START:- 1. Switch on main power supply
2. Switch on UPS
3. Switchthe instrument on by pressing the button at the rear of the instrument.
4. Wait during initialization.
5. Switch on the printer.
6. After that login to application , to login into application select username and enter password .
7. Press OK
8. After that click on start up icon for start up pass .
9. Wait until the cycle is over. cycle takes approx 1 minute
10. . Blank cycles (cycles without any blood specimen) are performed during the startup cycle.
11. After start up pass, instrument is “ready” for sample and control run.

SAMPLE PROCESSING:-
If start up is passed we can run the sample.
To Run the Specimen in STAT Mode
1. Press STAT Mode to open the tube holder door.
2. Enter information about the sample.
3. Press Validate in the contextual toolbar.
4. Press Icon (Next analysis) on function toolbar for sampling needle to come out.
5. Gently and thoroughly mix the sample.
6. open the tube and place it below the sampling needle, lift it so that the needle can sample its content.
7. press the manual sampling bar, or press “validate”
8. Remove the tube and put the cap back on once needle has moved up.

SHUTDOWN:-
A shutdown cycle has to be from every 24 hrs.
It is highly recommended to perform a shutdown cycle before switching off the instrument.
1. Press shutdown icon to perform a Shutdown cycle manually.
2. Wait while the instrument shuts down. Shut down takes about 10 min.
3. Press Software exit Icon on contextual toolbar
4. Switch off the instrument
5. Switch off the printer

A. Concentration cleaning- to clean the counting chambers and hydraulic parts.
1. Press Concentrated Cleaning.
2. A window informing you to connect Minoclair and press validate
3. Disconnect the “ABX cleaner luer connected'
4. Connect ABX minoclair luer connector instead
5. Press validate in the contextual toolbar
6. Wait until the cycle is over
A concentration cleaning cycle takes approx. 10 min.
7. When a window informing you to reconnect a reagent and press “validate”, disconnect the ABX minoclair luer connecter and connect ABY Cleaner luerconnector instead
8. Press validate in the contextual toolbar.
9. Startup cycle and an nalysis on a control bood sample

B. Auto clean Cycle
• Give the counting chambers a quick cleaning.
1. Press Autoclean.
2. Wait until the cycle is over
An Autoclean cycle takes about 3 MINUTES

C. Backflush
Clean the counting chamber apertures in case of blockage.
1. Press “ BackFlush RBC/PLT” to backflush the RBC/PLT aperture
2. Wait for the instrument to complete the backflush cycle which takes about 35 sec.
3. Press “ backflush LMNEB” to backflush the flowcell
4. Wait for the instrument to complete the backflush cyde, which takes about 1 min.

D. To prime a reagent
1. Select the reagent you want to prime in Priming/Unpriming area
2. Press prime
3. Wait Until the cycle is over.

E. To Unprime a reagent
1. Disconnect and remove the bottle of reagents You want to unprime.
2. Select the reagents you want to unprime in the Priming /unpriming area
3. Press Unprime
4. Wait until the cycle is over

F. Rinse the system
LMNE flow cells can sometimes be defective due to airbubbles and/or blockage, this allows to fix both these issues.
1. Press the rinse in the cleaning area
2. Wait for the instrument to complete the rinsing cycle, which takes approx. 40 sec.