Staining procedure:

Take test slide on which staining has to be performed along with positive control.

Deparaffinize section by keeping in oven for 5min and clear it with 2 changes of xylene each for 15 minute.

Bring section to water.

Stain in freshly prepared 1% toludine blue in 50% isopropyle alcohol for 30 minute at 370C.

Blot with tissue paper and place in a isopropyle alcohol for 1 minute.

Dehydrate in alcohol, clear in xylene, mount with DPX