FORWARD PIPETTING:

 Press the operating button to the first stop.

 Dip the tip attached to the pipette in to the solution to a depth of about 1 cm and slowly release the operating button. Wait for a while, and then withdraw it from the liquid touching it against the edge of the reservoir to remove excess liquid adhering to the outer surface of the tip.

 Dispense the liquid in to the receiving vessel by gently pressing the operating button to the first stop. After a second, press the operating button to the second stop. This will empty the tip completely. Remove the pipette from the vessel, sliding it up the wall of vessel.

 Release the operating button to the ready position.

REVERSE PIPETTING:

 Press the operating button to the second stop.

 Dip the tip attached to the pipette in to the solution to a depth of about 1 cm and slowly release the operating button. This action will fill the tip with a volume that is larger then the set volume. Wait 1-2 seconds and withdraw the tip from the liquid, touching it against the edge of the reservoir to remove excess liquid

 Dispense the liquid in to the receiving vessel by gently pressing the operating button to the first stop. This volume is equal to the set volume. Hold the button in this position. Some liquid will remain in the tip, which should not be dispensed.

 The liquid remaining in the tip can be dispensed back in to the original solution by pressing the button to the second stop or disposed together with the tip.

 Release the operating button to the ready position.

PIPETTING RECOMMENDATIONS:

 Aspirate liquid into the pipette only when a tip is attached to its tip cone.

 Allow liquids, tips & pipettes to equilibrate to the ambient temperature.

 Pre-rinsing of tip 5 times with the liquid to be dispensed is recommended. This is important especially when dispensing liquids which have viscosity & density different from water.

 Pre-rinse the tip several times before use when pipetting liquids at temperature different from ambient.

 While pipetting, the pipettor should be vertically straight & tip should be dipped only a few millimeters into the liquid.

 Always control the push button movements with the thumb for consistency.

 Wipe the tip only if there is liquid on the outside of the tip, being careful to avoid touching the tip’s orifice.

 Do not keep pipette in your hand while not working, to avoid transferring of body heat.

 Using excessive force to turn the push button outside the range specified for it may jam the mechanism and damage the pipettor.

CLEANING YOUR PIPETTOR:

 To clean your pipettor, use ethanol and a soft cloth or lint free tissue. It is recommended to clean the tip cone regularly.

IN HOUSE MAINTENANCE:

 Hold down the ejector

 Press the tooth of the opening tool between the tip ejector and the tip ejector collar.

 Place the wrench end of the opening tool over the tip cone and turn it anticlockwise.

 After removing the tip cone, wipe the piston, the O-ring and the tip cone with ethanol and a soft cloth.

 Before replacing the tip cone, it is recommended to grease the piston slightly using the silicon grease provided

 After reassembling, use the pipettor (without liquid) several times to make sure that the grease is spread evenly.