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nchsls:c:biochemistry:document:calibration_and_quality_control_frequency
Frequency of calibration and IQC
| Erba XL-640 | ||||||
|---|---|---|---|---|---|---|
| Parameter | Calibration | IQC | Calibration Policy | Correlation Factor | EQC Policy | |
| calibrator and Replicates/QC and Replicates | ||||||
| prepare 200ul calibratuo cups and devide 500ul QC cups in to 200ul | ||||||
| perform recalibration of same lot if qc results are out of 1SD on same side for more than 15days | ||||||
| GLC | WHEN LOT CHANGEs or if calibration is older than 1 month even if lot is same | 2 LEVEL QC at 8 AM ,3 PM , 10 PM | 3 calibrators cups X 4 time replicate and 3 QC cups X 4 time replicate and 3 blank cups X 4 time replicate | y=ax | RIQAS_monthly | |
| Sodium, Potassium | Two level when batch is started, one level with each sample(Inbuilt) | Do 2 level Qc 5 times when fluid pack refilled and 2 level Qc at 8 AM ,3 PM , 10 PM | 3 calibrator CUPS of 1 ml X 5 times replicate and 3 QC cups of 1ml x 5 times replicate. After running the cups do check that around 500 ul sample is left in the cups which can be reused later. the Due to difference in expected dimension of HITACHI cup and real dimension of eppendorf cup, aspiration of 70ul is error prone if residual volume is less than 400 ul | RIQAS_monthly | Why in measurement of electrolyte do we take more than 400 ul serum for doing replication ? hitachi_cups.odt | |
| TBIL | WHEN LOT CHANGES | 2 LEVEL QC at 8 AM ,3 PM , 10 PM | Use freshly prepared calibrators and quality control(after one hour of reconstitution)3 calibrators cups X 4 time replicate and 3 QC cups X 4 time replicate and 3 blank cups X 4 time replicate | y=ax | RIQAS_monthly | |
| DBIL | WHEN LOT CHANGES | 2 LEVEL QC at 8 AM ,3 PM , 10 PM | Use freshly prepared calibrators andUse freshly prepared calibrators and3 calibrators cups X 4 time replicate and 3 QC cups X 4 time replicate and 3 blank cups X 4 time replicate | y=ax | RIQAS_monthly | |
| ALT | WHEN LOT CHANGES | 2 LEVEL QC at 8 AM ,3 PM , 10 PM | 3 calibrators cups X 4 time replicate and 3 QC cups X 4 time replicate and 3 blank cups X 4 time replicate | y=ax | RIQAS_monthly | |
| TG | WHEN LOT CHANGES or if calibration is older than 1 month even if lot is same | 2 LEVEL QC at 8 AM ,3 PM , 10 PM | 3 calibrators cups X 4 time replicate and 3 QC cups X 4 time replicate and 3 blank cups X 4 time replicate | y=ax | RIQAS_monthly | |
| CHO | WHEN LOT CHANGES or if calibration is older than 1 month even if lot is same | 2 LEVEL QC at 8 AM ,3 PM , 10 PM | 3 calibrators cups X 4 time replicate and 3 QC cups X 4 time replicate and 3 blank cups X 4 time replicate | y=ax | RIQAS_monthly | |
| UREA | WHEN LOT CHANGES | 2 LEVEL QC at 8 AM ,3 PM , 10 PM | 3 calibrators cups X 4 time replicate and 3 QC cups X 4 time replicate and 3 blank cups X 4 time replicate | y=ax | RIQAS_monthly | |
| UA | WHEN LOT CHANGES | 2 LEVEL QC at 8 AM ,3 PM , 10 PM | 3 calibrators cups X 4 time replicate and 3 QC cups X 4 time replicate and 3 blank cups X 4 time replicate | y=ax | RIQAS_monthly | |
| CKMB | Fixed Factor,adjust factor according to dilution factor when kit/protocol changed based on molar absorptivity of NADPH | KIT CONTROL WHEN BOTTLE CHANGE. | when 30 ml bottle will reconstituted. | Fixed Factor | ||
| ALB | WHEN LOT CHANGES | 2 LEVEL QC at 8 AM ,3 PM , 10 PM | 3 calibrators cups X 4 time replicate and 3 QC cups X 4 time replicate and 3 blank cups X 4 time replicated | y=ax | RIQAS_monthly | |
