1. PURPOSE OF EXAMINATION: For processing the biopsy tissues in a manner so as to ensure optimal results.

2. PRINCIPLE AND METHOD OF PROCEDURE USED FOR EXAMINATION: As per instructions & operating manual of automated tissue processor manufacture.

3. STAFF RESPONSIBLE: Senior technician

4. PERFORMANCE CHARACTERISTICS: NA

5. TYPE OF SAMPLE: Tissue bits (after grossing).

6. PATIENT PREPARATION: NA

7. TYPE OF CONTAINER AND ADDITIVES: Perforated cassettes.

8. REQUIRED EQUIPMENT AND REAGENT :

Equipment: Automatic tissue processor.

Reagent: Alcohol (ascending grades), Xylene, Paraffin.

9. ENVIRONMENATAL AND SAFETY CONTROLS: Universal safety precautions

10. CALIBRATION PROCEDURE: calibration done once in a year through EQDC.

11. PROCEDURE:

By automated tissue processor:

• First lift up the lead of tissue processor (1st container) & hang the tissue basket in hook present in lead

• Switch on the UPS

• Timer is preset as per follow:

      1.10% formalin -5 to 6 hrs

      2.50% alcohol – 1hr

      3.60% alcohol – 1hr

      4.70% alcohol – 1hr

      5.80% alcohol – 1hr

      6.90% alcohol – 1hr

      7.100% absolute alcohol -1hr

      8. Xylene 1-1 ½ hr

      9. Xylene 2-1 ½ hr

     10. Paraffin wax 1 -1hr

     11. Paraffin wax 2-1hr

• After 16 hrs (next day) switch off the instrument and take the tissue basket from processor.

Manual Tissue Processing:

1.Take 2 glass beaker of 500 ml capacity and add 70% acetone in first one and 80% of acetone in second beaker. put tissue cassette in first beaker for 30 minute

2.After 30 minute remove tissue bucket from first bucket and put in second bucket for 30minute.

3.Replace acetone of first bucket with 90% acetone.

4.After 30 minute remove tissue bucket from second bucket and put in first bucket of 90% acetone for 30minute.

5.Replace acetone of first bucket with 100% acetone.

6.After 30 minute remove tissue bucket from first bucket and put in second bucket of 100% acetone for 30minute.

7.After these steps tissue cassettes are kept in xylene for two changes each for 1 hour.

8.Then cassettes are transferred to wax bath of histokinet.

9.Wax bath is connected with electric power; wait till temperature reach at 65 C. put bucket inside for one hour.

10.After 1 ½ hour remove cassettes from it add new wax in wax bath and again keep cassettes for 1 ½ hour.

11.Tissue is ready for embedding.

12.QUALITY CONTROL PROCEDURE: NA

13.INTERFERENCE: NA

14. PRINCIPLE OF PROCEDURE FOR CALCULATING RESULTS INCLUDING, WHERE RELEVENT, THE MEASUREMENT OF UNCERTAINITY OF MEASURED QUANTITY VALUES: NA

15. BIOLOGICAL REFERENCE INTERVALS OR CLINICAL DECISION VALUES: NA

16. REPORTABLE INTERVAL OF EXAMINATION RESULTS: NA

17. INSTRUCTION FOR DETERMINING QUANTITATIVE RESULTS WHEN RESULTS IS NOT WITHIN THE MEASUREMENT INTERVAL: NA

18. ALERT/ CRITICAL VALUE, WHERE APPROPRIATE: NA

19. LABORATORY CLINICAL INTERPRETATION: NA

20. POTENTIAL SOURCE OF VARIATION: NA

21. REFERENCE: Histopathological laboratory techniques by Bancroft, 6th edition.