Add Preservative CPDA in washed RBCs in 1:1 ratio.
- Prepare aliquots of 50 ul.
- Label and store at 2-8'C.
For HBA+HbS+HbF Control preparation
- Mixed HBA+HbS and HbF control in equal amount from above.
- Prepare aliquots of 50 ul.
- Label and store at 2-8'C.
nchsls/c/biochemistry/document/hb_a_hb_f_hb_s_control_s.txt · Last modified: 2022/08/13 07:34 (external edit)
