Staining procedure Dewax section by keeping in oven for 5 minute, clear with 2 changes of xylene each for 15 minute,

dehydrate with graded alcohol 2 changes each for 2 minute

Bring section to water.

Oxidize with 1% acidified potassium permanganate solution for 5 minutes.

Wash in tap water and bleach with 1% oxalic acid solution for 1min.

Wash in running tap water then Rinse in distilled water.

Sensitize in 2.5% iron alum solution for 15minutes.

Wash well in several changes of distilled water.

Impregnate with ammoniacal silver solution for 2 minute in coplin jars

Wash well in several changes of distilled water

Reduce in 10% formalin for 2 minute.

Rinse in tap water.

Treat with 5% sodium thiosulphate solution for 3 minutes.

Rinse in tap water.

Dehydrate ,clear and mount by DPX.