1. 3 level of calibrator ,frozen powered form
2. keep at room temperature for 10-15 min
3. reconstitute with 1 ml of QC water
4. Make 5 cup of 200 microliter of each level
5. 1 cup of each l;evel will be used for calibration
6. Remained 4 cup of each calibrator label it and barcode it and place in a urine microcontained and label those as a fresh cup and use this for next time calibration
7. retained sample of calibration will be kept in its original bottle ,parafilmed it and place in urine microcontained and label of used cup
8. Kept all these cup used as well as fresh in friedge no-16
9. ft3_gem1021_ww_en_i_12_1.pdf[kit literature of FREE T3 ]

lot_verification_of_vitros-3600.ods