 | Dr Piyush B. Tailor |
| Be Human - Be Part of Change |
*Home
*Lectures
- Model Questions
- Question Papers
- Social Media
- About
Sidebar
nchsls:c:biochemistry:document:current_calibration_frequency_iqc_and_eqc_policy
Frequency of calibration and IQC
| Erba XL-1000 | |||||
|---|---|---|---|---|---|
| Parameter | Calibration | IQC | Calibration Policy | Correlation Factor | EQC Policy |
| calibrator and Replicates/QC and Replicates | |||||
| prepare 200ul calibratuo cups and devide 500ul QC cups in to 200ul | |||||
| perform recalibration of same lot if qc results are out of 1SD on same side for more than 15days | |||||
| GLC | WHEN LOT CHANGEs or if calibration is older than 1 month even if lot is same | 2 LEVEL IQC at 8 AM ,3 PM , 10 PM | 3 calibrators cups X 4 time replicate and 3 IQC cups X 4 time replicate and 3 blank cups X 4 time replicate | y=ax+b | RIQAS_monthly |
| Sodium | whenever electrodes changed. | Do 2 level IQC 5 times when fluid pack refilled and 2 level IQc at 8 AM ,3 PM , 10 PM | 5 calibrator CUPS X 2 time replicate and 5 IQC cups x 2 time replicate | y=ax | RIQAS_monthly |
| Potassium | whenever electrodes changed. | Do 2 level Qc 5 times when fluid pack refilled and 2 level Qc at 8 AM ,3 PM , 10 PM | 5 calibrator CUPS X 2 time replicate and 5 QC cups x 2 time replicate | y=ax | RIQAS_monthly |
| TBIL | WHEN LOT CHANGES | 2 LEVEL IQC at 8 AM ,3 PM , 10 PM | Use freshly prepared calibrators and quality control(after one hour of reconstitution)3 calibrators cups X 4 time replicate and 3 IQC cups X 4 time replicate and 3 blank cups X 4 time replicate | y=ax+b | RIQAS_monthly |
| DBIL | WHEN LOT CHANGES | 2 LEVEL IQC at 8 AM ,3 PM , 10 PM | Use freshly prepared calibrators andUse freshly prepared calibrators and3 calibrators cups X 4 time replicate and 3 IQC cups X 4 time replicate and 3 blank cups X 4 time replicate | Use TBIL Factor achieved in calibration | RIQAS_monthly |
| ALT | WHEN LOT CHANGES | 2 LEVEL IQC at 8 AM ,3 PM , 10 PM | 3 calibrators cups X 4 time replicate and 3 IQC cups X 4 time replicate and 3 blank cups X 4 time replicate | y=ax+b | RIQAS_monthly |
| TG | WHEN LOT CHANGES or if calibration is older than 1 month even if lot is same | 2 LEVEL IQC at 8 AM ,3 PM , 10 PM | 3 calibrators cups X 4 time replicate and 3 IQC cups X 4 time replicate and 3 blank cups X 4 time replicate | y=ax+b | RIQAS_monthly |
| CHO | WHEN LOT CHANGES or if calibration is older than 1 month even if lot is same | 2 LEVEL IQC at 8 AM ,3 PM , 10 PM | 3 calibrators cups X 4 time replicate and 3 IQC cups X 4 time replicate and 3 blank cups X 4 time replicate | y=ax+b | RIQAS_monthly |
| UREA | WHEN LOT CHANGES | 2 LEVEL IQC at 8 AM ,3 PM , 10 PM | 3 calibrators cups X 4 time replicate and 3 IQC cups X 4 time replicate and 3 blank cups X 4 time replicate | y=ax+b | RIQAS_monthly |
| UA | WHEN LOT CHANGES | 2 LEVEL IQC at 8 AM ,3 PM , 10 PM | 3 calibrators cups X 4 time replicate and 3 IQC cups X 4 time replicate and 3 blank cups X 4 time replicate | y=ax+b | RIQAS_monthly |
| CKMB | Fixed Factor,adjust factor according to dilution factor when kit/protocol changed based on molar absorptivity of NADPH | KIT CONTROL WHEN BOTTLE CHANGE.2 LEVEL IQC AT 8 AM, 1 LEVEL IQC AT 3 PM, 1 LEVEL IQC AT 10 PM | NA. | Fixed Factor | |
| ALB | WHEN LOT CHANGES | 2 LEVEL IQC at 8 AM ,3 PM , 10 PM | 3 calibrators cups X 4 time replicate and 3 IQC cups X 4 time replicate and 3 blank cups X 4 time replicated | y=ax+b | RIQAS_monthly |
| ALP | WHEN LOT CHANGES | 2 LEVEL IQC at 8 AM ,3 PM ,10 PM | 3 calibrators cups X 4 time replicate and 3 IQC cups X 4 time replicate and 3 blank cups X 4 time replicate | y=ax+b | RIQAS_monthly |