| ALP | WHEN LOT CHANGES | 2 LEVEL QC at 8 AM ,3 PM ,10 PM | 3 calibrators cups X 4 time replicate and 3 QC cups X 4 time replicate and 3 blank cups X 4 time replicate | y=ax | RIQAS_monthly | |
| TP | WHEN LOT CHANGES | 2 LEVEL QC at 8 AM ,3 PM , 10 PM | 3 calibrators cups X 4 time replicate and 3 QC cups X 4 time replicate and 3 blank cups X 4 time replicate | y=ax | RIQAS_monthly | |
| AMY | WHEN LOT CHANGES | 2 LEVEL QC at 8 AM ,3 PM , 10 PM | 3 calibrators cups X 4 time replicate and 3 QC cups X 4 time replicate and 3 blank cups X 4 time replicate | y=ax | RIQAS_monthly | |
| CAL | When Lot changes because reagent is used up within 35 days | 2 LEVEL QC at 8 AM ,3 PM , 10 PM (In duplicate and second value is considered) | 3 calibrators cups X 4 time replicate and 3 QC cups X 4 time replicate and 3 blank cups X 4 time replicate | y=ax | RIQAS_monthly | |
| CR | WHEN LOT CHANGES | 2 LEVEL QC at 8 AM ,3 PM ,10 PM | 3 calibrators cups X 4 time replicate and 3 QC cups X 4 time replicate and 3 albumin(5%) blank cups X 4 time replicate | y=ax+b | RIQAS_monthly | |
| LDH | WHEN LOT CHANGES | 2 LEVEL QC at 8 AM ,3 PM , 10 PM | 3 calibrators cups X 4 time replicate and 3 QC cups X 4 time replicate and 3 blank cups X 4 time replicate | y=ax | RIQAS_monthly | |
| LIP | Fixed Factor(-500) | 2 level pooled sera at 8 am | NA | Literature factor=500 | ||
| IRON | Calibration when lot change and along with each batch | 2 Level QC with each batch | 3 calibrators cups X 4 time replicate and 3 QC cups X 4 time replicate and 3 blank cups X 4 time replicate | y=ax | RIQAS_monthly | |
| UIBC | Calibration when lot change and along with each batch | 2 Level QC with each batch | 3 calibrators cups X 4 time replicate and 3 QC cups X 4 time replicate and 3 blank cups X 4 time replicate | y=ax | RIQAS_monthly | |
| ADA | in every batch, with 60 U/ml ADA standard X 3 times replicate | Two level pooled sera in every batch | NA | QA Forum | ||
| MPR | When Lot changes | two level diluted(1:100 times) calibrator sera at 8 am (In duplicate and second value is considered) | 10 Calibrators cups X 2 time replicate and 5 blank cups X 2 time replicate | y=ax | RIQAS_monthly | |
| CHOH | Fixed factor(caculated from CHO factor),change it when factor of cholesterol changes | 2 LEVEL QC EVERY MORNING AND EVENING | NA | (cholesterol factor)x1.1 | RIQAS_monthly | |
| CHE | whenever new 30ml bottle is reconstituted | RIQAS_monthly | ||||
| CK | When Lot changes | 1 level QC every morning | 3 calibrators cups X 4 time replicate and 3 QC cups X 4 time replicate and 3 blank cups X 4 time replicate | y=ax | RIQAS_monthly | |
| PHO | When Lot changes | 2 LEVEL QC at 8 AM ,3 PM , 10 PM(In duplicate and second value is considered) | 3 calibrators cups X 4 time replicate and 3 QC cups X 4 time replicate and 3 blank cups X 4 time replicate | y=ax | RIQAS_monthly | |
| Dithionite Test | Qualitative test | Two level control (positive and negative) run in each batch | NA | RIQAS_monthly | ||
| Hemoglobin electrophoresis-Detection of HbA, HbS & HbF | Control of HbS and HbF are run with each batch | NA | ||||
| Fluid Glucose | When Lot changes | Two level pooled fluid in every batch | 3 Serum calibrators cups X 4 time replicate and 3 QC cups X 4 time replicate and 3 blank cups X 4 time replicate | y=ax | ||
