1. PURPOSE OF EXAMINATION: To lay down the procedure for preparation of 10% buffered formalin &Bouin’s fluid for tissue fixation in Histo-pathology section at NCHLS Surat.
2. PRINCIPLE AND METHOD OF PROCEDURE USED FOR EXAMINATION: Process by which the constituents of the cells and therefore, of tissues, are fixed in a chemical and partly also in a physical state so that they will withstand subsequent treatment with various reagents with a minimum loss, significant distortion or decomposition and keep the tissue in as life like a manner as possible.
3. STAFF RESPONSIBLE: lab. Technician under supervision of resident doctor/tutor.
4. PERFORMANCE CHARACTERISTICS: NA
5. TYPE OF SAMPLE: NA
6. PATIENT PREPARATION:NA
7. TYPE OF CONTAINER AND ADDITIVES: Clean, labeled plastic close container
8. REQUIRED EQUIPMENTAND REAGENTS:
9. ENVIRONMENTAL AND SAFETY CONTROLS: Universal safety precautions
Buffered Formalin Preparation: (20 liter buffered formalin)
Bouin’s Fluid Preparation: 100 ml
10. QUALITY CONTROL PROCEDURE:NA
11. INTERFERENCE: Inadequate quality/improper dilution
12. PRINCIPLE OF PROCEDURE FOR CALCULATING RESULTS INCLUDING, WHERE RELEVENT, THE MEASUREMENT OF UNCERTAINITY OF MEASURED QUANTITY VALUES: NA
13. BIOLOGICAL REFERENCE INTERVALS OR CLINICAL DECISION VALUES OR CLINICAL DECISION VALUES:NA
14. REPORTABLE INTERVAL OF EXAMINATION RESULTS: NA
15. INSTRUCTION FOR DETERMINING QUANTITATIVE RESULTS WHEN RESULTS IS NOT WITHIN THE MEASUREMENT INTERVAL:NA
16. ALERT /CRITICAL VALUES WHERE APPROPRIATE: NA
17. LABORATORY CLINICAL INTERPRETATION:NA
18. POTENTIAL SOURCE OF VARIATION: NA
19. REFERENCE: Histopathological laboratory techniques by Bancroft, 6th edition