1.PURPOSE OF EXAMINATION To lay down the procedure for Specimen Fixation in Histo-pathology section at NCHLS Surat

2.PRINCIPLE AND METHOD OF PROCEDURE USED FOR EXAMINATION Formalin preserves the tissue in life like state. Approximately 16-24 hrs requires for adequate fixation. The penetration capacity of formalin is 1 cm so large specimens shall be cut into multiple slices of 1 cm thickness.

3.PERFORMANCE CHARACTERISTICS NA

4.TYPE OF SAMPLE Biopsy/ Excised specimen

5.PATIENT PREPARATION NA

6.TYPE OF CONTAINER AND ADDITIVES Plastic close container

7.REQUIRED EQUIPMENT AND REAGENT: 10% Neutral buffer formalin / Bouin’s fluid.

8.ENVIRONMENTAL AND SAFETY CONTROLS Universal safety precautions

9.CALIBRATION PROCEDURE(METROLOGICAL TRACEBILITY) NA

10.PROCEDURE(STEPS)

• For routine histopathological examination specimen should be sliced 1cm apart , put cotton soaked in 10% formalin inside it submerged in 10% buffered formalin (volume of formalin should be 10 times of the specimen) and for frozen section examination tissue should be received in normal saline.
• If the specimen is large. Cut the specimen in smaller slice with proper orientation with 1 cm thickness.
• If fixative is hemorrhagic, change the formalin.
• Keep the received specimen and respective requisition form at proper suitable place.

11.QUALITY CONTROL PROCEDURE NA

12.INTERFERENCE Formalin pigment

13.PRINCIPLE OF PROCEDURE FOR CALCULATING RESULTS INCLUDING, WHERE RELEVENT, THE MEASUREMENT OF UNCERTAINITY OF MEASURED QUANTITY VALUES NA

14.BIOLOGICAL REFERENCE INTERVALS OR CLINICAL DECISION VALUES NA

15.REPORTABLE INTERVAL OF EXAMINATION RESULTS NA

16.INSTRUCTION FOR DETERMINING QUANTITATIVE RESULTS WHEN RESULTS IS NOT WITHIN THE MEASUREMENT INTERVAL NA

17.ALERT/CRITICAL VALUE, WHERE APPROPRIATE Autolysed specimen

18.LABORATORY CLINICAL INTERPRETATION NA

19. POTENTIAL SOURCE OF VARIATION NA

20.REFERENCE Histopathological laboratory techniques by Bancroft, 6th edition