| TP | WHEN LOT CHANGES | 2 LEVEL IQC at 8 AM ,3 PM , 10 PM | 3 calibrators cups X 4 time replicate and 3 IQC cups X 4 time replicate and 3 blank cups X 4 time replicate | y=ax+b | RIQAS_monthly |
| AMY | WHEN LOT CHANGES | 2 LEVEL QC at 8 AM ,3 PM , 10 PM | 3 calibrators cups X 4 time replicate and 3 QC cups X 4 time replicate and 3 blank cups X 4 time replicate | y=ax+b | RIQAS_monthly |
| CAL | When Lot changes because reagent is used up within 35 days | 2 LEVEL QC at 8 AM ,3 PM , 10 PM (In duplicate and second value is considered) | 3 calibrators cups X 4 time replicate and 3 QC cups X 4 time replicate and 3 blank cups X 4 time replicate | y=ax+b | RIQAS_monthly |
| CAL(OCPC) | Every four hourly | 2 LEVEL QC 4 Hourly and with each new batch(In duplicate and second value is considered) | 4 calibrators(HAlf cal 2, half cal3, cal 2, cal 3) cups X 2 time replicate, one blank x 2 times | y=ax+b | RIQAS_monthly |
| CR | WHEN LOT CHANGES | 2 LEVEL QC at 8 AM ,3 PM ,10 PM | 3 calibrators cups X 4 time replicate and 3 QC cups X 4 time replicate and 3 albumin(5%) blank cups X 4 time replicate | y=ax+b(Dont Use the value of blank for finding the equation) | RIQAS_monthly |
| LDH | WHEN LOT CHANGES | 2 LEVEL QC at 8 AM ,3 PM , 10 PM | 3 calibrators cups X 4 time replicate and 3 QC cups X 4 time replicate and 3 blank cups X 4 time replicate | y=ax+b | RIQAS_monthly |
| LIP | Fixed Factor(-500) | 2 level pooled sera at 8 am | NA | Literature factor=500 | |
| IRON | Calibration when lot changes | 2 Level QC with each batch | 3 calibrators cups X 4 time replicate and 3 QC cups X 4 time replicate and 3 blank cups X 4 time replicate | y=ax+b | RIQAS_monthly |
| UIBC | Calibration when lot change and along with each batch. each batch calibration is minicalibration. | 1 Level QC with each batch | 3 calibrators cups X 4 time replicate and 3 QC cups X 4 time replicate and 3 blank cups X 4 time replicate | y=ax+b | RIQAS_monthly |
| ADA | in every batch, with 60 U/ml ADA standard X 3 times replicate | Two level pooled sera in every batch | NA | QA Forum | |
| MPR | When Lot changes | two level diluted(1:100 times) calibrator sera at 8 am (In duplicate and second value is considered) | 10 Calibrators cups X 2 time replicate and 5 blank cups X 2 time replicate | y=ax | CSF_RIQAS_monthly |
| CHOH | Fixed factor(caculated from CHO factor),change it when factor of cholesterol changes | 2 LEVEL QC daily | NA | (cholesterol factor)x1.1 | RIQAS_monthly |
| CHE | whenever new 30ml bottle is reconstituted | 2 LEVEL QC AT 8 AM, 1 LEVEL QC AT 3 PM, 1 LEVEL QC AT 10 PM | 4 calibrators cups X 2 time replicate and 4 QC cups X 2 time replicate and 2 blank cups X 2 time replicate | y=ax | RIQAS_monthly |
| PHO | When Lot changes | 2 LEVEL QC at 8 AM ,3 PM , 10 PM(In duplicate and second value is considered) | 3 calibrators cups X 4 time replicate and 3 QC cups X 4 time replicate and 3 blank cups X 4 time replicate | y=ax | RIQAS_monthly |
| Dithionite Test | Qualitative test | Two level control (positive and negative) run in each batch | NA | QA Forum_monthly | |
| Hemoglobin electrophoresis-Detection of HbA, HbS & HbF | Qualitative test | Control of HbA, HbS and HbF are run with each batch | NA | QA Forum_monthly | |
| Fluid Glucose | same as serum glucose | Same as serum Glucose IQC | 3 Serum calibrators cups X 4 time replicate and 3 QC cups X 4 time replicate and 3 blank cups X 4 time replicate | y=ax | QA FORUM 6 monthly and in CSF RIQAS monthly |
| Fluid Protein (Biuret) | Same as serum protein | Same as Serum protein IQC | 3 Serum calibrators cups X 4 time replicate and 3 QC cups X 4 time replicate and 3 blank cups X 4 time replicate | y=ax | QA Forum_6 monthly |
