Take test slide on which staining has to be performed along with positive control.

Deparaffinize section by keeping in oven for 5 minute and clear it with 2 changes xylene each for 15 minute.

Dehydration with graded alcohol 2 change for 2 min.

Bring section to water.

Put filter paper on the slide section and pour the CF solution over the section.

Heat section with spirit lamp till steam arises. Allow it to heat for 5 minute.

Wash in tap water to remove excess stain.

Differentiate with 20% sulfuric acid till section decolorize section till tissue is very pale pink Wash in water.

Counter stain in 1%methylene blue 1 dip.

Wash in water.

clear in xylene, mount with DPX