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nchsls:c:biochemistry:document:haemolysate_solution

Hemolysate

  1. 90 ml TEB Buffer (Half diluted).
  2. 2 ml Triton x -100
  3. 20 mg KCN
  4. 8 ml 0.1 M EDTA
  5. Mix well, Preserve it in fridge.

Acrylamide Hemolysate

  1. Take 1 gm acrylamide
  2. Add 10 ml hemolysate.
  3. Allow acrylamide to dissolve
  4. add 10 mg APS
  5. add 10 ul TEMED
  6. close lid
  7. allow polymerization for 30 minutes
nchsls/c/biochemistry/document/haemolysate_solution.txt · Last modified: 2022/08/13 07:34 (external edit)