Cryostat is switched on from the previous night (5-6 hrs before) of planned OT and temperature is monitored.

Received specimen is given a frozen no, which is also tagged to the patient requisition form.

If more than one specimen of the sane patient is sent for frozen section, they are labeled in alphabetical order( eg. FS 1A, FS 1B, FS 1c..)

Place the representative tissue on a metal cryostat chunck after applying 1-2drops of tissue adherent(gum) on the chunck.

Pour 1-2 drops of normal saline on the tissue.

Put the chunck with the tissue on a metal tray of the cryostat for freezing the tissue.

When the temperature of the cryostat attains -250C to -300C, trim the tissue to get full exposure.

Cut the section at 5 micron thickness and pick up from the knife to put it on previously labelled glass slides coated with egg albumin.

Remaining tissue bit and uncut thawed frozen bits are placed in a container with 10% buffered formalin. A HP no. is then given to this specimen.

After completion of procedure, defrost the cryostat and clean it with Hypochlorite solution, trimmings and blade are removed.

Oiling of machine is done , then switch off the instrument.