STOCK SOLUTION
Buffer pH 6.8 –

              KH2PO4 (9.1 G) + D.W. (1016 ml)
		+
	Na2HPO4.2H2O (11.5 g) + D.W.( 984 ml)

Buffer pH 7.0 -

              KH2PO4 (3.5 G) + D.W. (389 ml)
		+
	NaH2PO4.2H2O (7.1 g) + D.W.( 611 ml)

Buffer pH 7.2 -

              KH2PO4 (2.5 G) + D.W. (280 ml)
		+
	NaH2PO4.2H2O (8.4 g) + D.W.( 720 ml)

WORKING SOLLUTION:
For Working solution of buffer ( 25ml of stock solution + 475 ml D.W.)
Buffer pH 6.8 – For Bone marrow staining
Buffer pH 7.0 – For P.S. staining
Buffer pH 7.2 – For Malarial parasite staining

Dilution:
For B.M. Staining - 1:6.5 ( 10 ml Geimsa Stain + 55 ml Buffer water)
For P.S. Staining – 1:5 ( 10 ml Geimsa Stain + 40 ml Buffer water)

PREPARATION OF STAIN:
• Take 5 gm of gimsa powder and crush it properly, then mix it in 500 ml of glycerol and keep it for 48 hours.
• Add & well mix 500 ml of absolute methanol in the above mentioned solution and keep at room temperature for one week.
• After filtering it is stored in amber colored bottle at room temperature

STAINING PROCEDURE:
• Add buffer( 6.8- 7.2 pH) in the filtered stain in 1:4 dilution
• Arrange thick/ thin smears on double rod stand.
• Pour the stain-buffer mixture on the arranged slides. Leave it for 10-15 minutes
• Stain over the slides should be mixed by blowing with pipette.
• After 10-15 minutes, wash the slides in slowly running tap water.
• Dry the washed slides in air at room temperature.