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nchsls:c:biochemistry:document:creatine
Creatinine
| Procedure for Creatinine measurement | nchsls:c:biochemistry:document:examination_procedure:LDH | Electroinc-only document | Edition (1) 27-01-2014 |
| Electronic copy of the document is stored at NCHSLS server, NCHS, Surat |
| Electronic copy of the document may be printed. However, It should be destroyed as early as possible after use, to ensure that only latest documentation is used every time. |
| Creatinine Reference literature 1 |
| Creatinine Reference literature 2 |
| Creatinine Reference literature 3 |
purpose of the examination
The examination in serum and plasma is used mainly for diagnosis and management of renal disorders. Urine creatinine is frequently used as surrogate indicator to urine output
principle and method of the procedure used for examinations
Creatinine reacts with alkaline picrate to form red colored complex. After an incubation period, increase in absorbance between two time points is measured at 490-540 nm (psudo-first order kinetics).The incubation period and time points for measurement of absorbance are chosen to decrease interference from other compounds reacting with alkaline picrate with different kinetics.
performance characteristics
Linearity = 0-20[mg/dl] any one interval |Creatinine linearity check|
Within Batch Precision = <7.5[% CV]
Between Batch Precision(IQC) = <10[%cv]
EQA Peer Precision = <10[%cv]
Measuring_Interval = 0-40[mg/dl]
Trueness_of_Measurement = >40[TS]
Analytical_Sensitivity = 0.0315[A/mg/dl]
type of sample
patient preparation
type of container and additives
required equipment and reagents
environmental and safety controls
calibration procedures (metrological traceability)
procedural steps
XL-640 Filter 340 nm
Temperature 37 degree C
Method Rate-A
Step-1 200 microliter R1
Step-2 10 microliter Sample
Step-3 M1S=0 M1E=0 M2S=3 M2E=8
Step-4 Reagent Absorbance Max=0
Step-5 Reagent Absorbance Min=0
Step-6 Reaction Absorbance limit=0
Step-7 Technical Min=0
Step-8 Technical Max=1000
Step-9 Linearity limit%=0
Step-10 Delta Abs/min%=0
Step-11 Reaction direction=Increasing
quality control procedures
Internal Qc 3 times a day at 8 AM , 3 PM , 10 PM.
External Qc once in a month
interferences (e.g. lipaemia, haemolysis, bilirubinemia, drugs) and cross reactions
More than 400[mg/dl Hemoglobin]
More than 30[mg/dl Total Bilirubin]
More than 12[mg/dl ascorbate]
More than 700[mg/dl Triglyceride]
principle of procedure for calculating results including, where relevant, the measurement uncertainty of measured quantity values
biological reference intervals or clinical decision values
reportable interval of examination results
instructions for determining quantitative results when a result is not within the measurement interval
alert/critical values, where appropriate
laboratory clinical interpretation
potential sources of variation
references
nchsls/c/biochemistry/document/creatine.txt · Last modified: 2022/08/13 07:34 (external edit)