| Fluid Protein (Biuret) | When Lot changes | Two level pooled fluid in every batch | 3 Serum calibrators cups X 4 time replicate and 3 QC cups X 4 time replicate and 3 blank cups X 4 time replicate | y=ax | ||
| Fluid Protein (Pyrogallol Red) | When Lot changes | Two level pooled fluid in every batch | 10 serum Calibrators cups X 2 time replicate and 5 blank cups X 2 time replicate | y=ax | ||
| Serum Ketone bodies | Qualitative Test | Two level pooled sera( Normal and Acetone spiked) once a day | NA | |||
| Urine Ketone bodies | Qualitative Test | Two level pooled urine ( Normal and Acetone spiked) once a day | NA | |||
| Urine Calcium | When Lot changes | 1 level pooled spiked urine once when test done | 3 Serum calibrators cups X 4 time replicate and 3 QC cups X 4 time replicate and 3 blank cups X 4 time replicate | y=ax | ||
| Urine Creatinine | When Lot changes | 1 level pooled spiked urine once when test done | 3 Serum calibrators cups X 4 time replicate and 3 QC cups X 4 time replicate and 3 blank cups X 4 time replicate | y=ax | ||
| Urine Phosphorous | When Lot changes | 1 level pooled spiked urine once when test done | 3 Serum calibrators cups X 4 time replicate and 3 QC cups X 4 time replicate and 3 blank cups X 4 time replicate | y=ax | ||
| Urine Sodium | When Lot changes | 1 level pooled spiked urine once when test done | 3 Serum calibrators cups X 4 time replicate and 3 QC cups X 4 time replicate and 3 blank cups X 4 time replicate | y=ax | ||
| Urine Protein (Biuret) | When Lot changes | 1 level pooled spiked urine once when test done | 3 Serum calibrators cups X 4 time replicate and 3 QC cups X 4 time replicate and 3 blank cups X 4 time replicate | y=ax | ||
| Urine Protein (Pyrogallol Red) | When Lot changes | 1 level pooled spiked urine once when test done | 10 serum Calibrators cups X 2 time replicate and 5 blank cups X 2 time replicate | y=ax | ||
| Urine Uric Acid (Pyrogallol Red) | When Lot changes | 1 level pooled spiked urine once when test done | 3 Serum calibrators cups X 4 time replicate and 3 QC cups X 4 time replicate and 3 blank cups X 4 time replicate | y=ax | ||
| Protein electrophoresis-Detection of M band | Two level QC run with each batch | NA | ||||
| Erba Chem-5 Plus V2 | |||||
|---|---|---|---|---|---|
| Parameter | Calibration | IQC | |||
| When in use(Erba XL-640 breakdown) | When not in use | When in use(Erba XL-640 breakdown) | When not in use | ||
| GLC | Every batch | Every month before External QC and when lot changes | 2 level QC With Every batch | 2 level QC every Sunday | y=ax |
| BIL | when lot changes | Every month before External QC and when lot changes | 2 level QC 3 times a day | 2 level QC every sunday | y=ax |
| CR | when lot changes | Every month before External QC and when lot changes | 2 level QC 3 times a day | 2 level QC every sunday | y=ax |
| ALT | when lot changes | Every month before External QC and when lot changes | 2 level QC 3 times a day | 2 level QC every sunday | y=ax |
| CKMB | Fixed factor | Fixed factor | When new reagent bottle reconstituted, kit control should be measured | Kit control when patient sample is to be done | |
| CHE | When lot changes | Not applicable | Normal or abnormal Qc with patient sample | Not applicable | |
| ADA | in every batch | Not applicable | Two level pooled sera in every batch | Not applicable |
nchsls/c/biochemistry/document/calibration_and_quality_control_frequency.txt · Last modified: 2022/08/13 07:34 (external edit)
