Take heat fixed test slide on which staining has to be performed along with positive control.

Put filter paper on the slide smear and pour the CF solution over the section.

Heat section with spirit lamp till steam arises. Allow it to heat for 5 minute.

Wash in tap water to remove excess stain.

Differentiate with 20% sulfuric acid till smear decolorizes and is pale pink.

Wash in water.

Counter stain in 1%methylene blue 1 dip.

Wash in water.

clear in xylene, mount with DPX