USG GUIDED FINE NEEDLE ASPIRATION CYTOLOGY
1.PURPOSE: To prepare the Smears as per standard text literature & manufacture’s guidelines.
2. STAFF RESPONSIBLE: resident doctor.
3. PRINCIPLE: NA
4. PERFORMANCE SPECIFICATION: NA
5. SAMPLE: NA
6. TYPE OF CONTAINER:
7. EQUIPMENT AND REAGENT USED:
EQUIPMENTS:
Standard 18/22 Gauze, 90 mm disposable lumbar puncture needle with trochar.
10 cc Disposable Syringes.
Disposable slides.
0.2mm cover slip.
Diamond pencil for labeling the slides.
REAGENTS:
Fixative-Acetone free methanol – Extra pure
Stains-H & E. Stain, Giemsa Stain, PAP Stain
9. CALIBRATION PROCEDURE: When new residents come, they observe the procedure of FNAC for 3 days and then they perform FNAC under the observation of a senior resident for 4 days. After 1 wk of training, they are allowed to perform FNAC independently.
10. PROCEDURE (STEPS):
PATIENT PREPARATION:
A Clear explanation of procedure to patient to get his/her written consent and cooperation.
Procedure is usually carried out as per position given by radiologist to the patient.
Disinfect the skin using the spirit swab.
Pre aspiration sedation is sometime necessary and the only in deep aspiration in anxious or agitated patients.
PROCEDURE:
Experienced radiologist find out lesion where FNAC has to be done.
With the help of USG probe, operator position the needle within the target tissue.
Pull the trochar to apply the negative pressure.
Move the needle back and forth inside the target, so good part of aspirate is often drawn into the needle.
Release the negative pressure while needle remains in target tissue and withdraw the needle.
Needle is detached and air is drawn into syringe.
Blow sample on microscopy slide and with the help of other slides, spread the material.
11. TAT FOR TEST: ½ hour
12. LABORATORY INTERPRETATION: NA
13. SAFETY PRECAUTIONS: Universal safety precaution (Biosafety manual).
14. VALIDATION OF RESULTS TO BE DONE BY: By authorized signatories & Assi. Technical manager.
15. REVIEW, RECORDS & RECOMMENDATION: As per ISO 15189 Guidelines Clause 4.13 & NABL 112
16. REFERENCE: Fine Needle Aspiration Cytology, 4th edition, Svante R. Orell