| Histopathology IQC Plan | ||||
|---|---|---|---|---|
| No. of product groups | No. of tests/ calibration parameters | Does the IQC plan broadly cover all tests/calibrations & details thereof | Frequency of IQC checks | Compliance criteria |
| 1.Biopsy (small, medium & large tissues; Slides and blocks for second opinion): | * Grossing * Decalcification * Processing * Paraffin embedding - Microtomy - H&E staining * * Light Microscopy with interpretation | -Lot validation with every change of reagent Lot - Section cutting and staining of each person Every 15 days - Daily QC for H&E staining - Inter observer quality control once in a month | ||
| 2.Histochemistry: | * PAS( Periodic Acid Schiff) stain * Reticulin (Gordon and Sweet’s) stain * Perl’s Prussian blue stain for Iron * ZN (Ziehl Neelsen) stain for AFB * Masson Trichrome stain * Grocott Methanamine Silver Stain * Alcian Blue stain * Toludine Blue - Giemsa (May Grunwald Giemsa) | - IQC with each special stain -interobserver comparision of all special stains every 6 months -Lot validation with every change of reagent lot | ||
| 3.Tissue received in normal saline: | * Grossing * Processing & section cutting by cryostat H & E staining | Slides of each case for frozen section is sent for review by another authorised signatory posted in other section |