Dr Piyush Tailor

1. Take approx. 600 ml of DI water in 2 liter flask
2. Add 10.2 gm Tris base
3. Add 3.2 gm boric acid
4. Add 0.2 gm EDTA powder
5. Mix properly with stirrer and do upto 2 liter
6. Measure the Ph of final buffer and it would be 8.6