START UP
-Switch on main power supply
-Switch on UPS
-Start CPU and Monitor
-Open CDM 5.1 program
-Switch on VCS(VARIANT II CHROMATOGRAPHIC SYSTEM)
-Switch on VSS(VARIANT II SAMPLING STATION)
-Click on variant II β thal Inactive mode
-Click on variant II β thal Inactive mode,it prompt return to active,then click yes
-So,the variant II β thal comes on ready mode,ok it by cliking on it
-Click Run
-In Run click worklist
-Wait upto green light
-When shows green light,click on start option
-Then machine is ready to run
-Load sample into the system racks

-place the following in the sample rack:

-Once the run is finished ,note retention time of A2 and temperature.
-After making the temperature adjustment,wait 10-15 min for temperature to equilibrate and start sample run.

SHUT DOWN:
-Once the run is finished,as indicated by 'STOP'barcoded tube in rack
-Close CDM 5.1 program
-Switch turn off on both monitor and CPU
-Switch turn off on VCS and VSS
-Switch turn off on UPS
-Switch turn off on main power supply.

MAINTENANCE
*DAILY LOG CHECKLIST

Prerun check:
-Buffer and wash bottle levels
-Pressure check pump A
-Pressure check pump B
-Piston seal wash:inspect tubing for liquid(add DI water if needed)
-Paper supply
-Leaks
-Cartridge injection count
-Waste container level

Postrun check:
-Remove samples
-Wipe spills

*MONTHLY MAINTAINANCE CHECKLIST

1)Clean /Decontaminate sampling fluid path:
-Disconnect cartridge to detector tubing from the cartridge holder
-Remove the analytic cartridge
-Clean the cartridge holder with a cotton swab and DI water
-Install the plastic PEEK dummy cartridge into holder
-Install decon tubing to the outlet of the cartridge holder
-Place outside of decon tubing into a breaker to collect wastw from decontamination procedure.
-Select the V2-DECON test from the SETUP/Test screen
-Click ok on the test dialog box
-Place five sample microvials of 5%sodium hypochlorite solution into the adapters in the first five position of a sample rack
-In the last five position of the rack,place five sample microvail with DI water
-Put the stop tube with an empty sample microvail into the second sample rack and place both sample racks on the sampling station
-Start worklist
-When the status returns to READY,remove the plastic PPEK dummy cartridge,clean the cartridge holder again with a cotton swab and DI water,then reinstall original analytic cartridge
-Remove decon tubing from the cartridge holder.Discard breaker waste.
-Reconnect cartridge to detector tubing,securing both tubing end connection
-Remove the racks.select the desired test from the SETUP/Test screen.

2)Clean the Flow cell
-The flow cell cleaning kit contains a syringe,flow cell cleaning liquid and tubing assemblies:
-Make sure the variant II is in the READY mode.open the front panel of the VCS and disconnect the fingertight fitting connected to the outlet of the column thermomodule
-Remove the syringe,adapter ang union fitting from the kit.connect the adapter to the union fitting.connect a syringe filled with cleanng solution to the adapter.
-Using the syringe ,push about 20ml of solution through the flow cell.let stand for atleast 20 min.repeat once.
-Fill the syringe with DI water.using the syringe ,push about 20 ml of DI water through flow cell to rinse out the cleaning solution.Repeat once.
-Remove the union fitting,adapter,syringe used for cleaning and reconnect the tubing for the thermomodule outlet.

3)Flush piston/Seal valve

4)Clean Exterior/Interior surface/Belts
Exterior surface cleaning:Use a cloth or sponge dampened with water to wipe the surface.
Interior surface cleaning:Wipe up any fluid,using a soft disposable towel or tissue.clean the interior base plate of the system by wiping it with a soft cloth or sponge dampened with water.
Belts can be actuated using the cleaning selection in the MAINTAIN/Instrument screen.

5)Clean Barcode Reader
-Remove racks from the VSS.
-Using a soft cotton cloth,gently wipe the barcode reader face.

6)Clean Dilution chamber
-Power off the VSS.
-Remove the needle cover door
-Push the needle gantry back,cleaning the needle from being over the dilution chamber
-clean the dilution chamber using cotton swabs and DI water
-Replace needle cover door
-Power on VSS,waiting for sampler to complete intialization and flush cycle
-Click ok to CDM communication error box,sampler resetting box and rack resetting box,returning the system back to ready state.

7)Clean and Inspect Sample Racks with a cotton swab

8)Clean Sample Needle
-From CDM,go to the MAINTAIN/Instrument screen.
-Under sampler ,choose the replace needle button
-After the needle is repositioned ,open the automatic sampler cover of the sampling station
-clean the sample needle with a cloth,removing debris.
-Close the cover to the sample needle.Power should be restored to the unit.Choose the move needle home button.The needle will return to its home position and a message will appear asking if you wish to flush the sampler.It is recommended that you perform this flush.