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nchsls:c:biochemistry:document:d_dimer
kit literature
D-DIMER Calibration with linearity establishment
- lyophilized D-DIMER calibrator bottle is reconstituted with 1 ml(1 gm qc water) DI water, incubated for 10 to 15 minutes with periodic shaking until solutes becomes completely dissolved.
- 200 Ul of the mixture(reconstituted calibrator) is added with 200 Ul of Di water.
- from there again 200 Ul of solution is added to the next alliquote and 200 Ul water is added for 3 more subsequent times. (serial dilution)
- For establishing linearity a total of 6 samples are required.
- 1 sample is the plain calibrator(stock d-dimer), and the other one is DI water.
- the samples are analysed in the analyser and od is plotted in the graph.
- from there the factors are extracted and feeded in the machine.
- For making an (IN use)ready to use reagent, 2000 uL of R1 is added with 500 uL of R2, which was already kept in fridge 2* to 8* C.
- special wash reagent (0.1 n NAOH) is also added for wash
Calibrator traceability certificateqdd1026.pdf
sample comparison with Coral kit and Labcare kit d-dimer_sample_comparison05_08.ods
nchsls/c/biochemistry/document/d_dimer.txt · Last modified: 2022/08/13 07:34 (external edit)