| Fluid Protein (Pyrogallol Red) | Same as MPR | Two level pooled fluid in every batch | 10 serum Calibrators cups X 2 time replicate and 5 blank cups X 2 time replicate | y=ax | QA FORUM_6 monthly |
| Serum Ketone bodies | Qualitative Test | Two level pooled sera( Normal and Acetone spiked) Twice a day | NA | QA Forum_6 monthly | |
| Urine Ketone bodies | Qualitative Test | Same as serum ketone | NA | QA Forum_6 monthly | |
| Urine Calcium | Same as serum calcium calibration | 1 level pooled spiked Acidify urine once when test done | 3 Serum calibrators cups X 4 time replicate and 3 QC cups X 4 time replicate and 3 blank cups X 4 time replicate | y=ax | QA Forum_6 monthly |
| Urine Creatinine | Same as serum creatinine | 1 level pooled spiked urine once when test done | 3 Serum calibrators cups X 4 time replicate and 3 QC cups X 4 time replicate and 3 blank cups X 4 time replicate | y=ax | QA Forum_6 monthly |
| Urine Phosphorous | Same as serum Phosphorus | 1 level pooled spiked Acidify urine once when test done | 3 Serum calibrators cups X 4 time replicate and 3 QC cups X 4 time replicate and 3 blank cups X 4 time replicate | y=ax | QA Forum_6 monthly |
| Urine Sodium | When Lot changes | 1 level pooled spiked urine once when test done | 3 Serum calibrators cups X 4 time replicate and 3 QC cups X 4 time replicate and 3 blank cups X 4 time replicate | y=ax | QA Forum_6 monthly |
| Urine Protein (Biuret) | When Lot changes | 1 level pooled spiked urine once when test done | 3 Serum calibrators cups X 4 time replicate and 3 QC cups X 4 time replicate and 3 blank cups X 4 time replicate | y=ax | QA Forum_6 monthly |
| Urine Protein (Pyrogallol Red) | When Lot changes | 2 level diluted calibrator once a day | 10 serum Calibrators cups X 2 time replicate and 5 blank cups X 2 time replicate | y=ax | QA Forum_6 monthly |
| Urine Uric Acid (Uricase POD) | same as serum Uric Acid | 1 level pooled spiked basic urine once when test done | 3 Serum calibrators cups X 4 time replicate and 3 QC cups X 4 time replicate and 3 blank cups X 4 time replicate | y=ax | QA Forum_monthly |
| Protein electrophoresis-Detection of M band | Qualitative Test | Three level QC run with each batch | NA | QA Forum_6 monthly | |
| CK(IFCC at 37) | When lot changes | 2 LEVEL QC AT 8 AM, 1 LEVEL QC AT 3 PM, 1 LEVEL QC AT 10 PM | 1 kit standard cup and 1 blank cup 5times replicated | y=ax+b | RIQAS monthly |
| CRP(turbidimetry) | When lot changed | 2 level in-house pool in morning 8 am | serial dilution of kit standard how_to_do_serial_dilution_of_crp_standard.ods 5 cups with 4 replicates | y=ax+b | QA Forum 6 monthly |
| DDIMER(turbidimetry) | When lot changed | 1 level diluted kit standard in morning 8 am | serial dilution of kit standard 5 cups with 1replicatehow_to_do_serial_dilution_of_ddimer_standard.ods | y=ax | QA Forum 6 monthly |
| Total 25-OH Vit-D(ECLIA) | When lot changed | IQC weekly one level | Two level calibrator freeze dried form reconstitute with 1ml qc water | Autocalibrated | RIQAS monthly |
| Ferritin | When lot changed or Calibration expired | IQC weekly one level | Three level calibrator freeze dried form reconstitute with 1ml qc water | Autocalibrated | RIQAS monthly |
| Procalcitonin | When lot changed or Calibration expired | IQC weekly one level | Two level calibrator ready to use(Frozen sample) | Autocalibrated | QA Forum 6 monthly |
| TSH | When lot changed or Calibration expired | 2 level IQC daily at 8AM, 1 level at 3 PM, 1 level at 10 PM | Three level calibrator ready to use | Autocalibrated | RIQAS monthly |
| FT3 | When lot changed or Calibration expired | IQC weekly one level | Three level calibrator freeze dried form reconstitute with 1ml qc water | Autocalibrated | RIQAS monthly |
