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haemolysate_solution
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nchsls:c:biochemistry:document:haemolysate_solution
Hemolysate
90 ml TEB Buffer (Half diluted).
2 ml Triton x -100
20 mg KCN
8 ml 0.1 M EDTA
Mix well, Preserve it in fridge.
Acrylamide Hemolysate
Take 1 gm acrylamide
Add 10 ml hemolysate.
Allow acrylamide to dissolve
add 10 mg APS
add 10 ul TEMED
close lid
allow polymerization for 30 minutes
nchsls/c/biochemistry/document/haemolysate_solution.txt
· Last modified: 2022/08/13 07:34 (external edit)
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