| FT4 | When lot changed or Calibration expired | IQC weekly one level | Three level calibrator freeze dried form reconstitute with 1ml qc water | Autocalibrated | RIQAS monthly |
| LH | When lot changed or Calibration expired | IQC weekly one level | Two level calibrator ready to use(Frozen sample) | Autocalibrated | RIQAS monthly |
| FSH | When lot changed or Calibration expired | IQC weekly one level | Two level calibrator ready to use(Frozen sample) | Autocalibrated | RIQAS monthly |
| Prolactin | When lot changed or Calibration expired | IQC weekly one level | Two level calibrator freeze dried form reconstitute with 1ml qc water | Autocalibrated | RIQAS monthly |
| NTProBNP2 | When lot changed or Calibration expired | IQC weekly one level | Three level calibrator ready to use | Autocalibrated | Not done |
| HsTnI | When lot changed or Calibration expired | IQC weekly one level | Three level calibrator ready to use | Autocalibrated | QA Forum 6 monthly |
| B-HCG | When lot changed or Calibration expired | IQC weekly one level | Three level calibrator freeze dried form reconstitute with 1ml qc water | Autocalibrated | RIQAS monthly |
| Vit B12 | When lot changed or Calibration expired | Not done because no sample received | Two level calibrator ready to use(Frozen sample) | Autocalibrated | RIQAS monthly |
| CoV2G | When lot changed or Calibration expired | Not done | Single level calibrator ready to use | Autocalibrated | Not done |
| Lactate | One point autocalibration half hourly, two point autocalibration at 2 hourly | one level IQC daily | One point autocalibration half hourly, two point autocalibration at 2 hourly | - | Not done |
| HCO3 | One point autocalibration half hourly, two point autocalibration at 2 hourly | one level IQC daily | One point autocalibration half hourly, two point autocalibration at 2 hourly | - | Not done |
| pH | One point autocalibration half hourly, two point autocalibration at 2 hourly | one level IQC daily | One point autocalibration half hourly, two point autocalibration at 2 hourly | - | Not done |
| pCO2 | One point autocalibration half hourly, two point autocalibration at 2 hourly | one level IQC daily | One point autocalibration half hourly, two point autocalibration at 2 hourly | - | Not done |
| pO2 | One point autocalibration half hourly, two point autocalibration at 2 hourly | one level IQC daily | One point autocalibration half hourly, two point autocalibration at 2 hourly | - | Not done |
| HbA1C | Weekly and When buffer changed | 2 level IQC from in house pool with each batch. | Two level calibrator freeze dried form reconstitute with 4ml qc water | - | QA Forum 6 monthly |
| Erba Chem-5 Plus V2 | |||||
|---|---|---|---|---|---|
| Parameter | Calibration | IQC | |||
| When in use(Erba XL-640 breakdown) | When not in use | When in use(Erba XL-640 breakdown) | When not in use | ||
| GLC | Every batch | Every month before External QC and when lot changes | 2 level QC With Every batch | 2 level QC every Sunday | y=ax |
| BIL | when lot changes | Every month before External QC and when lot changes | 2 level QC 3 times a day | 2 level QC every sunday | y=ax |
| CR | when lot changes | Every month before External QC and when lot changes | 2 level QC 3 times a day | 2 level QC every sunday | y=ax |
| ALT | when lot changes | Every month before External QC and when lot changes | 2 level QC 3 times a day | 2 level QC every sunday | y=ax |
| ADA | in every batch | Not applicable | Two level pooled sera in every batch | Not applicable |
nchsls/c/biochemistry/document/current_calibration_frequency_iqc_and_eqc_policy.txt · Last modified: 2022/08/13 07:34 (external edit)
